Heparin facilitates the extraction of tissue fibronectin.

Extraction of fibronectin from two human tissues, lung parenchyma and placental villi, was facilitated by the incorporation of heparin into extraction media. The effect of heparin was additive to the effect of urea which is known to extract fibronectin. These experiments provide further evidence that fibronectin and glycosaminoglycans are associated in connective tissues and the use of heparin forms the basis for a simple method for extraction and quantitation of tissue fibronectin.

[1]  L. Lorand,et al.  Fibronectin-mediated uptake of gelatin-coated latex particles by peritoneal macrophages , 1980, The Journal of cell biology.

[2]  E. Engvall,et al.  Complexing of fibronectin glycosaminoglycans and collagen. , 1980, Biochimica et biophysica acta.

[3]  K. Yamada,et al.  Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. , 1980, The Journal of biological chemistry.

[4]  M. Höök,et al.  Heparin enhances the rate of binding of fibronectin to collagen. , 1980, The Biochemical journal.

[5]  T. Ji,et al.  Cross-linking of fibronectin to sulfated proteoglycans at the cell surface , 1979, Cell.

[6]  K. Alitalo,et al.  Isolation of the pericellular matrix of human fibroblast cultures , 1979, The Journal of cell biology.

[7]  Kenneth M. Yamada,et al.  Fibronectins—adhesive glycoproteins of cell surface and blood , 1978, Nature.

[8]  D. Mosher,et al.  High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in maglinant transformation , 1978 .

[9]  D. J. Rose,et al.  Crossed immunoelectrophoresis from sodium dodecyl sulfate-polyacrylamide gels into antibody-containing agarose: an improved method for evaluation of the number of immunochemical determinants in polypeptides, with reference to spectrin. , 1978, Analytical biochemistry.

[10]  I. Pastan,et al.  Characterization of a major fibroblast cell surface glycoprotein. , 1977, Biochemistry.

[11]  R. Hynes,et al.  Extensive disulfide bonding at the mammalian cell surface. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[12]  A. Vaheri,et al.  Dimeric character of fibronectin, a major cell surface-associated glycoprotein. , 1977, Biochemical and biophysical research communications.

[13]  Carolyn A. Converse,et al.  Membrane protein analysis by two-dimensional immunoelectrophoresis. , 1975, Science.

[14]  C. Laurell,et al.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. , 1966, Analytical biochemistry.