Identification and characterisation of lactic acid bacteria isolated from traditional Urfa cheese

In this study, characterisation of dominant strains of lactic flora in traditional Urfa cheese made from sheep's milk was performed using biochemical, phenotypic and genotypic methods. According to the results obtained, the percentage distributions of the lactic acid bacteria isolated were as follows: 48.95% Enterococcus spp., 40.55% Lactococcus spp., 9.10% Lactobacillus spp., 0.69% Streptococcus spp. and 0.69% Leuconostoc spp. The majority of lactococcal isolates showed an atypical phenotype of growing in the presence of 6.5% NaCl. Acidification and bacteriocin production were also determined for each isolate. Four strains showed bacteriocin activity.

[1]  P. Fox,et al.  Genotypic characterization of lactic acid bacteria isolated from traditional Pecorino Siciliano cheese , 2008 .

[2]  B. Ozer,et al.  Microflora and Pathogen Bacteria (Salmonella, Klebsiella, Yersinia, Pseudomonas, Aeromonas, Escherichia coli, Staphylococcus aureus) In Urfa Cheese (A Traditional White-Brined Turkish Cheese) , 2008 .

[3]  L. Cocolin,et al.  Microbiological characterization of artisanal Raschera PDO cheese: analysis of its indigenous lactic acid bacteria. , 2008, Food microbiology.

[4]  L. Aquilanti,et al.  Phenotypic, genotypic and technological characterization of predominant lactic acid bacteria in Pecorino cheese from central Italy , 2007, Journal of applied microbiology.

[5]  P. L. Manachini,et al.  Phenotypic typing, technological properties and safety aspects of Lactococcus garvieae strains from dairy environments , 2007, Journal of applied microbiology.

[6]  T. Aymerich,et al.  Tracing Salmonella in Alheira processing plants , 2007, Journal of applied microbiology.

[7]  J. Ogier,et al.  Biodiversity of Bacterial Ecosystems in Traditional Egyptian Domiati Cheese , 2006, Applied and Environmental Microbiology.

[8]  A. González-Córdova,et al.  Characterization of the natural microflora of artisanal Mexican Fresco cheese , 2006 .

[9]  L. Aquilanti,et al.  Resident lactic acid bacteria in raw milk Canestrato Pugliese cheese , 2006, Letters in applied microbiology.

[10]  E. Vaughan,et al.  Artisanal and experimental Pecorino Siciliano cheese: microbial dynamics during manufacture assessed by culturing and PCR-DGGE analyses. , 2006, International journal of food microbiology.

[11]  T. Zotta,et al.  Discrimination of commercial Caciocavallo cheeses on the basis of the diversity of lactic microflora and primary proteolysis , 2005 .

[12]  J. M. Poveda,et al.  Phenotypic and genotypic characterization of lactobacilli isolated from Spanish goat cheeses. , 2005, International journal of food microbiology.

[13]  P. Saris,et al.  Nisin-Producing Lactococcus lactis Strains Isolated from Human Milk , 2004, Applied and Environmental Microbiology.

[14]  G. Giraffa Functionality of enterococci in dairy products. , 2003, International journal of food microbiology.

[15]  P. L. Manachini,et al.  Genetic characterization of some lactic acid bacteria occurring in an artisanal protected denomination origin (PDO) Italian cheese, the Toma piemontese , 2003 .

[16]  A. Grandison,et al.  Textural and microstructural properties of urfa cheese (a white-brined Turkish cheese) , 2003 .

[17]  A. Caridi Identification and first characterization of lactic acid bacteria isolated from the artisanal ovine cheese Pecorino del Poro , 2003 .

[18]  R. Coppola,et al.  Survey of lactic acid bacteria during the ripening of Caciocavallo cheese produced in Molise , 2003 .

[19]  E. Tsakalidou,et al.  Citrate Metabolism by Enterococcus faecalis FAIR-E 229 , 2001, Applied and Environmental Microbiology.

[20]  T. Beresford,et al.  Recent advances in cheese microbiology , 2001 .

[21]  J. Swings,et al.  Phenotypic and genetic diversity of enterococci isolated from Italian cheeses , 2001, Journal of Dairy Research.

[22]  F. Villani,et al.  Detection and characterization of a bacteriocin, garviecin L1‐5, produced by Lactococcus garvieae isolated from raw cow’s milk , 2001, Journal of applied microbiology.

[23]  R. Çibik,et al.  Molecular diversity of leuconostoc mesenteroides and leuconostoc citreum isolated from traditional french cheeses as revealed by RAPD fingerprinting, 16S rDNA sequencing and 16S rDNA fragment amplification. , 2000, Systematic and applied microbiology.

[24]  B. Moreno,et al.  Lactic acid bacteria isolated from a hand-made blue cheese , 2000 .

[25]  G. Suzzi,et al.  Phenotypic and genotypic diversity of yeasts isolated from water‐buffalo Mozzarella cheese , 2000, Journal of applied microbiology.

[26]  B. Mayo,et al.  Biochemical and microbiological characterization of artisanal `Peñamellera' cheese: analysis of its indigenous lactic acid bacteria , 1999 .

[27]  P. Cocconcelli,et al.  Molecular and physiological characterization of dominant bacterial populations in traditional Mozzarella cheese processing , 1999, Journal of applied microbiology.

[28]  A. Eldar,et al.  Biodiversity of Lactococcus garvieaeStrains Isolated from Fish in Europe, Asia, and Australia , 1999, Applied and Environmental Microbiology.

[29]  S. Pochet,et al.  Influence of Pasteurization and Fat Composition of Milk on the Volatile Compounds and Flavor Characteristics of a Semi-hard Cheese , 1998 .

[30]  Guyot,et al.  Preliminary characterization of microflora of Comté cheese , 1998, Journal of applied microbiology.

[31]  P. Cocconcelli,et al.  Characterization of the lactic acid bacteria in artisanal dairy products , 1997, Journal of Dairy Research.

[32]  M. Gasson,et al.  Isolation of nisin-producing Lactococcus lactis strains from dry fermented sausages. , 1995, The Journal of applied bacteriology.

[33]  Vos,et al.  Detection and characterization of lactose-utilizing Lactococcus spp. in natural ecosystems , 1995, Applied and environmental microbiology.

[34]  G. Venema,et al.  Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid , 1989, Applied and environmental microbiology.

[35]  E. Franciosi,et al.  Biodiversity and technological potential of wild lactic acid bacteria from raw cows' milk , 2009 .

[36]  M. A. Herreros,et al.  Technological characterization of lactic acid bacteria isolated from Armada cheese (a Spanish goats' milk cheese) , 2003 .

[37]  T. Müller Comparison of methods for differentiation between homofermentative and heterofermentative lactic acid bacteria , 1990 .