Electrophoretic separation on agarose thin film of isoenzymes of alkaline phosphatase from human serum and tissue.

An electrophoretic method is presented for separating alkaline phosphatase isoenzymes on agarose gel by using barbital buffer. α-Naphthyl AS-MX phosphate was converted to the highly fluorescent α-naphthol AS-MX, to detect the zones of enzymic activity on the thin film. The fluorescent zones on the electropherograms were scanned with a recording fluorometer and the electrophoretic mobilities of the enzymes were compared with those of the plasma proteins. This method was used to assess the isoenzyme patterns in human sera and tissues (liver, bone, kidney, spleen, and placenta).