Estrogen receptor cytochemistry by fluorescent estrogen.

A recently synthesized fluorescein-labeled estrogen (17FE, 1-(N)-fluoresceinyl-estrone-thiosemicarbazone) interacts with estrogen-target cells like the native hormone and visualizes the uptake, transport, and distribution of estrogen in intact target cells. Moreover, estrogen binding sites are traced by 17FE in cryostat sections of estrogen target tissues as well. Cell and tissue 17FE binding sites fulfill the accepted criteria for specific estrogen receptors (finite binding capacity, high affinity, steroid and tissue specificity). This fluorescent probe allows estrogen receptors to be studied in a wide variety of cell and tissue preparations under varying conditions of physiologic and pathophysiologic interest.

[1]  I. Nenci,et al.  Intracellular flow of particulate steroidreceptor complexes in steroid target cells , 1980, Virchows Archiv. B, Cell pathology including molecular pathology.

[2]  B. Rao,et al.  Steroid-albumin conjugate interaction with steroid-binding proteins. , 1980, Endocrinology.

[3]  A. Howell,et al.  Histological detection of oestrogen receptor in human breast carcinomas. , 1980, Lancet.

[4]  H. Wotiz,et al.  Kinetics of estradiol entry into uterine cells. , 1979, Endocrinology.

[5]  P. Rolland,et al.  A preliminary report on the use of immunoperoxidases to study binding of estrogens in rat uteri. , 1979, Journal of steroid biochemistry.

[6]  A. Traish,et al.  Comparison of formation, activation, and nuclear translocation of receptor . estradiol (R . E2) complex at 0 degrees C and 37 degrees C in intact uterine cells. , 1979, The Journal of biological chemistry.

[7]  S. H. Lee,et al.  Cancer cell estrogen receptor of human mammary carcinoma , 1979, Cancer.

[8]  I. Nenci.  Estrogen receptor immunocytochemistry. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[9]  E. Baulieu.  Cell membrane, a target for steroid hormones , 1978, Molecular and Cellular Endocrinology.

[10]  D. Brigati,et al.  Detection of androgen and estrogen receptors in human prostatic carcinoma and hyperplasia by fluorescence microscopy. , 1978, Research communications in chemical pathology and pharmacology.

[11]  I. Nenci.  Receptor and centriole pathways of steroid action in normal and neoplastic cells. , 1978, Cancer Research.

[12]  L. Sternberger,et al.  Estrogen receptor immunocytochemistry. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  A. C. Carter,et al.  Immunofluorescent detection of estrogen receptors in breast cancer. Comparison with dextran‐coated charcoal and sucrose gradient assays , 1978, Cancer.

[14]  Strobl Js,et al.  Studies of steroid hormone effects on human breast cancer cells in long-term tissue culture. , 1978 .

[15]  S. J. Higgins,et al.  Molecular mechanisms of steroid hormone action. , 1978, Advances in cancer research.

[16]  G. Greene,et al.  Antibodies to estrogen receptor: immunochemical similarity of estrophilin from various mammalian species. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[17]  E. Jensen,et al.  The diagnostic implications of steroid binding in malignant tissues. , 1977, Advances in clinical chemistry.

[18]  I. Nenci,et al.  In vivo and in vitro immunofluorescent approach to the physiopathology of estradiol kinetics in target cells. , 1976, Journal of steroid biochemistry.

[19]  W. Dandliker,et al.  Hormone binding by cells and cell fragments as visualized by fluorescence microscopy. , 1976, Research communications in chemical pathology and pharmacology.

[20]  I. Nenci,et al.  Detection and dynamic localisation of estradiol-receptor complexes in intact target cells by immunofluorescence technique. , 1976, Journal of steroid biochemistry.

[21]  W. Stumpf.  Nuclear concentration of 3H-estradiol in target tissues. Dry-mount autoradiography of vagina, oviduct, ovary, testis, mammary tumor, liver and adrenal. , 1969, Endocrinology.

[22]  E. Jensen,et al.  A two-step mechanism for the interaction of estradiol with rat uterus. , 1968, Proceedings of the National Academy of Sciences of the United States of America.