The relationship of modulation of major histocompatibility complex class I antigens to retrovirus transformation in rat cell lines.

The expression of major histocompatibility complex (MHC) Class I antigens has been studied, by means of monoclonal antibodies directed against nonpolymorphic determinants of MHC Class I molecules, in two epithelial differentiated cell lines (FRTL-5 clone 2 and PC clone 3) and in one fibroblast cell line (FRT Fibro) of Fischer rat thyroid origin, before and after infection with various acute retroviruses carrying the v-ras-Ha, v-mos, v-src, polyoma middle T, and c-myc oncogenes. The results obtained indicate that a single virus does not produce identical changes in MHC Class I molecule expression in all tested lines, but a general increase occurs in lines derived from FRTL-5 clone 2 and a decrease occurs in lines derived from PC clone 3 and from FRT Fibro. Thus the modulation of expression seems to proceed always in the same direction in each cell line regardless of the infecting retrovirus and appears to involve posttranscriptional mechanisms, since no modification of expression of mRNA levels has been observed between normal and transformed cells. Only one line of PC clone 3 origin, transformed by the cooperation of two oncogenes (human c-myc and middle T), almost completely lost MHC Class I antigens on the cell surface and presented a significantly reduced synthesis of Class I mRNA.

[1]  A. Fusco,et al.  One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes , 1987, Molecular and cellular biology.

[2]  L. Racioppi,et al.  Expression of major histocompatibility complex class I antigens in normal and transformed rat thyroid epithelial cell lines. , 1987, Cancer research.

[3]  R. Weinberg,et al.  N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma , 1986, Cell.

[4]  M. Simonsen,et al.  The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Miyazaki,et al.  Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[6]  A. Fusco,et al.  A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern , 1985, Journal of Virology.

[7]  R. Mertelsmann,et al.  Regulatory role of a monomorphic determinant of HLA Class I antigens in T cell proliferation. , 1985, Journal of immunology.

[8]  H. Burgert,et al.  An adenovirus type 2 glycoprotein blocks cell surface expression of human histocompatibility class I antigens , 1985, Cell.

[9]  A. Simeone,et al.  Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells , 1985, Nature.

[10]  D. Tse,et al.  Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells , 1984, The Journal of experimental medicine.

[11]  J. Seidman,et al.  Structure of wild-type and mutant mouse β 2-microglobulin genes , 1982, Cell.

[12]  R. di Lauro,et al.  In vitro synthesis of 300 oo M r rat thyroglobulin subunit , 1982, FEBS letters.

[13]  Fabre Jw,et al.  Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains. , 1981 .

[14]  P. Thomas,et al.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[15]  H. Coon,et al.  Culture of hormone-dependent functional epithelial cells from rat thyroids. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. Eb,et al.  Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells , 1983, Nature.

[17]  Alan F. Williams,et al.  Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus , 1982, European journal of immunology.

[18]  J. Fabre,et al.  Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains. , 1981, Transplantation proceedings.

[19]  G. Snell.  CHAPTER 11 – BIOCHEMICAL AND STRUCTURAL PROPERTIES OF THE CELL MEMBRANE LOCATED ALLOANTIGENS OF THE MAJOR HISTOCOMPATIBILITY COMPLEX* , 1976 .

[20]  In vitro synthesis of 300 000 M r rat thyroglobulin subunit , 2022 .