CHEMICAL PROPERTIES OF PORCINE LEUKOCYTE LYSOSOMAL HYDROLASES

Specific activities, pH optima and activation energies were determined for some of the acid hydrolases found in various sedimentation fractions of sonicated porcine leukocytes and for hydrolases solubilized by n-butyl alcohol extractions of leukocytes. Lysosomal enzyme latency was demonstrated for these hydrolases by suspending sediments from differential contrifugation in 0.0125–0.25M sucrose solutions and releasing the enzymes. Activation energies of some hydrolases especially cathepsin D and β-glucuronidase were low suggesting that these acid hydrolases could function efficiently during low temperature aging of meat. pH optima are compared to those of similar enzymes from other sources.

[1]  J. W. Bird,et al.  Degradation of myofibrillar proteins by cathepsins B and D. , 1977, The Biochemical journal.

[2]  A. Bailey,et al.  Age-related changes in collagen: the identification of reducible lysine-carbohydrate condensation products. , 1972, Biochemical and biophysical research communications.

[3]  J. Dingle,et al.  Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown. , 1971, The Biochemical journal.

[4]  M. Stiles,et al.  Subcellular distribution of human leukocytic cathepsins. , 1968, Blood.

[5]  D. Romeo,et al.  Purification and properties of beef heart β-glucuronidase , 1968 .

[6]  L. Yam,et al.  CYTOCHEMICAL DEMONSTRATION OF β-GLUCURONIDASE ACTIVITY IN BLOOD AND BONE MARROW CELLS , 1967 .

[7]  A. Allison.  Lysosomes and disease. , 1967, Scientific American.

[8]  A. Barrett.  Lysosomal acid proteinase of rabbit liver. , 1967, The Biochemical journal.

[9]  A. Iodice,et al.  Separation of cathepsins A and D of skeletal muscle , 1966 .

[10]  F. C. Parrish,et al.  Physicochemical Properties and Partial Purification of Porcine Muscle Cathepsin , 1966 .

[11]  A. Tappel,et al.  ISOLATION OF RAT-LIVER LYSOSOMES AND THEIR GENERAL PROPERTIES. , 1964, Biochimica et biophysica acta.

[12]  J. G. Hirsch,et al.  ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT , 1963, The Journal of experimental medicine.

[13]  W. Valentine,et al.  Human Leucocyte Arylsulphatase Activity * † , 1962, British journal of haematology.

[14]  Z. Cohn,et al.  THE ISOLATION AND PROPERTIES OF THE SPECIFIC CYTOPLASMIC GRANULES OF RABBIT POLYMORPHONUCLEAR LEUCOCYTES , 1960, The Journal of experimental medicine.

[15]  D. M. Doty,et al.  Enzymes in Beef Muscle, Over-All Assay and Partial Purification Procedures for Proteolytic Enzymes in Beef Muscle , 1959 .

[16]  K. S. Dodgson,et al.  The assay of arylsulphatases A and B in human urine. , 1959, Clinica chimica acta; international journal of clinical chemistry.

[17]  M. Swendseid,et al.  Variations in nucleotidase activity of leukocytes; studies with leukemia patients. , 1952, The Journal of laboratory and clinical medicine.

[18]  E. Chargaff,et al.  Composition of the desoxypentose nucleic acids of four genera of sea-urchin. , 1952, The Journal of biological chemistry.

[19]  M. L. Anson,et al.  THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN , 1938, The Journal of general physiology.

[20]  R. Macy,et al.  Water-Soluble Flavor and Odor Precursors of Meat. 4. Influence of Cooking on Nucleosides and Bases of Beef Steaks and Roasts and Their Relationship to Flavor, Aroma and Juiciness , 1970 .

[21]  D. Hegner.  Isolierung und Enzymbestand von Granula aus polymorphkernigen Leukozyten des peripheren Rinderblutes , 1968 .

[22]  F. C. Parrish,et al.  ENZYME CHARACTERIZATION Physicochemical properties of bovine muscle particulate cathepsin , 1967 .

[23]  E. Buddecke,et al.  Untersuchungen zur Chemie der Arterienwand, XI. Nachweis, Reinigung und Eigenschaften einer Chondroitin-4-Sulfatase2 aus der Aorta des Rindes , 1967 .

[24]  E. Buddecke,et al.  Untersuchungen zur Chemie der Arterienwand, IX. Reinigung und Eigenschaften der β-Glucuronidase aus der Aorta des Rindes , 1966 .

[25]  B. Plapp,et al.  Purification and characterization of bovine liver beta-glucuronidase. , 1966, Archives of biochemistry and biophysics.

[26]  G. T. Mills,et al.  Studies on β-glucuronidase. 3. The influence of age, partial hepatectomy and other factors on the β-glucuronidase activity of rat liver , 1953 .

[27]  A. Roy.  The sulphatase of ox liver. I. The complex nature of the enzyme. , 1953, The Biochemical journal.