Isoflavone-enriched soybean leaves attenuate ovariectomy-induced osteoporosis in rats by anti-inflammatory activity.

BACKGROUND with the aging population increases, postmenopausal osteoporosis has become a global public health problem. Previous evidence has shown that postmenopausal osteoporosis is a skeletal disease mainly caused by estrogen deficiency, generally accompanied by inflammation, and dietary isoflavones may ameliorate postmenopausal osteoporosis by anti-inflammatory activity. We have generated isoflavone-enriched soybean leaves (IESLs), but their anti-inflammatory activity and effect on attenuating osteoporosis are still obscure. Here, we determined the isoflavone profiles of IESLs, and evaluated their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and anti-osteoporotic effects on ovariectomy-induced osteoporisis in rats. RESULTS IESLs had high content of total isoflavone. Hydrolysate of IESLs (HIESLs) was rich with aglycones daidzein and genistein and HIESLs can significantly inhibit LPS-induced inflammation by reducing mRNA expression of iNOS, COX-2, IL6, and IL1β. Moreover, ovariectomized rats receiving aqueous extracts of IESLs (HIESLs) orally maintained more bone mass than control rats, which was attributed to inhibition of osteoclastogenesis by down-regulating the mRNA expression of the bone-specific genes RANKL/OPG, OC, and cathepsin K, and the inflammation-related genes IL6, NFκB, and COX-2. CONCLUSION IESLs may attenuate postmenopausal osteoporosis by suppressing osteoclastogenesis with anti-inflammatory activity, and be a potential source of functional food ingredients for the prevention of osteoporosis. This article is protected by copyright. All rights reserved.

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