Detection of malignant hyperthermia susceptibility using a spin label technique on red blood cells.

Using spin labelled red blood cells and electron paramagnetic resonance spectroscopy (EPR), we observed that halothane 3 mmol litre-1 produced a much greater decrease in the rotational correlation time of red blood cells from individuals who were at risk for malignant hyperthermia (MH), compared with normals. Subsequently we performed blind tests on 14 individuals whose MH susceptibility status had been determined on the basis of in vitro contracture studies. When compared with the results of the in vitro contracture studies, the EPR studies predicted correctly the patients' status in 13 of the 14 individuals.

[1]  G. Pezeshkpour,et al.  Calcium uptake in frozen muscle biopsy sections compared with other predictors of malignant hyperthermia susceptibility. , 1987, Anesthesiology.

[2]  S. Ohnishi Effects of Alcohol and Halothane on the Structure and Function of Sarcoplasmic Reticulum a , 1987 .

[3]  B. Britt,et al.  ANAESTHETIC-INDUCED INCREASE IN IONISED CALCIUM IN BLOOD MONONUCLEAR CELLS FROM MALIGNANT HYPERTHERMIA PATIENTS , 1987, The Lancet.

[4]  S. Ohnishi Effects of halothane, caffeine, dantrolene and tetracaine on the calcium permeability of skeletal sarcoplasmic reticulum of malignant hyperthermic pigs. , 1987, Biochimica et biophysica acta.

[5]  S. Ohnishi MEMBRANES OF SARCOPLASMIC RETICULUM AND RED BLOOD CELLS MAY HAVE A COMMON GENE: A NEW POSSIBILITY OF USING BLOOD FOR NON–INVASIVE SCREENING FOR MALIGNANT HYPERTHERMIA , 1987 .

[6]  S. Ohnishi,et al.  Abnormal membrane properties of the sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia: modes of action of halothane, caffeine, dantrolene, and two other drugs. , 1986, Archives of biochemistry and biophysics.

[7]  M. Walsh,et al.  Electrophoresis of Muscle Proteins Is Not a Method for Diagnosis of Malignant Hyperthermia Susceptibility , 1986, Anesthesiology.

[8]  Scott Ea,et al.  Failure of the platelet-halothane nucleotide depletion test as a diagnostic or screening test for malignant hyperthermia. , 1986 .

[9]  F. Ellis European Malignant Hyperpyrexia Group. , 1984, British journal of anaesthesia.

[10]  N. Masson,et al.  Platelet Model for Halothane‐Induced Effects on Nucleotide Metabolism Applied to Malignant Hyperthermia , 1984, Acta anaesthesiologica Scandinavica.

[11]  G. Gronert,et al.  Calcium‐induced Ca2+ release from sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia , 1983, FEBS letters.

[12]  S. Ohnishi,et al.  Measurement of red cell sickling: a method for studying the efficacy of antisickling drugs under physiological conditions. , 1983, Canadian journal of physiology and pharmacology.

[13]  L. Boxer,et al.  Fluidity properties and liquid composition of erythrocyte membranes in Chediak-Higashi syndrome , 1981, The Journal of cell biology.

[14]  G. A. Gronert Malignant hyperthermia , 1989, Anesthesiology.

[15]  J. Edmonson,et al.  Screening for malignant hyperthermia with a platelet bioassay. , 1980 .

[16]  D. Simpson,et al.  Internal microviscosity of red blood cells and hemoglobin-free resealed ghosts: a spin-label study. , 1979, Biochemistry.

[17]  K. Mabuchi,et al.  Use of cryostat sections for measurement of Ca2+ uptake by sarcoplasmic reticulum. , 1978, Analytical biochemistry.

[18]  L. Dalton,et al.  Erythrocyte membrane abnormalities in Duchenne muscular dystrophy monitored by saturation transfer electron paramagnetic resonance spectroscopy. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[19]  E. Beutler,et al.  The removal of leukocytes and platelets from whole blood. , 1976, The Journal of laboratory and clinical medicine.