Intestinal infection due to enteroaggregative Escherichia coli among human immunodeficiency virus-infected persons.

To investigate the pathogenic role of enteroaggregative Escherichia coli (EAggEC) among human immunodeficiency virus-infected persons, 111 outpatients with and 68 without diarrhea were evaluated. Examination of stool samples included the HeLa cell adherence assay and an EAggEC polymerase chain reaction (PCR) assay using primers complementary for the plasmid locus CVD432. The pCVD432 genotype, adherence phenotype, and patient characteristics were correlated with occurrence of diarrhea by multivariate analyses. EAggEC PCR and adherence assays were positive in 7 (6%) and 24 (22%) patients with diarrhea and in 1 (1%) and 21 (31%) asymptomatic control patients, respectively. Clinical manifestations associated with EAggEC PCR-positive isolates were nonspecific; EAggEC infections were independent of CD4 lymphocyte counts. Of the pCVD432 genotype, 5 (71%) of 7 were resistant to cotrimoxazole and ampicillin, and 1 strain was resistant to ciprofloxacin. Overall, pCVD432 PCR-positive E. coli was the most prevalent intestinal organism associated with diarrhea. The adherence assay results did not correlate with diarrhea.

[1]  I. Okeke,et al.  Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in Southwest Nigeria. , 2000, The Journal of infectious diseases.

[2]  R. Weber,et al.  Enteric infections and diarrhea in human immunodeficiency virus-infected persons: prospective community-based cohort study. Swiss HIV Cohort Study. , 1999, Archives of internal medicine.

[3]  A. Telenti,et al.  Clinical progression and virological failure on highly active antiretroviral therapy in HIV-1 patients: a prospective cohort study , 1999, The Lancet.

[4]  E. Presterl,et al.  Enteroaggregative and Enterotoxigenic Escherichia coli among Isolates from Patients with Diarrhea in Austria , 1999, European Journal of Clinical Microbiology and Infectious Diseases.

[5]  R. Guerrant,et al.  Enteroaggregative Escherichia coli. , 2001, Emerging infectious diseases.

[6]  D. Acheson,et al.  Successful treatment of diarrheal disease associated with enteroaggregative Escherichia coli in adults infected with human immunodeficiency virus. , 1998, The Journal of infectious diseases.

[7]  R. Zbinden,et al.  Enteroaggregative Escherichia coli as a potential cause of diarrheal disease in adults infected with human immunodeficiency virus. , 1998, The Journal of infectious diseases.

[8]  H. Karch,et al.  Acute and chronic diarrhoea and abdominal colic associated with enteroaggregative Escherichia coli in young children living in western Europe , 1997, The Lancet.

[9]  M. Levine,et al.  Enteroaggregative Escherichia coli heat-stable enterotoxin is not restricted to enteroaggregative E. coli. , 1996, The Journal of infectious diseases.

[10]  Alimuddin Zumla,et al.  HEp-2 cell-adherent Escherichia coli in patients with human immunodeficiency virus-associated diarrhea. , 1995, The Journal of infectious diseases.

[11]  J. Heesemann,et al.  Development of PCR for screening of enteroaggregative Escherichia coli , 1995, Journal of clinical microbiology.

[12]  M. Levine,et al.  Heterogeneity of enteroaggregative Escherichia coli virulence demonstrated in volunteers. , 1995, The Journal of infectious diseases.

[13]  D. Lüscher,et al.  Detection of shigellae, enteroinvasive and enterotoxigenic Escherichia coli using the polymerase chain reaction (PCR) in patients returning from tropical countries. , 1994, Molecular and cellular probes.

[14]  T. Yamamoto,et al.  Drug resistance and adherence to human intestines of enteroaggregative Escherichia coli. , 1992, The Journal of infectious diseases.

[15]  M. Levine,et al.  A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. , 1990, The Journal of infectious diseases.