Isolation and mass spectroscopic characterization of phytochemicals from the bark of Acacia leucophloea (Roxb.) Willd.

ABSTRACT Acacia leucophloea is a medicinal plant used traditionally for treating many diseases. In the present study, phytochemicals were isolated from the bark of A. leucophloea using multiple chromatographic techniques. The separation and isolation were done using column chromatography. The molecular masses of isolated compounds were assigned by electro spray ionization mass spectrometry. The subsequent structure characterization was carried out by a tandem mass spectrometric method. Fragmentation behavior of compounds was investigated using hexapole collision cell mass spectrometry in negative mode. Six phenolic compounds such as catechin, leuco-fisetinidin, gallic acid, ferulic acid, syringic acid, and O-methyl epicatechin were identified. Most of the compounds identified are first report from this plant.