Basal and metal‐induced expression of metallothionein isoform 1 and 2 genes in the RWPE‐1 human prostate epithelial cell line

The human prostate gland has low basal expression of the metallothionein‐1 and ‐2 proteins. In prostate cancer, MT‐1/2 protein expression is variable and correlates directly with the increasing Gleason score of the tumor. The goal of the present study was to determine if the RWPE‐1 cell line is a good model to elucidate the mechanisms underlying the alterations in MT‐1/2 expression that occur during the development of prostate cancer. It was shown that the RWPE‐1 cell line and in situ prostate tissue have identical expression profiles of MT‐1 and MT‐2 isoform‐specific mRNAs (MT‐1E, MT‐1X and MT‐2A) and similar levels of MT‐1/2 protein. It was also shown that the RWPE‐1 cells respond to Zn+2 and Cd+2 exposure by induction of the basally expressed MT mRNAs and the accumulation of high levels MT‐1/2 protein (in excess of 10% of total protein). It was also shown that additional MT‐1 mRNAs were expressed when the cells were exposed to either metal; MT‐1A, MT‐1F, MT‐G and MT‐1H for Cd+2‐exposed cells; and, MT‐1F, MT‐G and MT‐1H for Zn+2‐exposed cells. The results suggest that RWPE‐1 cells may be a valuable system to define the interplay between Zn+2 concentration, Cd+2 exposure and MT in normal and malignant prostate epithelial cells. Copyright © 2007 John Wiley & Sons, Ltd.

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