Anisakis simplex: stage-specific antigens recognized by mice

Abstract To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion–secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS–PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion–secretion and surface antigen preparations from thirdstage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion–secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis

[1]  F. Ubeira,et al.  Anisakis simplex: antigen recognition and antibody production in experimentally infected mice , 1993, Parasite immunology.

[2]  I. Kosugi,et al.  A case of ileus caused by a spiruroid nematode. , 1992, International journal for parasitology.

[3]  Kagei Noboru,et al.  A case of abdominal syndrome caused by the presence of a large number of Anisakis larvae. , 1992 .

[4]  N. Kagei,et al.  A case of abdominal syndrome caused by the presence of a large number of Anisakis larvae. , 1992, International journal for parasitology.

[5]  A. Prince,et al.  Onchocerca volvulus: biochemical and morphological characteristics of the surface of third- and fourth-stage larvae. , 1990, Experimental parasitology.

[6]  D. Grove,et al.  Strongyloides stercoralis: antigenic analysis of infective larvae and adult worms. , 1990, International journal for parasitology.

[7]  T. Deardorff,et al.  Anisakis simplex: histopathological changes in experimentally infected CBA/J mice. , 1990, Experimental parasitology.

[8]  M. Itoh,et al.  Stage–specific antigens of Hymenolepis microstoma recognized in BALB/c mice , 1989, Parasite immunology.

[9]  D. Grove,et al.  Western blot analysis of reactivity to larval and adult Strongyloides ratti antigens in mice , 1988, Parasite immunology.

[10]  K. Onitake,et al.  Changes in surface antigens of Hymenolepis nana during differentiation and maturation in mice , 1987, Journal of Helminthology.

[11]  M. Kennedy,et al.  Stage-specific secreted antigens of the parasitic larval stages of the nematode Ascaris. , 1986, Immunology.

[12]  M. Kliks.  Human anisakiasis: an update. , 1986, JAMA.

[13]  D. Pritchard,et al.  Antigen stripping from the nematode epicuticle using the cationic detergent cetyltrimethylammonium bromide (CTAB) , 1985, Parasite immunology.

[14]  V. Neuhoff,et al.  Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis , 1985 .

[15]  R. Parkhouse,et al.  Stage-specific antigens of Trichinella spiralis , 1984, Parasitology.

[16]  M. Kliks.  Anisakiasis in the western United States: four new case reports from California. , 1983, The American journal of tropical medicine and hygiene.

[17]  R. Parkhouse,et al.  Immune response to stage-specific surface antigens of the parasitic nematode Trichinella spiralis , 1981, The Journal of experimental medicine.

[18]  M. Kumada,et al.  Evaluation of somatic and "ES" antigens causing immunological injury of mast cells in mice infected with Anisakis larvae. , 1972, Japanese journal of medical science & biology.

[19]  D. Gibson.  Aspects of the development of 'herringworm' (Anisakis sp. larva) in experimentally infected rats. , 1970 .

[20]  P. C. Young,et al.  Larval nematodes from fish of the subfamily Anisakinae and gastro-intestinal lesions in mammals. , 1969, Journal of comparative pathology.

[21]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.