Construction and characterization of Escherichia coli polA-lacZ gene fusions

The promoter of the polA gene of Escherichia coli K-12 was fused to the lacZ gene by selecting deletions within a lambda lacZ polA transducing phage. Four fusions, deleting varying amounts of the polA gene, were characterized. The polA promoter was found to be approximately 3% as active as the fully induced lac promoter. This figure is compatible with the normal intracellular level of deoxyribonucleic acid polymerase I. No evidence was found for outogenous regulation of transcription from the polA promoter. Expression from this promoter was influenced by neither recA nor mitomycin C, but uvrD and uvrE mutations reduced expression slightly.

[1]  N. Murray,et al.  Convergent transcription in bacteriophage λ: Interference with gene expression☆ , 1979 .

[2]  Brown,et al.  DNA Synthesis , 1978, NATO Advanced Study Institutes Series.

[3]  T. Gingeras,et al.  A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum. , 1978, Journal of molecular biology.

[4]  S. R. Kushner,et al.  UVRD, UVRE AND RECL REPRESENT A SINGLE GENE , 1978 .

[5]  R. Davies,et al.  Nucleotide sequence of the attachment site of coliphage lambda , 1977, Nature.

[6]  W. Ross,et al.  Viral integration and excision: structure of the lambda att sites. , 1977, Science.

[7]  S. G. Hughes.  A map of the cleavage sites for endonuclease AvaI in the chromosome of bacteriophage lambda. , 1977, Biochemical Journal.

[8]  W. J. Brammar,et al.  Characterization of λtrp-transducing bacteriophages made in vitro , 1976 .

[9]  S. G. Hughes,et al.  Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis. , 1976, The Biochemical journal.

[10]  I. Lehman,et al.  DNA polymerase I: essential replication enzyme. , 1976, Science.

[11]  M. Casadaban,et al.  Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. , 1976, Journal of molecular biology.

[12]  R. Burgess,et al.  Identification of two copies of the gene for the elongation factor EF–Tu in E. Coli , 1975, Nature.

[13]  W. Reznikoff,et al.  Genetic fusions defining trp and lac operon regulatory elements. , 1975, Journal of molecular biology.

[14]  M. Radman,et al.  SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. , 1975, Basic life sciences.

[15]  S. Cohen,et al.  Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic Acid , 1974, Journal of bacteriology.

[16]  W. Reznikoff,et al.  Inhibition of lacZ Gene Translation Initiation in trp-lac Fusion Strains , 1974, Journal of bacteriology.

[17]  E. Siegel.  Ultraviolet-Sensitive Mutator Strain of Escherichia coli K-12 , 1973, Journal of bacteriology.

[18]  C. Yanofsky,et al.  Tryptophan synthetase chain positions affected by mutations near the ends of the genetic map of trpA of Escherichia coli. , 1972, The Journal of biological chemistry.

[19]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[20]  J. Shapiro,et al.  Transposition of the lac Region to the gal Region of the Escherichia coli Chromosome: Isolation of λlac Transducing Bacteriophages , 1971, Journal of bacteriology.

[21]  E. Signer,et al.  The Role of Recombination in Growth of Bacteriophage Lambda II. Inhibition of Growth by Prophage P2 , 1971 .

[22]  Ronald W. Davis,et al.  [31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids , 1971 .

[23]  E. Signer,et al.  The Role of Recombination in Growth of Bacteriophage Lambda I. The Gamma Gene , 1971 .

[24]  A. D. Hershey,et al.  The Bacteriophage Lambda. , 1971 .

[25]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[26]  J. Cairns,et al.  Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase , 1969, Nature.

[27]  T. Jovin,et al.  Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. , 1969, The Journal of biological chemistry.

[28]  A. Kornberg.  Active center of DNA polymerase. , 1969, Science.

[29]  F. Jacob,et al.  Genetic mapping of the regulator and operator genes of the lac operon. , 1968, Journal of molecular biology.

[30]  E. Signer,et al.  Recombination in bacteriophage λ: II. Site-specific recombination promoted by the integration system , 1968 .

[31]  W. Gilbert,et al.  Mutants that make more lac repressor. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[32]  W. J. Brammar,et al.  Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of Escherichia coli. , 1968, Journal of molecular biology.

[33]  E. Adelberg,et al.  Map positions and specificities of suppressor mutations in Escherichia coli K-12. , 1965, Genetics.

[34]  I. Lehman,et al.  Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. , 1958, The Journal of biological chemistry.

[35]  R. Appleyard.  Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. , 1954, Genetics.