Toxoplasma gondii prevalence and risk factors in owned domestic cats from Nakhon Pathom Province, Thailand

Domestic cats are a potential source of Toxoplasma gondii infection for humans. This study was conducted to determine the seroprevalence and risk factors for T. gondii infection in domestic cats. Cat sera (n = 182) were tested for T. gondii IgG antibodies using the latex agglutination test (LAT) and the GRA7 of T. gondii (TgGRA7)-indirect enzyme-linked immunosorbent assay (iELISA). Univariable and multivariable logistic regression analyses were used to identify the factors associated with T. gondii infection. The overall prevalence rates were 18.1% (33/182) according to LAT, 19.2% (35/182) according to the iELISA and 17.0% (31/182) according to LAT and iELISA. Univariable analyses identified, outdoor access (P = 0.006), being a former stray cat (P = 0.001) and successful hunting behaviors (P = 0.04) as risk factors for T. gondii infection. Outdoor access (OR 2.63, 95% confidence interval (CI) 1.03–6.72) and is a former stray cat (OR 3.69, 95% CI 1.52–8.96) remained significant risk factors in multivariable analyses. This study indicated a relatively high seroprevalence of T. gondii among domestic cats. Cat owners can reduce the risk for T. gondii infection by not allowing their cats to roam free. Furthermore, education about the transmission of the parasite should be provided to prevent infection to the owners.

[1]  J. Cerón,et al.  Seroprevalence of Toxoplasma gondii in outdoor dogs and cats in Bangkok, Thailand , 2021, Parasitology.

[2]  Hla Myet Chel,et al.  Seroprevalence of Toxoplasma gondii in household cats in Myanmar and molecular identification of parasites using feline faecal oocysts , 2020, Food and waterborne parasitology.

[3]  Y. Nishikawa,et al.  Toxoplasma gondii and Neospora caninum prevalence and risk factors on goat farms in Kanchanaburi province, Thailand , 2020, Veterinary Integrative Sciences.

[4]  C. Vinke,et al.  A One Health Perspective on the Human–Companion Animal Relationship with Emphasis on Zoonotic Aspects , 2020, International journal of environmental research and public health.

[5]  C. Su,et al.  All about toxoplasmosis in cats: the last decade. , 2020, Veterinary parasitology.

[6]  Y. Nishikawa,et al.  Detection of antibodies against Toxoplasma gondii in cats using an immunochromatographic test based on GRA7 antigen , 2020, The Journal of veterinary medical science.

[7]  R. Noordin,et al.  Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals , 2019, European Journal of Clinical Microbiology & Infectious Diseases.

[8]  J. Dubey,et al.  Comparison of indirect and modified agglutination tests for detection of antibodies to Toxoplasma gondii in domestic cats , 2019, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[9]  Y. Nishikawa,et al.  Endemicity of Toxoplasma infection and its associated risk factors in Cebu, Philippines , 2019, PloS one.

[10]  F. Conraths,et al.  Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact , 2019, Food and waterborne parasitology.

[11]  I. Villena,et al.  Environmental transmission of Toxoplasma gondii: Oocysts in water, soil and food , 2019, Food and waterborne parasitology.

[12]  I. Navarro,et al.  Toxoplasma gondii: A study of oocyst re-shedding in domestic cats. , 2018, Veterinary parasitology.

[13]  H. Lohi,et al.  Toxoplasma gondii seroprevalence varies by cat breed , 2017, PloS one.

[14]  M. Igarashi,et al.  Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis. , 2017, Experimental parasitology.

[15]  G. Lin,et al.  Serological diagnosis of toxoplasmosis and standardization. , 2016, Clinica chimica acta; international journal of clinical chemistry.

[16]  M. AbouLaila,et al.  Seroprevalence and epidemiology of Toxoplasma gondii in farm animals in different regions of Egypt. , 2016, Veterinary parasitology, regional studies and reports.

[17]  M. A. O. Ignacio,et al.  How to cite this article , 2016 .

[18]  B. Lassen,et al.  Seroprevalence of and Risk Factors for Toxoplasma gondii Infection in Cats in Estonia. , 2015, Vector borne and zoonotic diseases.

[19]  Quan Liu,et al.  Diagnosis of toxoplasmosis and typing of Toxoplasma gondii , 2015, Parasites & Vectors.

[20]  Quan Liu,et al.  Evaluation of an Indirect Elisa Using Recombinant Granule Antigen Gra7 for Serodiagnosis of Toxoplasma gondii Infection in Cats , 2015, The Journal of parasitology.

[21]  L. Holec-Gąsior.  Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies , 2013, Clinical and Vaccine Immunology.

[22]  D. Jessup,et al.  Zoonotic Diseases Associated with Free‐Roaming Cats , 2013, Zoonoses and public health.

[23]  Y. Nishikawa,et al.  Development of an Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of Toxoplasma gondii Infection , 2013, Clinical and Vaccine Immunology.

[24]  A. Swart,et al.  Seroprevalence and risk factors for Toxoplasma gondii infection in domestic cats in The Netherlands. , 2012, Preventive veterinary medicine.

[25]  N. Rahmah,et al.  Recombinant proteins from new constructs of SAG1 and GRA7 sequences and their usefulness to detect acute toxoplasmosis. , 2012, Tropical biomedicine.

[26]  S. Jittapalapong,et al.  Epidemiology of Toxoplasma gondii infection of stray cats in Bangkok, Thailand. , 2010, The Southeast Asian journal of tropical medicine and public health.

[27]  P. Zhou,et al.  Seroprevalence of Toxoplasma gondii Infection in Stray and Household Cats in Guangzhou, China , 2009, Zoonoses and public health.

[28]  J P Dubey,et al.  History of the discovery of the life cycle of Toxoplasma gondii. , 2009, International journal for parasitology.

[29]  H. Kono,et al.  Seroprevalence of Toxoplasma gondii in cats and pigs from Thua Thien Hue Province in Vietnam. , 2009 .

[30]  M. Aslani,et al.  Seroprevalence of Toxoplasma gondii infection in stray and household cats in Tehran. , 2006, Veterinary parasitology.

[31]  J. Kaewkungwal,et al.  Toxoplasma gondii antibody in Thai cats and their owners. , 2003, The Southeast Asian journal of tropical medicine and public health.

[32]  M. Rohela,et al.  Toxoplasmosis: prevalence and risk factors , 2003, Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology.

[33]  A. Ortuño,et al.  Seroprevalence of Toxoplasma gondii Antibodies in Domestic Cats from Barcelona, Spain , 2003, The Journal of parasitology.

[34]  D. Hill,et al.  Toxoplasma gondii: transmission, diagnosis and prevention. , 2002, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[35]  K. Chokephaibulkit,et al.  Toxoplasma gondii antibodies in HIV and non-HIV infected Thai pregnant women. , 2001, Asian Pacific journal of allergy and immunology.

[36]  S. Maruyama,et al.  Seroprevalence of Bartonella henselae and Toxoplasma gondii among healthy individuals in Thailand. , 2000, The Journal of veterinary medical science.

[37]  M. Vercammen,et al.  Evaluation of Recombinant Dense Granule Antigen 7 (GRA7) of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies and Analysis of a Major Antigenic Domain , 1999, Clinical Diagnostic Laboratory Immunology.

[38]  S. Motoyoshi,et al.  Seroprevalence against Toxoplasma gondii in domiciled cats in Japan. , 1998, The Journal of veterinary medical science.

[39]  J. Dubey,et al.  Duration of immunity to shedding of Toxoplasma gondii oocysts by cats. , 1995, The Journal of parasitology.

[40]  J. P. Dubey,et al.  Reshedding of Toxoplasma oocysts by chronically infected cats , 1976, Nature.

[41]  Rustam,et al.  Toxoplasmosis in man and animals in South Kalimantan (Borneo), Indonesia. , 1976, The American journal of tropical medicine and hygiene.