RESEARCH ARTICLE Studies of Metabolites in relation to Gonadal cycle of two fishes, Sillagosihama and Otolithus rubber at the Gulf of Kuchchh, India

Manuscript History: The investigation was carried out at the Southern coast of the Gulf of Kuchchh (22 o 41.6' N and 70 o 18.4; E). For the present study to fishes such as, Sillagosihama and Otolithus rubber were selected for detailed investigation. Sillagosihama and Otolithusruber were sacrificed in the field very quickly; the testes and ovaries of the fishes were dissected out separately from each of the two species for estimate the glycogen content. For samples of total lipid were brought to the laboratory and were oven dried at 48 o C for 3 to 5days and homogenized powders were made than dried samples were estimated by soxhlet apparatus using ethanol and petroleum ether mixture (3:1 ratio) as a solvent. The metabolites like Lipid in Ovary increase during the active process of gametogenesis in both the fish species. While the Glycogen level decreased in Sillagosihama, whereas in Otolithusruber, these metabolites showed an increased trend. This is due to as Sillagosihama laid eggs in batches, while Otolithusruber had a definite spawing time.