Hot dogs: Thermography in the assessment of stress in dogs (Canis familiaris)—A pilot study

Infrared thermography (IRT) represents a non-invasive method to investigate stress responses in animals. Despite the large existing literature about stress responses in dogs, the potential use of IRT in assessing dogs' stress reactions has not been investigated so far. This study evaluates the usefulness of IRT to assess dogs' emotional responses to an unpleasant and stressful event. After a preliminary test, aimed to evaluate the correlation between eye temperature and rectal temperature in dogs in a stressful situation, a sample of 14 adult healthy dogs was observed during a standardized veterinary examination, carried out by an unfamiliar veterinarian in the presence of their owners. Dogs' behaviors and eye temperatures were recorded before the start of the veterinary visit, during, and after the clinical examination. Dogs' levels of activity and stress-related behaviors varied across the different phases of the visit. Interestingly, the dogs showed an increase in eye temperature during the examination phase compared with both pre-examination and post-examination phases, despite a concomitant significant decrease in their level of activity. However, it also emerged that the thermographic camera, although remote and non-invasive, was disturbing for the dogs, to some extent, as they showed avoidance behaviors, including averting their gaze and/or turning their head, exclusively when the thermographic camera was oriented to them. Overall results suggest that IRT may represent a useful tool to investigate emotional psychogenic stress in dogs. Nevertheless, further research is needed to establish the specificity and sensitivity of IRT in this context and to assess how different dogs' characteristics, breed, previous experience, and the nature and severity of the stressor could influence the magnitude and type of the stress response.

[1]  Ádám Miklósi,et al.  The behavior of the domestic dog (Canis familiaris) during separation from and reunion with the owner: A questionnaire and an experimental study , 2011 .

[2]  Bonne Beerda,et al.  Manifestations of chronic and acute stress in dogs , 1997 .

[3]  Á. Miklósi,et al.  Consistency of dogs’ reactions to threatening cues of an unfamiliar person , 2008 .

[4]  A. Schaefer,et al.  Using eye temperature and heart rate for stress assessment in young horses competing in jumping competitions and its possible influence on sport performance. , 2013, Animal : an international journal of animal bioscience.

[5]  Gustav Fischer Verlag,et al.  INFRARED THERMOGRAPHY : PRINCIPLES AND APPLICATIONS , 1998 .

[6]  Á. Miklósi,et al.  Wolves do not join the dance: Sophisticated aggression control by adjusting to human social signals in dogs , 2013 .

[7]  Bonne Beerda,et al.  Behavioural and Hormonal Indicators of Enduring Environmental Stress in Dogs , 2000, Animal Welfare.

[8]  T. Kikusui,et al.  Objective measurement of fear-associated learning in dogs , 2006 .

[9]  H. Küchenhoff,et al.  Fear-related behaviour of dogs in veterinary practice. , 2009, Veterinary journal.

[10]  G. Chrousos,et al.  The hypothalamic-pituitary-adrenal (HPA) axis: A major mediator of the adaptive responses to stress , 2003 .

[11]  Pascal Carrive,et al.  Changes in cutaneous and body temperature during and after conditioned fear to context in the rat , 2005, The European journal of neuroscience.

[12]  Paola Valsecchi,et al.  Behavioral and physiological responses of guide dogs to a situation of emotional distress , 2007, Physiology & Behavior.

[13]  R. Paylor,et al.  The stress-induced hyperthermia paradigm as a physiological animal model for anxiety: A review of pharmacological and genetic studies in the mouse , 2007, Neuroscience & Biobehavioral Reviews.

[14]  N. Waran,et al.  Efficacy of dog appeasing pheromone in reducing stress and fear related behaviour in shelter dogs , 2005 .

[15]  P. Alm.  Stuttering, emotions, and heart rate during anticipatory anxiety: a critical review. , 2004, Journal of fluency disorders.

[16]  Clara Palestrini,et al.  Heart rate and behavioural responses of dogs in the Ainsworth's Strange Situation: A pilot study , 2005 .

[17]  R. Schenkel,et al.  Submission: Its Features and Function in the Wolf and Dog , 1967 .

[18]  M. Verga,et al.  Technical note: Applicability of infrared thermography as a non invasive measurements of stress in rabbit , 2010 .

[19]  John A. Basarab,et al.  The use of infrared thermography as an early indicator of bovine respiratory disease complex in calves , 2007, Research in Veterinary Science.

[20]  K. Schwartzkopf-Genswein,et al.  The use of infrared thermography to assess inflammation associated with hot-iron and freeze branding in cattle , 1997 .

[21]  M. Hennessy.  Using hypothalamic–pituitary–adrenal measures for assessing and reducing the stress of dogs in shelters: A review , 2013 .

[22]  C. Kalkman,et al.  Stress-induced hyperthermia and infection-induced fever: Two of a kind? , 2009, Physiology & Behavior.

[23]  F. Toates.  Biological Psychology: An Integrative Approach , 2001 .

[24]  Chérie E. Part,et al.  Physiological, physical and behavioural changes in dogs (Canis familiaris) when kennelled: Testing the validity of stress parameters , 2014, Physiology & Behavior.

[25]  S. Siegel,et al.  Nonparametric Statistics for the Behavioral Sciences , 2022, The SAGE Encyclopedia of Research Design.

[26]  C. A. Hussni,et al.  Thermography and ultrasonography in back pain diagnosis of equine athletes , 2006 .

[27]  L. Romero.  Fight or Flight Responses , 2010 .

[28]  M. Washburn,et al.  Bodily Changes in Pain, Hunger, Fear, and Rage. , 1917 .

[29]  V. Baron,et al.  Adrenocortical and metabolic responses to ACTH injection in horses An assessment by salivary cortisol and infrared thermography of the eye , 2001 .

[30]  K. Stafford,et al.  Non-invasive measurement of stress in dairy cows using infrared thermography , 2007, Physiology & Behavior.

[31]  Marc Bekoff,et al.  Encyclopedia of Animal Behavior , 2004 .

[32]  Márta Gácsi,et al.  A friend or an enemy? Dogs' reaction to an unfamiliar person showing behavioural cues of threat and friendliness at different times , 2005 .

[33]  C. Hall,et al.  An assessment of the aversive nature of an animal management procedure (clipping) using behavioral and physiological measures , 2013, Physiology & Behavior.

[34]  C. M. Coppens,et al.  Neuroendocrinology of coping styles: Towards understanding the biology of individual variation , 2010, Frontiers in Neuroendocrinology.

[35]  Cook Nj,et al.  Nutritional therapy modulates stress responses of elk (Cervus elaphus canadensis) to removal of velvet antler , 2006 .

[36]  Paul S. Morley,et al.  Thermographic Eye Temperature as an Index to Body Temperature in Ponies , 2011 .

[37]  N. Rooney,et al.  Repetitive behaviour in kennelled domestic dog: Stereotypical or not? , 2014, Physiology & Behavior.

[38]  J. Serpell.  The domestic dog : its evolution, behaviour, and interactions with people , 2017 .

[39]  K. Stafford,et al.  Eye temperature and heart rate variability of calves disbudded with or without local anaesthetic , 2008, Physiology & Behavior.

[40]  T. Hori,et al.  Mechanisms and Mediators of Psychological Stress-Induced Rise in Core Temperature , 2001, Psychosomatic medicine.

[41]  James R. Webster,et al.  Infrared thermography as a non-invasive tool to study animal welfare , 2005, Animal Welfare.

[42]  Bonne Beerda,et al.  Behavioural, saliva cortisol and heart rate responses to different types of stimuli in dogs , 1998 .

[43]  N. Emery,et al.  The eyes have it: the neuroethology, function and evolution of social gaze , 2000, Neuroscience & Biobehavioral Reviews.

[44]  A. Schaefer,et al.  Changes in Eye Temperature and Stress Assessment in Horses During Show Jumping Competitions , 2012 .

[45]  A. D. Kennedy,et al.  Daily variation in the udder surface temperature of dairy cows measured by infrared thermography: Potential for mastitis detection , 2003 .

[46]  F. Pirrone,et al.  Evaluation of physiological and behavioral stress-dependent parameters in agility dogs , 2011 .

[47]  Terry L. Stanford.  Behavior of dogs entering a veterinary clinic , 1981 .

[48]  Takeshi Kaneko,et al.  Psychological stress activates a dorsomedial hypothalamus-medullary raphe circuit driving brown adipose tissue thermogenesis and hyperthermia. , 2014, Cell metabolism.

[49]  F. Biondi,et al.  Infrared ocular thermography in dogs with and without keratoconjunctivitis sicca. , 2015, Veterinary ophthalmology.

[50]  Anna Kis,et al.  Man’s Underground Best Friend: Domestic Ferrets, Unlike the Wild Forms, Show Evidence of Dog-Like Social-Cognitive Skills , 2012, PloS one.

[51]  Paul H. Hemsworth,et al.  Fear of novel and startling stimuli in domestic dogs , 2003 .

[52]  Ioannis Pavlidis,et al.  Seeing through the face of deception , 2002, Nature.

[53]  M. Sleeman,et al.  Starving for ghrelin. , 2014, Cell metabolism.

[54]  C. Hall,et al.  Assessing ridden horse behavior: Professional judgment and physiological measures , 2014 .

[55]  G. Chrousos,et al.  The biobehavioral consequences of psychogenic stress in a small, social primate (Callithrix jacchus jacchus) , 1996, Biological Psychiatry.