In vivo oxygen sensing using lifetime based photoacoustic measurements

Hypoxia is a condition where a region of tissue has less than adequate oxygen. It is of particular importance in tumor biology, as the hypoxic core of tumors has been shown to impede the effectiveness of many therapies. We demonstrate a novel method for oxygen sensing in vivo, based on the photoacoustic lifetime measurement of an oxygen sensitive probe. The experimental results derived from the main artery in the rat tail indicated that the lifetime of the probe, quantified by the photoacoustic measurement, shows a good linear relationship with the blood oxygenation level in the targeted artery.

[1]  R. Mason,et al.  Proton imaging of siloxanes to map tissue oxygenation levels (PISTOL): a tool for quantitative tissue oximetry , 2008, NMR in biomedicine.

[2]  Xu Xiao.  Photoacoustic imaging in biomedicine , 2008 .

[3]  D. Boas,et al.  Dendritic phosphorescent probes for oxygen imaging in biological systems. , 2009, ACS Applied Materials and Interfaces.

[4]  Kenneth A Krohn,et al.  Molecular Imaging of Hypoxia , 2008, Journal of Nuclear Medicine.

[5]  Shai Ashkenazi,et al.  Photoacoustic probing of fluorophore excited state lifetime with application to oxygen sensing. , 2008, Journal of biomedical optics.

[6]  John Humm,et al.  Iodine-124-labeled iodo-azomycin-galactoside imaging of tumor hypoxia in mice with serial microPET scanning , 2003, European Journal of Nuclear Medicine and Molecular Imaging.

[7]  David A Boas,et al.  Optical monitoring of oxygen tension in cortical microvessels with confocal microscopy. , 2009, Optics express.

[8]  R. Durand.  Keynote address: the influence of microenvironmental factors on the activity of radiation and drugs. , 1991, International journal of radiation oncology, biology, physics.

[9]  G. Semenza,et al.  Regulation of hypoxia-induced angiogenesis: a chaperone escorts VEGF to the dance. , 2001, The Journal of clinical investigation.

[10]  Oren Sagher,et al.  Targeted blue nanoparticles as photoacoustic contrast agent for brain tumor delineation , 2011, Nano research.

[11]  D. Brizel,et al.  Prognostic value of tumor oxygenation in 397 head and neck tumors after primary radiation therapy. An international multi-center study. , 2005, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[12]  R. Hill,et al.  Hypoxia induces DNA overreplication and enhances metastatic potential of murine tumor cells. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Sergei A Vinogradov,et al.  Oxygen distribution in murine tumors: characterization using oxygen-dependent quenching of phosphorescence. , 2005, Journal of applied physiology.

[14]  Richard M. Schwartzstein,et al.  Respiratory Physiology: A Clinical Approach , 2005 .

[15]  Sergei A Vinogradov,et al.  Oxygen pressures in the interstitial space and their relationship to those in the blood plasma in resting skeletal muscle. , 2006, Journal of applied physiology.

[16]  Aniruddha Ray,et al.  Lifetime-based photoacoustic oxygen sensing in vivo. , 2012, Journal of biomedical optics.

[17]  Shai Ashkenazi,et al.  Photoacoustic lifetime imaging of dissolved oxygen using methylene blue. , 2010, Journal of biomedical optics.

[18]  James L Tatum,et al.  Hypoxia: Importance in tumor biology, noninvasive measurement by imaging, and value of its measurement in the management of cancer therapy , 2006, International journal of radiation biology.

[19]  Sergei A Vinogradov,et al.  Oxyphor R2 and G2: phosphors for measuring oxygen by oxygen-dependent quenching of phosphorescence. , 2002, Analytical biochemistry.