Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum

To identify bacterial traits related to adhesion ability in human bifidobacteria, 13 strains of Bifidobacterium longum isolated from human gastric juice and intestine were studied. Strains were tested for their capability to adhere to Caco‐2 cells and classified as adhesive (Adh+) or non‐adhesive (Adh–). Adh+ and Adh– strains were then investigated for their autoaggregation ability and surface hydrophobicity. Comparing the properties of Adh+ and Adh–, we observed that strains were able to adhere to cell monolayers if they autoaggregate and manifest a good degree of hydrophobicity as determined by microbial adhesion to hydrocarbons. These two traits could be used for preliminary screening to identify potentially adherent isolates.

[1]  J. Forstner,et al.  Purification and characterization of a novel protein produced by Bifidobacterium longum SBT2928 that inhibits the binding of enterotoxigenic Escherichia coli Pb176 (CFA/II) to gangliotetraosylceramide , 1999, Journal of applied microbiology.

[2]  D. Brassart,et al.  Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsoniiLa1 to Human Enterocyte-Like Caco-2 Cells , 1999, Applied and Environmental Microbiology.

[3]  G. Vignola,et al.  Autoaggregation and adhesion ability in a Bifidobacterium suis strain , 1998, Letters in applied microbiology.

[4]  P. Pérez,et al.  Surface Properties of Bifidobacterial Strains of Human Origin , 1998, Applied and Environmental Microbiology.

[5]  D. Brassart,et al.  Immune modulation of blood leukocytes in humans by lactic acid bacteria: criteria for strain selection. , 1997, The American journal of clinical nutrition.

[6]  R. Korpela,et al.  Recovery of Lactobacillus rhamnosus GG from human colonic biopsies. , 1996, Letters in applied microbiology.

[7]  K. Tahri,et al.  Effects of three strains of bifidobacteria on cholesterol , 1995, Letters in applied microbiology.

[8]  J. Grill,et al.  Adhesion of different bifidobacteria strains to human enterocyte‐like Caco‐2 cells and comparison with in vivo study , 1995, Letters in applied microbiology.

[9]  G. Gibson,et al.  Regulatory effects of bifidobacteria on the growth of other colonic bacteria. , 1994, The Journal of applied bacteriology.

[10]  D. Brassart,et al.  Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions , 1993, Applied and environmental microbiology.

[11]  S. Ahrné,et al.  Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora , 1993, Applied and environmental microbiology.

[12]  B. Biavati,et al.  Bifidobacterium saeculare: a New Species Isolated from Feces of Rabbit , 1991 .

[13]  Z. Policova,et al.  In vitro surface properties of the newly recognized gastric pathogen Helicobacter pylori , 1990, Infection and immunity.

[14]  L. Axelsson,et al.  Surface properties of lactobacilli isolated from the small intestine of pigs. , 1987, The Journal of applied bacteriology.

[15]  J. Olsson,et al.  Hydrophobicity and adherence of oral streptococci after repeated subculture in vitro , 1983, Infection and immunity.

[16]  S. Hjertén,et al.  A new test based on ‘salting out’ to measure relative hydrophobicity of bacterial cells , 1981 .

[17]  T. Orfeo,et al.  One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. , 1977, Journal of the National Cancer Institute.

[18]  B. Biavati,et al.  The genus Bifidobacterium , 1995 .

[19]  H. C. van der Mei,et al.  Use of flow chamber devices and image analysis methods to study microbial adhesion. , 1995, Methods in enzymology.

[20]  S. Hjertén,et al.  A new test based on 'salting out' to measure relative surface hydrophobicity of bacterial cells. , 1981, Biochimica et biophysica acta.