Ex vivo determination of glucose permeability and optical attenuation coefficient in normal and adenomatous human colon tissues using spectral domain optical coherence tomography

Abstract. Recent reports have suggested that spectral domain optical coherence tomography (SD-OCT) is a useful tool for quantifying the permeability of hyperosmotic agents in various tissues. We report our preliminary results on quantification of glucose diffusion and assessment of the optical attenuation change due to the diffusion of glucose in normal and adenomatous human colon tissues in vitro by using a SD-OCT and then calculated the permeability coefficients (PC) and optical attenuation coefficients (AC). The PC of a 30% aqueous solution of glucose was 3.37±0.23×10−6  cm/s in normal tissue and 5.65±0.16×10−6  cm/s in cancerous colon tissue. Optical AC in a normal colon ranged from 3.48±0.37 to 2.68±0.82  mm−1 and was significantly lower than those seen in the cancerous tissue (8.48±0.95 to 3.16±0.69  mm−1, p<0.05). The results suggest that quantitative measurements of using PC and AC from OCT images could be a potentially powerful method for colon cancer detection.

[1]  Chenyang Xu,et al.  Characterization of atherosclerosis plaques by measuring both backscattering and attenuation coefficients in optical coherence tomography. , 2008, Journal of biomedical optics.

[2]  Application of hyperosmotic agent to determine gastric cancer with optical coherence tomography ex vivo in mice. , 2009, Journal of biomedical optics.

[3]  Massoud Motamedi,et al.  Specificity of noninvasive blood glucose sensing using optical coherence tomography technique: a pilot study. , 2003, Physics in medicine and biology.

[4]  Dirk J. Faber,et al.  Measurement of the axial point spread function in scattering media using single-mode fiber-based optical coherence tomography , 2003 .

[5]  F. P. Bolin,et al.  Refractive index of some mammalian tissues using a fiber optic cladding method. , 1989, Applied optics.

[6]  S. Xie,et al.  In vivo quantification of propylene glycol, glucose and glycerol diffusion in human skin with optical coherence tomography , 2010 .

[7]  David Levitz,et al.  Determination of optical scattering properties of highly-scattering media in optical coherence tomography images. , 2004, Optics express.

[8]  Huajiang Wei,et al.  Quantification of glycerol diffusion in human normal and cancer breast tissues in vitro with optical coherence tomography , 2010 .

[9]  H. Yura,et al.  Analysis of optical coherence tomography systems based on the extended Huygens-Fresnel principle. , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.

[10]  Freek J. van der Meer,et al.  ORIGINAL ARTICLE , 2006 .

[11]  J. Duker,et al.  Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography. , 2005, Ophthalmology.

[12]  Tianheng Wang,et al.  Optical scattering coefficient estimated by optical coherence tomography correlates with collagen content in ovarian tissue. , 2011, Journal of biomedical optics.

[13]  V V Tuchin,et al.  Coherent optical techniques for the analysis of tissue structure and dynamics. , 1999, Journal of biomedical optics.

[14]  V Westphal,et al.  High-resolution endoscopic imaging of the GI tract: a comparative study of optical coherence tomography versus high-frequency catheter probe EUS. , 2001, Gastrointestinal endoscopy.

[15]  Kirill V. Larin,et al.  Quantification of glucose diffusion in arterial tissues by using optical coherence tomography , 2007 .

[16]  Valery V. Tuchin,et al.  Handbook of Coherent-Domain Optical Methods , 2013 .

[17]  S. Boppart,et al.  Optical Coherence Tomography: Feasibility for Basic Research and Image-guided Surgery of Breast Cancer , 2004, Breast Cancer Research and Treatment.

[18]  Valery V. Tuchin,et al.  Monte Carlo study of skin optical clearing to enhance light penetration in the tissue: implications for photodynamic therapy of acne vulgaris , 2007, Advanced Laser Technologies.

[19]  Dirk J. Faber,et al.  Localized measurement of optical attenuation coefficients of atherosclerotic plaque constituents by quantitative optical coherence tomography , 2005, IEEE Transactions on Medical Imaging.

[20]  V. Fuster,et al.  Water diffusion properties of human atherosclerosis and thrombosis measured by pulse field gradient nuclear magnetic resonance. , 1997, Arteriosclerosis, thrombosis, and vascular biology.

[21]  Valery V. Tuchin,et al.  Optical clearing of tissues and blood using the immersion method , 2005 .

[22]  Huajiang Wei,et al.  In vitro study of ultrasound and different-concentration glycerol-induced changes in human skin optical attenuation assessed with optical coherence tomography. , 2010, Journal of biomedical optics.

[23]  Samir Mitragotri,et al.  Dependence of Skin Permeability on Contact Area , 2003, Pharmaceutical Research.

[24]  S. Skinner,et al.  Microvascular structure of benign and malignant tumors of the colon in humans , 1995, Digestive Diseases and Sciences.

[25]  Ruikang K. Wang,et al.  Enhance light penetration in tissue for high-resolution optical imaging techniques by the use of biocompatible chemical agents , 2003, SPIE BiOS.

[26]  S. Xie,et al.  Depth-resolved monitoring of diffusion of hyperosmotic agents in normal and malignant human esophagus tissues using optical coherence tomography in-vitro , 2011 .

[27]  Adrian Glasser,et al.  Monitoring of glucose permeability in monkey skin in vivo using Optical Coherence Tomography , 2009, Journal of biophotonics.

[28]  Valery V Tuchin,et al.  Nondestructive quantification of analyte diffusion in cornea and sclera using optical coherence tomography. , 2007, Investigative ophthalmology & visual science.

[29]  S. Mitragotri,et al.  Ultrasound-Enhanced Drug Transport and Distribution in the Brain , 2010, AAPS PharmSciTech.

[30]  R. Esenaliev,et al.  Noninvasive blood glucose monitoring with optical coherence tomography: a pilot study in human subjects. , 2002, Diabetes care.

[31]  J W Pickering,et al.  Changes in the optical properties (at 632.8 nm) of slowly heated myocardium. , 1993, Applied optics.

[32]  Valery V Tuchin,et al.  Differential permeability rate and percent clearing of glucose in different regions in rabbit sclera. , 2008, Journal of biomedical optics.

[33]  R. Summers,et al.  Simultaneous morphology and molecular imaging of colon cancer , 2009, 2009 IEEE/NIH Life Science Systems and Applications Workshop.

[34]  Huajiang Wei,et al.  Quantifying glucose permeability and enhanced light penetration in ex vivo human normal and cancerous esophagus tissues with optical coherence tomography , 2010 .

[35]  Massoud Motamedi,et al.  Precision of measurement of tissue optical properties with optical coherence tomography. , 2003, Applied optics.

[36]  Alvin T Yeh,et al.  Molecular interactions of exogenous chemical agents with collagen--implications for tissue optical clearing. , 2006, Journal of biomedical optics.

[37]  Dirk Faber,et al.  Functional optical coherence tomography : spatially resolved measurements of optical properties , 2005 .

[38]  Valery V Tuchin,et al.  Depth-resolved monitoring of glucose diffusion in tissues by using optical coherence tomography. , 2006, Optics letters.

[39]  V V Tuchin,et al.  Light propagation in tissues with controlled optical properties , 1996, European Conference on Biomedical Optics.

[40]  J van Marle,et al.  Determination of the scattering anisotropy with optical coherence tomography. , 2011, Optics express.

[41]  M Motamedi,et al.  Noninvasive monitoring of glucose concentration with optical coherence tomography. , 2001, Optics letters.