论文信息 - Myocardial apoptotic index based on in situ DNA nick end-labeling of endomyocardial biopsies does not predict prognosis of dilated cardiomyopathy.

Myocardial apoptotic index based on in situ DNA nick end-labeling of endomyocardial biopsies does not predict prognosis of dilated cardiomyopathy.

BACKGROUND DNA breaks detected largely by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) are observed in the hearts of patients with diseases such as dilated cardiomyopathy (DCM). STUDY OBJECTIVES To determine the prognostic value of TUNEL assays in cases of DCM. DESIGN, SETTING, AND PARTICIPANTS DCM patients were selected from among patients who had undergone left ventricular (LV) biopsy during the period from 1994 to 2001 in our hospital. Of those, 46 (35 men and 11 women; mean [+/- SD] age, 58 +/- 11 years) who were followed up for > 3 years after the undergoing the biopsy (mean follow-up period, 4.9 +/- 2.0 years) or died during the follow-up period were entered into the present study. The myocardial apoptotic index was assessed in deparaffinized biopsy specimens that were stained using a conventional TUNEL assay. In addition, all surviving patients received a follow-up echocardiographic examination. RESULTS Ten of the 46 biopsy specimens (22%) contained TUNEL-positive myocytes; their mean apoptotic index was 0.44 +/- 1.05%. The apoptotic index showed no relation to cardiac functional parameters determined at the time of biopsy, however. Seven patients died during the follow-up period, and 19 of the surviving patients were readmitted to the hospital because of a worsening of their heart failure. There was no significant difference in the apoptotic indexes of biopsy specimens from the dead and surviving patients, or between the surviving patients who were readmitted to the hospital and those who were not. There was also no significant correlation between the apoptotic index and changes in the LV ejection fraction, LV end-diastolic diameter, or LV posterior wall thickness during follow-up. CONCLUSION The apoptotic index derived from TUNEL assays is not predictive of the prognosis of patients with DCM-induced heart failure.

[1] R. Virmani,et al. Apoptosis in myocytes in end-stage heart failure. , 1996, The New England journal of medicine.

[2] J. Schaper,et al. Unresolved issues regarding the role of apoptosis in the pathogenesis of ischemic injury and heart failure. , 2000, Journal of molecular and cellular cardiology.

[3] P. Kang,et al. Apoptosis and heart failure: A critical review of the literature. , 2000, Circulation research.

[4] C A Beltrami,et al. Apoptosis in the failing human heart. , 1997, The New England journal of medicine.

[5] S. Ben‐Sasson,et al. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation , 1992, The Journal of cell biology.

[6] P. Hornsby,et al. Presence of double-strand breaks with single-base 3' overhangs in cells undergoing apoptosis but not necrosis , 1996, The Journal of cell biology.