Semi-micro flow injection analysis method for evaluation of quenching effect of health foods or food additive antioxidants on peroxynitrite.

A semi-micro flow injection analysis (SMFIA) method for evaluation of quenching effect of food additive antioxidants or health foods on peroxynitrite (ONOO(-)) is described. The injected sample was carried with phosphate buffer containing NaNO(2), mixed with a trigger solution to generate ONOO(-) and then detected CL generated after mixing with luminol solution. Selective chemiluminescence caused by ONOO(-) in this generation system was confirmed by catalase treatment. Ascorbic acid (ASA), Trolox and ascorbyl palmitate (ASP) were used as food additive antioxidants. EC(50) values of ASA, Trolox and ASP were 2.6, 6.4 and 43 µg/mL, respectively. The amount of reagents required for an assay by this SMFIA system could reduce the time by a third compared with the conventional method previously reported. Furthermore, as an application of the proposed method, the quenching effect of commercially available Noni (Morinda citrifolia) juices was evaluated.

[1]  K. Nakashima,et al.  Quantification of coumarin derivatives in Noni (Morinda citrifolia) and their contribution of quenching effect on reactive oxygen species. , 2009 .

[2]  H. Tsuchiya,et al.  Antioxidant Activity Analysis by Liposomal Membrane System and Application to Anesthetics , 2008, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[3]  J. Nazaruk,et al.  Determination of phenolic compounds and their antioxidant activity in Erigeron acris L. extracts and pharmaceutical formulation by flow injection analysis with inhibited chemiluminescent detection. , 2008, Journal of pharmaceutical and biomedical analysis.

[4]  C. Georgiou,et al.  High throughput flow injection bioluminometric method for olive oil antioxidant capacity. , 2008, Food chemistry.

[5]  T. Bucciarelli,et al.  Protective effect of lycopene in cardiovascular disease. , 2008, European review for medical and pharmacological sciences.

[6]  D. V. van Thiel,et al.  Ultrasensitive peroxynitrite-based luminescence with L-012 as a screening system for antioxidative/antinitrating substances, e.g. Tylenol (acetaminophen), 4-OH tempol, quercetin and carboxy-PTIO. , 2007, Luminescence : the journal of biological and chemical luminescence.

[7]  K. Papadopoulos,et al.  Chemiluminescent studies on the antioxidant activity of amino acids. , 2007, Analytica chimica acta.

[8]  A. Vlessidis,et al.  On-line selective detection of antioxidants free-radical scavenging activity based on Co(II)/EDTA-induced luminol chemiluminescence by flow injection analysis. , 2007, Analytica chimica acta.

[9]  N. Kishikawa,et al.  Chemiluminescent screening of quenching effects of natural colorants against reactive oxygen species: Evaluation of grape seed, monascus, gardenia and red radish extracts as multi-functional food additives , 2007 .

[10]  O. Pechanova,et al.  The role of nitric oxide in the maintenance of vasoactive balance. , 2007, Physiological research.

[11]  N. Kuroda,et al.  Flow Injection Analysis with Luminol Chemiluminescence Detection for Evaluation of Quenching Effects of Grape Seed Extracts and Polyphenolics against Reactive Oxygen Species , 2006 .

[12]  H. Ohshima,et al.  Nitrative DNA damage in inflammation and its possible role in carcinogenesis. , 2006, Nitric oxide : biology and chemistry.

[13]  N. Kishikawa,et al.  Evaluation of quenching effects of non-water-soluble and water-soluble rosemary extracts against active oxygen species by chemiluminescent assay , 2004 .

[14]  V. Titov,et al.  Nitrite–Catalase Interaction as an Important Element of Nitrite Toxicity , 2003, Biochemistry (Moscow).

[15]  A. Townshend,et al.  Evaluation of the antioxidant effect of melatonin by flow injection analysis-luminol chemiluminescence. , 2003, Pharmacological research.

[16]  K. Dai,et al.  Dialysis membrane sampler for on-line flow injection analysis/chemiluminescence-detection of peroxynitrite in biological samples. , 2003, Talanta.

[17]  A. Saha,et al.  Determination of optimal conditions for synthesis of peroxynitrite by mixing acidified hydrogen peroxide with nitrite. , 1998, Free radical biology & medicine.