Phenotypic mixing: mechanism of dependent transmission for two related isolates of barley yellow dwarf virus

Hu, J. S., Rochow, W. F., Palukaitis, P., and Dietert, R. R. 1988. Phenotypic mixing: Mechanism of dependent transmission for two related isolates of barley yellow dwarf virus. Phytopathology 78:1326-1330. Preparations from oat plants singly or doubly infected with the MAV with antibodies specific for either virus and then tested in membraneand PAV isolates of barley yellow dwarf virus were used to study the feeding assays, the rate of MAV transmission by R. padi was reduced mechanism of dependent transmission of MAV by Rhopalosiphumpadi in significantly. These results demonstrated that some virions in the mixed the presence of the related PAV isolate. In two-site enzyme immunosorbent infection contain epitopes from both MAV and PAV and suggested that assays, virions trapped by PAV-specific polyclonal antibodies reacted with phenotypic mixing is a mechanism for dependent transmission of MAV MAV-specific monoclonal antibodies only in preparations made from together with PAV. doubly infected plants. When such virus preparations were precipitated Additional keywords: aphid vectors. Dependent transmission from mixed virus infections is a special transmission, aphids transmit one virus (dependent virus) only in feature of some aphid-virus relationships. In dependent the presence ofa second virus (helper virus). Previous studies of the mechanism of dependent transmission for persistent viruses This article is in the public domain and not copyrightable. It may be freely indicated that, during simultaneous replication of two viruses, reprinted with customary crediting of the source. The American some of the nucleic acid of the dependent virus becomes coated by Phytopathological Society, 1988. the protein capsid of the helper virus (21). Such "atypical" viruses