Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields

Nanosecond electric pulses (nsEPs) are known to cause a variety of effects on mammalian cells, ranging from destabilization of cell membranes to changes in cytoskeleton and elastic moduli. Measurement of a cells mechanoelastic properties have previously been limited to only invasive and destructive techniques such as atomic force microscopy or application of optical tweezers. However, due to recent advances, Brillouin spectroscopy has now become viable as a non-contact, non-invasive method for measuring these properties in cells and other materials. Here, we present progress toward applying Brillouin spectroscopy using a unique microscopy system for measuring changes in CHO-K1 cells when exposed to nsEPs of 600ns pulse duration with intensity of 50kV/cm. Successful measurement of mechanoelastic changes in these cells will demonstrate Brillouin spectroscopy as a viable method for measuring changes in elastic properties of other cells and living organisms.

[1]  William J. Polacheck,et al.  Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy , 2015, Nature Methods.

[2]  Gary L. Thompson,et al.  Role of cytoskeleton and elastic moduli in cellular response to nanosecond pulsed electric fields , 2013, Photonics West - Biomedical Optics.

[3]  Gerald J. Wilmink,et al.  Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines , 2011, PloS one.

[4]  Zachary A. Steelman,et al.  Cellular response to high pulse repetition rate nanosecond pulses varies with fluorescent marker identity. , 2016, Biochemical and biophysical research communications.

[5]  Michael R Murphy,et al.  Plasma membrane permeabilization by 60‐ and 600‐ns electric pulses is determined by the absorbed dose , 2009, Bioelectromagnetics.

[6]  S. Balevičius,et al.  Theoretical Analysis and Experimental Determination of the Relationships Between the Parameters of the Electric Field Pulse Required to Electroporate the Cells , 2013, IEEE Transactions on Plasma Science.

[7]  Marjorie A. Kuipers,et al.  Activation of intracellular phosphoinositide signaling after a single 600 nanosecond electric pulse. , 2013, Bioelectrochemistry.

[8]  Vladislav V. Yakovlev,et al.  Elasticity-based identification of tumor margins using Brillouin spectroscopy , 2016, SPIE BiOS.

[9]  Zhaokai Meng,et al.  Precise Determination of Brillouin Scattering Spectrum Using a Virtually Imaged Phase Array (VIPA) Spectrometer and Charge-Coupled Device (CCD) Camera , 2016, Applied spectroscopy.

[10]  Zhaokai Meng,et al.  Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis , 2015, Journal of biophotonics.

[11]  Gary L. Thompson,et al.  Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells , 2014, Apoptosis.

[12]  Marlan O Scully,et al.  Dual Raman-Brillouin Microscope for Chemical and Mechanical Characterization and Imaging. , 2015, Analytical chemistry.

[13]  Caleb C Roth,et al.  Resolving the spatial kinetics of electric pulse-induced ion release. , 2012, Biochemical and biophysical research communications.

[14]  Juergen F Kolb,et al.  Membrane permeabilization and cell damage by ultrashort electric field shocks. , 2007, Archives of biochemistry and biophysics.

[15]  Zhaokai Meng,et al.  Flow cytometry using Brillouin imaging and sensing via time-resolved optical (BISTRO) measurements. , 2015, The Analyst.

[16]  Gary L. Thompson,et al.  Disruption of the actin cortex contributes to susceptibility of mammalian cells to nanosecond pulsed electric fields. , 2014, Bioelectromagnetics.

[17]  Vladislav V. Yakovlev,et al.  Seeing cells in a new light: a renaissance of Brillouin spectroscopy , 2016 .

[18]  Zhaokai Meng,et al.  Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy , 2016, Journal of biophotonics.

[19]  Martin A Gundersen,et al.  Nanoelectropulse-driven membrane perturbation and small molecule permeabilization , 2006, BMC Cell Biology.

[20]  Vladislav V. Yakovlev,et al.  Background clean-up in Brillouin microspectroscopy of scattering medium. , 2014, Optics express.

[21]  Vladislav V. Yakovlev,et al.  Stimulated Brillouin Scattering Microscopic Imaging , 2015, Scientific Reports.

[22]  Jacob Yadegar,et al.  Dual-imaging system for burn depth diagnosis. , 2014, Burns : journal of the International Society for Burn Injuries.

[23]  K. Schoenbach,et al.  Sub-microsecond, intense pulsed electric field applications to cells show specificity of effects. , 2003, Bioelectrochemistry.

[24]  K. Schoenbach,et al.  Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition , 2001, PPPS-2001 Pulsed Power Plasma Science 2001. 28th IEEE International Conference on Plasma Science and 13th IEEE International Pulsed Power Conference. Digest of Papers (Cat. No.01CH37251).

[25]  K. Schoenbach,et al.  The effects of intense submicrosecond electrical pulses on cells. , 2003, Biophysical journal.

[26]  Vladislav V. Yakovlev,et al.  Impulsive Brillouin microscopy , 2017 .

[27]  Dustin G. Mixon,et al.  Plasma membrane permeabilization by trains of ultrashort electric pulses. , 2010, Bioelectrochemistry.

[28]  Vladislav V. Yakovlev,et al.  Optimizing signal collection efficiency of the VIPA-based Brillouin spectrometer , 2015 .