Activation and inhibition of the transduction process in silkmoth olfactory receptor neurons.

Electrophysiological responses of olfactory receptor neurons in both male and female silkmoths (Bombyx mori) were investigated. In both sexes, the G-protein activator sodium fluoride and 1,2-dioctanoyl-sn-glycerol, a membrane-permeable analog of the protein kinase C activator diacylglycerol, elicited nerve impulse responses similar to those elicited by weak continuous stimulation with odorants. Therefore, G(q)-proteins and diacylglycerol-activated ion channels seem to be involved in the transduction process in both pheromone-sensitive neurons in males and general odorant-sensitive neurons in females. Decyl-thio-trifluoro-propanone is known to inhibit electrophysiological responses of male moths to pheromones, but has no effect in females. Application of this inhibitor reduced the frequency, but not the amplitude of elementary receptor potentials. It had no inhibitory effect on nerve impulse responses elicited by sodium fluoride or 1,2-dioctanoyl-sn-glycerol. This supports the idea that decyl-thio-trifluoro-propanone acts on a prior step of the transduction cascade, e.g. on the pheromone receptor molecules. General odorants, such as (+/-)-linalool and 1-heptanol, excite olfactory receptor neurons in females, but inhibit the pheromone-sensitive neurons in males. Both (+/-)-linalool and 1-heptanol inhibited the responses of male neurons elicited by sodium fluoride or 1,2-dioctanoyl-sn-glycerol. (+/-)-Linalool reduced the amplitude of elementary receptor potentials. In contrast to decyl-thio-trifluoro-propanone, (+/-)-linalool and 1-heptanol seem to interfere with later processes of the transduction cascade, possibly the opening of ion channels.

[1]  R. Steinbrecht,et al.  Topochemistry of moth olfactory sensilla , 1997 .

[2]  G. Ziegelberger,et al.  Decyl-thio-trifluoropropanone, a competitive inhibitor of moth pheromone receptors , 2000, Journal of Comparative Physiology A.

[3]  F. Zufall,et al.  From odor and pheromone transduction to the organization of the sense of smell , 2001, Trends in Neurosciences.

[4]  Andrey Rzhetsky,et al.  A Spatial Map of Olfactory Receptor Expression in the Drosophila Antenna , 1999, Cell.

[5]  E. Miyachi,et al.  Direct suppression by odorants of cyclic nucleotide-gated currents in the newt photoreceptors , 2000, Brain Research.

[6]  K. Kaissling,et al.  Die Reaktionsweise und das Reaktionsspektrum von Riechzellen bei Antheraea pernyi (Lepidoptera, Saturniidae) , 1964, Zeitschrift für vergleichende Physiologie.

[7]  K. Kaissling,et al.  Olfactory perireceptor and receptor events in moths: a kinetic model. , 2001, Chemical senses.

[8]  K. Kaissling,et al.  Effects of temperature on silkmoth olfactory responses to pheromone can be simulated by modulation of resting cell membrane resistances , 1996, Journal of Comparative Physiology A.

[9]  G. Ziegelberger,et al.  On the function of the pheromone binding protein in the olfactory hairs of Antheraea polyphemus , 1991 .

[10]  T. Heinbockel,et al.  VARIABILITY OF OLFACTORY RECEPTOR NEURON RESPONSES OF FEMALE SILKMOTHS (BOMBYX MORI L.) TO BENZOIC ACID AND ()-LINALOOL , 1996 .

[11]  F. Zufall,et al.  Dual activation of a sex pheromone-dependent ion channel from insect olfactory dendrites by protein kinase C activators and cyclic GMP. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[12]  H. Breer,et al.  Rapid kinetics of second messenger formation in olfactory transduction , 1990, Nature.

[13]  M. Stengl,et al.  Inositol-trisphosphate-dependent calcium currents precede cation currents in insect olfactory receptor neurons in vitro , 1994, Journal of Comparative Physiology A.

[14]  R Maida,et al.  Identification of PLCβ and PKC in pheromone receptor neurons of Antheraea polyphemus , 2000, Neuroreport.

[15]  K. Kaissling,et al.  Responses of bombykol receptor cells to (Z,E)-4,6-hexadecadiene and linalool , 1989, Journal of Comparative Physiology A.

[16]  K. Kaissling,et al.  Interactions of pheromone with moth antennae: Adsorption, desorption and transport , 1985 .

[17]  K. Kaissling,et al.  Elementary Receptor Potentials of Insect Olfactory Cells , 1994 .

[18]  K. Kaissling,et al.  Die Riechschwelle des Seidenspinners , 2004, Naturwissenschaften.

[19]  K. Kaissling.  Peripheral mechanisms of pheromone reception in moths. , 1996, Chemical senses.

[20]  G. Stange,et al.  The site of action of general anaesthetics in insect olfactory receptor neurons. , 1995, Chemical senses.

[21]  K. Kaissling,et al.  Single unit and electroantennogram recordings in insect olfactory organs , 1995 .

[22]  John R. Carlson,et al.  A Novel Family of Divergent Seven-Transmembrane Proteins Candidate Odorant Receptors in Drosophila , 1999, Neuron.

[23]  B. Antonny,et al.  The mechanism of aluminum-independent G-protein activation by fluoride and magnesium. 31P NMR spectroscopy and fluorescence kinetic studies. , 1993, The Journal of biological chemistry.

[24]  E. Eguchi,et al.  Atlas of arthropod sensory receptors : dynamic morphology in relation to function , 1999 .

[25]  M. Stengl.  Intracellular-messenger-mediated cation channels in cultured olfactory receptor neurons. , 1993, The Journal of experimental biology.

[26]  A. Redkozubov.  Elementary receptor currents elicited by a single pheromone molecule exhibit quantal composition , 2000, Pflügers Archiv.

[27]  M. Laue,et al.  G-protein activation, identification and immunolocalization in pheromone-sensitive sensilla trichodea of moths , 1997, Cell and Tissue Research.

[28]  Monika Stengl,et al.  Perireceptor Events and Transduction Mechanisms in Insect Olfaction , 1999 .

[29]  O. Vostrowsky,et al.  A new pheromone of the silkworm moth Bombyx mori , 1978, The Science of Nature.