Confocal Raman microspectroscopy as a tool for studying the chemical heterogeneities of biofilms in situ

Aims:  To investigate the use of confocal Raman microspectroscopy (CRM) for the analysis of the structure, composition and development of fully hydrated biofilms.

[1]  T. R. Licht,et al.  Plasmid transfer in the animal intestine and other dynamic bacterial populations: the role of community structure and environment. , 1999, Microbiology.

[2]  D. Allison,et al.  Extracellular products as mediators of the formation and detachment of Pseudomonas fluorescens biofilms. , 1998, FEMS microbiology letters.

[3]  P. Suci,et al.  Action of Chlorhexidine Digluconate against Yeast and Filamentous Forms in an Early-Stage Candida albicans Biofilm , 2002, Antimicrobial Agents and Chemotherapy.

[4]  J. Costerton,et al.  Influence of Hydrodynamics and Cell Signaling on the Structure and Behavior of Pseudomonas aeruginosa Biofilms , 2002, Applied and Environmental Microbiology.

[5]  J Popp,et al.  Micro-Raman spectroscopic identification of bacterial cells of the genus Staphylococcus and dependence on their cultivation conditions. , 2005, The Analyst.

[6]  P. Stewart,et al.  Implications of reaction-diffusion theory for the disinfection of microbial biofilms by reactive antimicrobial agents , 1995 .

[7]  H. Bruining,et al.  Rapid Identification of Candida Species by Confocal Raman Microspectroscopy , 2002, Journal of Clinical Microbiology.

[8]  G. O’Toole,et al.  Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1 , 2003, Journal of bacteriology.

[9]  Stacie A. Brown,et al.  Membrane-Bound Nitrate Reductase Is Required for Anaerobic Growth in Cystic Fibrosis Sputum , 2007, Journal of bacteriology.

[10]  Shona Stewart,et al.  Studying Bacterial Metabolic States Using Raman Spectroscopy , 2006, Applied spectroscopy.

[11]  S. Molin,et al.  Alginate production affects Pseudomonas aeruginosa biofilm development and architecture, but is not essential for biofilm formation. , 2004, Journal of medical microbiology.

[12]  R. Kolter,et al.  Exopolysaccharide Production Is Required for Development of Escherichia coli K-12 Biofilm Architecture , 2000, Journal of bacteriology.

[13]  Peter Dawyndt,et al.  Raman microspectroscopy as an identification tool within the phylogenetically homogeneous 'Bacillus subtilis' group. , 2006, Systematic and applied microbiology.

[14]  J. Costerton,et al.  Antibiotic resistance of bacteria in biofilms , 2001, The Lancet.

[15]  P Stoodley,et al.  Survival strategies of infectious biofilms. , 2005, Trends in microbiology.

[16]  B. Lendl,et al.  Multidimensional information on the chemical composition of single bacterial cells by confocal Raman microspectroscopy. , 2000, Analytical chemistry.

[17]  E. Greenberg,et al.  Chelator-Induced Dispersal and Killing of Pseudomonas aeruginosa Cells in a Biofilm , 2006, Applied and Environmental Microbiology.

[18]  I. Sutherland,et al.  The biofilm matrix--an immobilized but dynamic microbial environment. , 2001, Trends in microbiology.

[19]  B. Christensen,et al.  Establishment of New Genetic Traits in a Microbial Biofilm Community , 1998, Applied and Environmental Microbiology.

[20]  D. Naumann,et al.  Prospective Study of the Performance of Vibrational Spectroscopies for Rapid Identification of Bacterial and Fungal Pathogens Recovered from Blood Cultures , 2003, Journal of Clinical Microbiology.

[21]  H. Bruining,et al.  Raman spectroscopic method for identification of clinically relevant microorganisms growing on solid culture medium. , 2000, Analytical chemistry.

[22]  T. Beveridge,et al.  Application of a pH-Sensitive Fluoroprobe (C-SNARF-4) for pH Microenvironment Analysis in Pseudomonas aeruginosa Biofilms , 2005, Applied and Environmental Microbiology.

[23]  N. A. Ngo Thi,et al.  Investigating the heterogeneity of cell growth in microbial colonies by FTIR microspectroscopy , 2007, Analytical and bioanalytical chemistry.

[24]  Ian P Thompson,et al.  Raman microscopic analysis of single microbial cells. , 2004, Analytical chemistry.

[25]  Matthew R. Parsek,et al.  Alginate is not a significant component of the extracellular polysaccharide matrix of PA14 and PAO1 Pseudomonas aeruginosa biofilms , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Kjelleberg,et al.  Bacteriophage and Phenotypic Variation in Pseudomonas aeruginosa Biofilm Development , 2004, Journal of bacteriology.

[27]  Z Lewandowski,et al.  Biofilms, the customized microniche , 1994, Journal of bacteriology.

[28]  P. Stewart,et al.  Direct measurement of chlorine penetration into biofilms during disinfection , 1994, Applied and environmental microbiology.

[29]  P. Suci,et al.  Integration of Raman microscopy, differential interference contrast microscopy, and attenuated total reflection Fourier transform infrared spectroscopy to investigate chlorhexidine spatial and temporal distribution in Candida albicans biofilms. , 2001, Journal of microbiological methods.

[30]  D. Naumann,et al.  Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study , 2001, Journal of Clinical Microbiology.

[31]  T. Beveridge,et al.  High-Resolution Visualization of Pseudomonas aeruginosa PAO1 Biofilms by Freeze-Substitution Transmission Electron Microscopy , 2005, Journal of bacteriology.

[32]  R. Pätzold,et al.  A new approach to non-destructive analysis of biofilms by confocal Raman microscopy , 2006, Analytical and bioanalytical chemistry.

[33]  S. Lane,et al.  Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy. , 2004, Analytical chemistry.

[34]  P. Wilderer,et al.  Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge , 2005, Applied and Environmental Microbiology.

[35]  P. Gemperline,et al.  Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy. , 2005, Analytical chemistry.

[36]  J. Costerton,et al.  Phenotypic differentiation and seeding dispersal in non-mucoid and mucoid Pseudomonas aeruginosa biofilms. , 2005, Microbiology.

[37]  Jürgen Popp,et al.  The identification of microorganisms by micro-Raman spectroscopy , 2003 .

[38]  S. Kjelleberg,et al.  A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms , 2006, Molecular microbiology.

[39]  Jürgen Popp,et al.  Raman spectroscopic identification of single yeast cells , 2005 .

[40]  Kees Maquelin,et al.  Rapid epidemiological analysis of Acinetobacter strains by Raman spectroscopy. , 2006, Journal of microbiological methods.

[41]  Michael Schmitt,et al.  Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room-Relevant Biological Contaminations , 2005, Applied and Environmental Microbiology.

[42]  Kyung-Min Yeon,et al.  Characterization of biofilm structure and its effect on membrane permeability in MBR for dye wastewater treatment. , 2006, Water research.

[43]  P. de Vos,et al.  Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species. , 2004, Analytical chemistry.

[44]  Hanqing Yu,et al.  Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila , 2005, Applied Microbiology and Biotechnology.

[45]  J. Wimpenny,et al.  Heterogeneity in biofilms. , 2000, FEMS microbiology reviews.

[46]  S. Wuertz,et al.  Microbial communities and their interactions in biofilm systems: an overview. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[47]  R. Norman,et al.  Effect of Pyocyanin on a Crude-Oil-Degrading Microbial Community , 2004, Applied and Environmental Microbiology.

[48]  E. Greenberg,et al.  Putative Exopolysaccharide Synthesis Genes Influence Pseudomonas aeruginosa Biofilm Development , 2004, Journal of bacteriology.

[49]  Cesar Mello,et al.  Rapid differentiation among bacteria that cause gastroenteritis by use of low-resolution Raman spectroscopy and PLS discriminant analysis , 2005, Analytical and bioanalytical chemistry.

[50]  I. Sutherland Biofilm exopolysaccharides: a strong and sticky framework. , 2001, Microbiology.

[51]  M. Lafleur,et al.  Characterization of the Diffusion of Polyethylene Glycol in Streptococcus Mutans Biofilms by Raman Microspectroscopy , 2004, Applied spectroscopy.

[52]  D. Naumann,et al.  Investigating Microbial (Micro)colony Heterogeneity by Vibrational Spectroscopy , 2001, Applied and Environmental Microbiology.