E3-ubiquitin ligase Nedd4 determines the fate of AID-associated RNA polymerase II in B cells

During B-lymphocyte class switch recombination, mutagenesis of the immunoglobulin (Ig) locus requires RNA polII-dependent targeting of the DNA mutator AID, yet the mechanism is unknown. Sun et al. now show that E3-ubiquitin ligase Nedd4 destabilizes AID-associated RNA polII at Ig switch regions. Loss of Nedd4 activity leads to RNA exosome substrate accumulation at AID target genes. This study links noncoding RNA processing following RNA polII pausing with AID regulation during class switch recombination.

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