Pumping of mammalian cells with a nozzle-diffuser micropump.

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.

[1]  E. Degerman,et al.  Airborne cell analysis. , 2000, Analytical chemistry.

[2]  T. Decker,et al.  A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. , 1988, Journal of immunological methods.

[3]  G. Dubyak.  Signal transduction by P2-purinergic receptors for extracellular ATP. , 1991, American journal of respiratory cell and molecular biology.

[4]  C. Korzeniewski,et al.  An enzyme-release assay for natural cytotoxicity. , 1983, Journal of immunological methods.

[5]  Nam-Trung Nguyen,et al.  MEMS-Micropumps: A Review , 2002 .

[6]  W Cris Wilson,et al.  Cell and organ printing 1: protein and cell printers. , 2003, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology.

[7]  M J Sanderson,et al.  Intercellular calcium signaling induced by extracellular adenosine 5'-triphosphate and mechanical stimulation in airway epithelial cells. , 1993, Journal of cell science.

[8]  Vladimir Mironov,et al.  Printing technology to produce living tissue , 2003, Expert opinion on biological therapy.

[9]  M. Gijs,et al.  A PMMA valveless micropump using electromagnetic actuation , 2005 .

[10]  Martin A. M. Gijs,et al.  Glass valveless micropump using electromagnetic actuation , 2005 .

[11]  Juan G. Santiago,et al.  A review of micropumps , 2004 .

[12]  Peter Enoksson,et al.  A Valve-Less Diffuser Micropump for Microfluidic Analytical Systems , 2001 .

[13]  I. Taniguchi,et al.  Novel disulfonated tetrazolium salt that can be reduced to a water-soluble formazan and its application to the assay of lactate dehydrogenase , 1995 .

[14]  G. Stemme,et al.  A valveless diffuser/nozzle-based fluid pump , 1993 .

[15]  Anders Olsson.  Valve-less diffuser micropumps , 1998 .

[16]  Urban Seger,et al.  Cell immersion and cell dipping in microfluidic devices. , 2004, Lab on a chip.

[17]  M. Ishiyama,et al.  A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye , 1993 .

[18]  Martin A. M. Gijs,et al.  Realization of hollow SiO2 micronozzles for electrical measurements on living cells , 2002 .