Surface enhanced fluorescence effect improves the in vivo detection of amyloid aggregates.

The β-amyloid (Aβ) peptide is one of the key etiological agents in Alzheimer's disease (AD). The in vivo detection of Aβ species is challenging in all stages of the illness. Currently, the development of fluorescent probes allows the detection of Aβ in animal models in the near-infrared region (NIR). However, considering future applications in biomedicine, it is relevant to develop strategies to improve detection of amyloid aggregates using NIR probes. An innovative approach to increase the fluorescence signal of these fluorophores is the use of plasmonic gold nanoparticles (surface-enhanced fluorescence effect). In this work, we improved the detection of Aβ aggregates in C. elegans and mouse models of AD by co-administering functionalized gold nanorods (GNRs-PEG-D1) with the fluorescent probes CRANAD-2 or CRANAD-58, which bind selectively to different amyloid species (soluble and insoluble). This work shows that GNRs improve the detection of Aβ using NIR probes in vivo.

[1]  Tianshu Wu,et al.  Research Advances on the Adverse Effects of Nanomaterials in a Model Organism, Caenorhabditis elegans , 2021, Environmental toxicology and chemistry.

[2]  E. Giralt,et al.  In vivo micro computed tomography detection and decrease in amyloid load by using multifunctionalized gold nanorods: a neurotheranostic platform for Alzheimer's disease. , 2021, Biomaterials science.

[3]  M. Kogan,et al.  Plasmonic Nanoparticles as Optical Sensing Probes for the Detection of Alzheimer’s Disease , 2021, Sensors.

[4]  A. deMello,et al.  In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice , 2020, bioRxiv.

[5]  Xiaoyan Dong,et al.  Design of Multifunctional Agent Based on Basified Serum Albumin for Efficient In Vivo β-Amyloid Inhibition and Imaging. , 2020, ACS applied bio materials.

[6]  F. Albericio,et al.  Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence , 2020, Nanomaterials.

[7]  Y. Pang,et al.  Biological applications of near infrared fluorescence dye probes in monitoring Alzheimer's disease. , 2019, European journal of medicinal chemistry.

[8]  D. Mcclements,et al.  Nanoemulsion-based delivery systems for testing nutraceutical efficacy using Caenorhabditis elegans: Demonstration of curcumin bioaccumulation and body-fat reduction. , 2019, Food research international.

[9]  R. Gao,et al.  Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer's disease , 2019, Theranostics.

[10]  Yinhui Li,et al.  Versatile fluorescent probes for near-infrared imaging of amyloid-β species in Alzheimer's disease mouse model. , 2019, Journal of materials chemistry. B.

[11]  R. Castellani,et al.  The amyloid cascade and Alzheimer’s disease therapeutics: theory versus observation , 2019, Laboratory Investigation.

[12]  Kamila Czarnecka,et al.  New Perspectives of Alzheimer Disease Diagnosis - the Most Popular and Future Methods. , 2018, Medicinal chemistry (Shariqah (United Arab Emirates)).

[13]  E. Giralt,et al.  Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. , 2017, Nanomedicine.

[14]  E. Giralt,et al.  Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease. , 2017, Nanomedicine : nanotechnology, biology, and medicine.

[15]  Jian-feng Li,et al.  Plasmon-enhanced fluorescence spectroscopy. , 2017, Chemical Society reviews.

[16]  E. Mandelkow,et al.  Tau in physiology and pathology , 2015, Nature Reviews Neuroscience.

[17]  M. Chalfie,et al.  A Transparent Window into Biology: A Primer on Caenorhabditis elegans , 2015, Genetics.

[18]  Wei Wang,et al.  Near-infrared fluorescent probes for imaging of amyloid plaques in Alzheimer׳s disease , 2015, Acta pharmaceutica Sinica. B.

[19]  Zeng Li,et al.  Design and synthesis of curcumin analogues for in vivo fluorescence imaging and inhibiting copper-induced cross-linking of amyloid beta species in Alzheimer's disease. , 2013, Journal of the American Chemical Society.

[20]  E. Giralt,et al.  Stable conjugates of peptides with gold nanorods for biomedical applications with reduced effects on cell viability. , 2013, ACS applied materials & interfaces.

[21]  R. Aroca,et al.  Experimental confirmation of local field enhancement determining far-field measurements with shell-isolated silver nanoparticles. , 2012, Small.

[22]  B. Gulyás,et al.  Development of a Small D-Enantiomeric Alzheimer’s Amyloid-β Binding Peptide Ligand for Future In Vivo Imaging Applications , 2012, PloS one.

[23]  Yu-jiong Wang,et al.  A multifunctional peptide rescues memory deficits in Alzheimer's disease transgenic mice by inhibiting Aβ42-induced cytotoxicity and increasing microglial phagocytosis , 2012, Neurobiology of Disease.

[24]  Yoshio Kobayashi,et al.  Rapid one-step synthesis, characterization and functionalization of silica coated gold nanoparticles , 2011 .

[25]  N. Inestrosa,et al.  Copper Reduces Aβ Oligomeric Species and Ameliorates Neuromuscular Synaptic Defects in a C. elegans Model of Inclusion Body Myositis , 2011, The Journal of Neuroscience.

[26]  T. Zako,et al.  Amyloid oligomers: formation and toxicity of Aβ oligomers , 2010, The FEBS journal.

[27]  B. Bacskai,et al.  Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. , 2009, Journal of the American Chemical Society.

[28]  C. Masters,et al.  Intracellular amyloid formation in muscle cells of Aβ-transgenic Caenorhabditis elegans: determinants and physiological role in copper detoxification , 2009, Molecular Neurodegeneration.

[29]  T. van Groen,et al.  Reduction of Alzheimer’s Disease Amyloid Plaque Load in Transgenic Mice by D3, a D‐Enantiomeric Peptide Identified by Mirror Image Phage Display , 2008, ChemMedChem.

[30]  R. V. Van Duyne,et al.  Localized surface plasmon resonance spectroscopy and sensing. , 2007, Annual review of physical chemistry.

[31]  Theresa Stiernagle.  Maintenance of C. elegans. , 2006, WormBook : the online review of C. elegans biology.

[32]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[33]  Yoo-Hun Suh,et al.  Amyloid Precursor Protein, Presenilins, and α-Synuclein: Molecular Pathogenesis and Pharmacological Applications in Alzheimer's Disease , 2002, Pharmacological Reviews.

[34]  Claudio Soto,et al.  Reduction of amyloid load and cerebral damage in transgenic mouse model of Alzheimer's disease by treatment with a β‐sheet breaker peptide , 2002, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[35]  R. Weissleder.  A clearer vision for in vivo imaging , 2001, Nature Biotechnology.

[36]  C. Link,et al.  In Vivo Aggregation of β‐Amyloid Peptide Variants , 1998 .

[37]  D R Wekstein,et al.  Linguistic ability in early life and cognitive function and Alzheimer's disease in late life. Findings from the Nun Study. , 1996, JAMA.

[38]  C. Link,et al.  Expression of human beta-amyloid peptide in transgenic Caenorhabditis elegans. , 1995, Proceedings of the National Academy of Sciences of the United States of America.