Osteoclastic resorption of biomimetic calcium phosphate coatings in vitro.

A new biomimetic method for coating metal implants enables the fast formation of dense and homogeneous calcium phosphate coatings. Titanium alloy (Ti6Al4V) disks were coated with a thin, carbonated, amorphous calcium phosphate (ACP) by immersion in a saturated solution of calcium, phosphate, magnesium, and carbonate. The ACP-coated disks then were processed further by incubation in calcium phosphate solutions to produce either crystalline carbonated apatite (CA) or octacalcium phosphate (OCP). The resorption behavior of these three biomimetic coatings was studied using osteoclast-enriched mouse bone-marrow cell cultures for 7 days. Cell-mediated degradation was observed for both carbonated apatite and octacalcium phosphate coatings. Numerous resorption lacunae characteristic of osteoclastic resorption were found on carbonated apatite after cell culture. The results showed that carbonated apatite coatings are resorbed by osteoclasts in a manner consistent with normal osteoclastic resorption. Osteoclasts also degraded the octacalcium phosphate coatings but not by classical pit formation.

[1]  Y. Doi,et al.  Osteoclastic responses to various calcium phosphates in cell cultures. , 1999, Journal of biomedical materials research.

[2]  M. Yamaguchi,et al.  Inhibitory effect of genistein on osteoclast-like cell formation in mouse marrow cultures. , 1999, Biochemical pharmacology.

[3]  C. V. van Blitterswijk,et al.  Biomimetic calcium phosphate coatings on Ti6AI4V: a crystal growth study of octacalcium phosphate and inhibition by Mg2+ and HCO3-. , 1999, Bone.

[4]  H. Blair.  How the osteoclast degrades bone , 1998, BioEssays : news and reviews in molecular, cellular and developmental biology.

[5]  G. Daculsi,et al.  Osteoclastic resorption of calcium phosphate ceramics with different hydroxyapatite/beta-tricalcium phosphate ratios. , 1997, Biomaterials.

[6]  D. Carnes,et al.  Development of an in vitro culture system for the study of osteoclast activity and function. , 1994, Journal of endodontics.

[7]  Milenko Markovic,et al.  Octacalcium phosphate. 3. Infrared and Raman vibrational spectra , 1993 .

[8]  P. Nijweide,et al.  Identification of osteopontin in isolated rabbit osteoclasts. , 1992, Biochemical and biophysical research communications.

[9]  G. H. Nancollas,et al.  Kinetics and mechanisms of octacalcium phosphate dissolution at 37.degree.C , 1992 .

[10]  S. Sakamoto,et al.  The effect of substrate composition and condition on resorption by isolated osteoclasts. , 1989, Bone and mineral.

[11]  J. J. Grote,et al.  Macropore tissue ingrowth: a quantitative and qualitative study on hydroxyapatite ceramic. , 1986, Biomaterials.

[12]  W. E. Brown,et al.  Octacalcium Phosphate and Hydroxyapatite: Crystal Structure of Octacalcium Phosphate , 1962, Nature.

[13]  C. Blitterswijk,et al.  Fast Formation of Biomimetic Ca-P Coatings on Ti6Al4V , 1999 .

[14]  C. V. van Blitterswijk,et al.  Osteoclastic resorption of calcium phosphates is potentiated in postosteogenic culture conditions. , 1994, Journal of biomedical materials research.

[15]  J. Davies,et al.  Resorption of sintered synthetic hydroxyapatite by osteoclasts in vitro. , 1993, Biomaterials.

[16]  D. Wood,et al.  Replacement of the rabbit medial meniscus with a polyester-carbon fibre bioprosthesis. , 1990, Biomaterials.