Phase separation in frozen erythrocyte membrane preparations

The reversible formation of a lipid‐like phase in frozen preparations of erythrocyte membranes has been studied by X‐ray diffraction and by electron microscopy of freeze‐fracture replicas. The observations provide strong evidence for lateral migration or displacement at specific temperatures of intra‐membrane particles. This creates large areas of particle‐free membranes which fracture preferentially so as to dominate the freeze‐fracture image.

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