In vitro and in vivo two-photon luminescence imaging of single gold nanorods.

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

[1]  Hiromi Okamoto,et al.  Plasmon mode imaging of single gold nanorods. , 2004, Journal of the American Chemical Society.

[2]  W. Webb,et al.  Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo , 2003, Science.

[3]  J. Zyss,et al.  Local second-harmonic generation enhancement on gold nanostructures probed by two-photon microscopy. , 2003, Optics letters.

[4]  Yaron Silberberg,et al.  Multiphoton plasmon-resonance microscopy. , 2003, Optics express.

[5]  A. Mooradian,et al.  Photoluminescence of Metals , 1969 .

[6]  Daniel A. Zweifel,et al.  Sulfide-Arrested Growth of Gold Nanorods. , 2005, Chemistry of materials : a publication of the American Chemical Society.

[7]  Shen,et al.  Photoinduced luminescence from the noble metals and its enhancement on roughened surfaces. , 1986, Physical review. B, Condensed matter.

[8]  A Paul Alivisatos,et al.  Gold nanorods as novel nonbleaching plasmon-based orientation sensors for polarized single-particle microscopy. , 2005, Nano letters.

[9]  J. M. Worlock,et al.  Surface picosecond raman gain spectroscopy of a cyanide monolayer on silver , 1979 .

[10]  Lukas Novotny,et al.  Theory of Nanometric Optical Tweezers , 1997 .

[11]  M. Moskovits Surface-enhanced spectroscopy , 1985 .

[12]  Lukas Novotny,et al.  Continuum generation from single gold nanostructures through near-field mediated intraband transitions , 2003 .

[13]  Paul Mulvaney,et al.  Drastic reduction of plasmon damping in gold nanorods. , 2002 .

[14]  V. P. Safonov,et al.  Quantum size effect in two-photon excited luminescence from silver nanoparticles , 2004 .

[15]  E Gratton,et al.  Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. , 1995, Biophysical journal.

[16]  M. El-Sayed,et al.  Some interesting properties of metals confined in time and nanometer space of different shapes. , 2001, Accounts of chemical research.

[17]  Michael Vollmer,et al.  Optical properties of metal clusters , 1995 .

[18]  W. Webb,et al.  Two-Photon Fluorescence Excitation Cross Sections of Biomolecular Probes from 690 to 960 nm. , 1998, Applied optics.

[19]  Thomas A. Klar,et al.  Plasmon emission in photoexcited gold nanoparticles , 2004 .

[20]  V. Rotello,et al.  Nanoparticles: scaffolds and building blocks. , 2003, Accounts of chemical research.

[21]  X. Xie,et al.  Near-field fluorescence microscopy based on two-photon excitation with metal tips , 1999 .

[22]  Catherine J. Murphy,et al.  Seed‐Mediated Growth Approach for Shape‐Controlled Synthesis of Spheroidal and Rod‐like Gold Nanoparticles Using a Surfactant Template , 2001 .

[23]  M. El-Sayed,et al.  The `lightning' gold nanorods: fluorescence enhancement of over a million compared to the gold metal , 2000 .

[24]  Yuen-Ron Shen,et al.  Surface-enhanced Second-harmonic Generation , 1981 .

[25]  W. Webb,et al.  Fluorescence correlation spectroscopy. II. An experimental realization , 1974, Biopolymers.

[26]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[27]  Theo Rasing,et al.  Local-field enhancement on rough surfaces of metals, semimetals, and semiconductors with the use of optical second-harmonic generation , 1984 .

[28]  Jess P. Wilcoxon,et al.  Photoluminescence from nanosize gold clusters , 1998 .