In vitro assay of cytotoxicity with cultured liver: accomplishments and possibilities.

Tissue cultures offer potential advantages for assaying the toxicity of chemicals and for evaluating tissue susceptibility to toxic agents. Several properties of cultured cells hinder the immediate, widespread use of tissue cultures to assay toxicity routinely. These points are illustrated by briefly reviewing attempts to utilize different types of hepatic cultures to evaluate the actions of carcinogenic chemicals in vitro. Hepatocytes in vivo apparently can metabolize all known procarcinogenic chemicals, but the process of tissue isolation and the environmental conditions in vitro may modify drastically the responses of hepatocytes and other cultured hepatic cells to toxic chemicals. Before cell cultures can be used routinely as the basis of screening systems to detect chemical toxins, specificity and sensitivity of response to chemicals representing all chemical classes must be validated by laboratory studies.

[1]  J A Miller,et al.  DNA repair in primary cultures of rat hepatocytes. , 1978, Cancer research.

[2]  S. Orrenius,et al.  Metabolism of benzo(a)pyrene with isolated hepatocytes and the formation and degradation of DNA-binding derivatives. , 1977, The Journal of biological chemistry.

[3]  D. Hsieh,et al.  Maintenance of cytochrome P-450 and metabolism of aflatoxin B1 in primary hepatocyte cultures. , 1977, Biochemical and biophysical research communications.

[4]  G. Williams,et al.  Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures. , 1977, Cancer research.

[5]  R. Boorstein,et al.  Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures , 1977, Nature.

[6]  G. B. Gori,et al.  Contribution of the environment to cancer incidence: an epidemiologic exercise. , 1977, Journal of the National Cancer Institute.

[7]  M. J. Phillips,et al.  Isolated mammalian hepatocytes in culture. , 1976, Gastroenterology.

[8]  H C Pitot,et al.  Interaction of chemical carcinogens and drug-metabolizing enzymes in primary cultures of hepatic cells from the rat. , 1976, The American journal of pathology.

[9]  G. Michalopoulos,et al.  Cytochrome P-450 induction by phenobarbital and 3-methylcholanthrene in primary cultures of hepatocytes. , 1976, Science.

[10]  P. Guzelian,et al.  Effect of cobalt on synthesis of heme and cytochrome P-450 in the liver. Studies of adult rat hepatocytes in primary monolayer culture and in vivo. , 1976, The Journal of biological chemistry.

[11]  Gary M Williams,et al.  Chapter 31 Primary and Long-Term Culture of Adult Rat Liver Epithelial Cells , 1976 .

[12]  Bissell Dm Study of hepatocyte function in cell culture. , 1976 .

[13]  G. Williams,et al.  Carcinogen-induced DNA repair in primary rat liver cell cultures; a possible screen for chemical carcinogens. , 1976, Cancer letters.

[14]  G. Williams Primary and long-term culture of adult rat liver epithelial cells. , 1976, Methods in Cell Biology.

[15]  D. Bissell Study of hepatocyte function in cell culture. , 1976, Progress in liver diseases.

[16]  G. Williams The use of liver epithelial cultures for the study of chemical carcinogenesis. , 1976, The American journal of pathology.

[17]  A. Lehmann,et al.  A comparison of the 8-azaguanine and ouabain-resistance systems for the selection of induced mutant Chinese hamster cells. , 1975, Mutation research.

[18]  P. Perry,et al.  Cytological detection of mutagen–carcinogen exposure by sister chromatid exchange , 1975, Nature.

[19]  C. Drevon,et al.  Production of epithelial and mesenchymal tumours with rat liver cells transformed in vitro , 1975, International journal of cancer.

[20]  W. Schaeffer,et al.  A diploid rat liver cell culture. III. Characterization of the heteroploid morphological variants which develop with time in culture. , 1975, Experimental cell research.

[21]  Grisham Jw,et al.  Insulin and hydrocortisone effects on viability and glycogen stores of postnatal rat liver organ culture. , 1975 .

[22]  G. Michalopoulos,et al.  Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations. , 1975, Experimental cell research.

[23]  P. Higgins,et al.  Properties and malignant transformation of established rat liver parenchymal cells in culture. , 1975, Journal of the National Cancer Institute.

[24]  K. Jeejeebhoy,et al.  Isolated rat hepatocytes as a model to study drug metabolism: dose-dependent metabolism of diphenylhydantoin. , 1975, Life sciences.

[25]  B. Ames,et al.  Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[26]  H. Zimmerman,et al.  Toxicity of tricyclic antidepressants to isolated rat hepatocytes. , 1975, Biochemical pharmacology.

[27]  J. Desgrès,et al.  Long term cell culture of rat liver epithelial cells retaining some hepatic functions. , 1975, Biochimie.

[28]  H. Stich,et al.  The use of DNA repair in the identification of carcinogens, precarcinogens, and target tissue , 1974 .

[29]  D Brusick,et al.  Comparison of the genetic activity of dimethylnitrosamine, ethyl methanesulfonate, 2-acetylaminofluorene and ICR-170 in Saccharomyces cerevisiae strains D3, D4 and D5 using in vitro assays with and without metabolic activation. , 1974, Mutation research.

[30]  R. Setlow,et al.  Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens. , 1974, Cancer research.

[31]  G. Greenberg,et al.  Ultrastructural and functional studies of cultured hepatocytes. , 1974, Laboratory investigation; a journal of technical methods and pathology.

[32]  H. Nakabayashi,et al.  Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells. , 1974, Acta medica Okayama.

[33]  A. Salmon,et al.  Benzo(a)pyrene Effects on Mouse Epithelial Cells in Culture , 1974 .

[34]  J. Whitlock,et al.  Aryl hydrocarbon (benzo(a)pyrene) hydroxylase induction in rat liver cells in culture. , 1974, The Journal of biological chemistry.

[35]  J. Rhim,et al.  Induction of hepatocarcinoma in vivo with fetal mouse liver cells spontaneously transformed in culture and isolation of a type C RNA virus from the carcinoma cells. , 1974, Cancer research.

[36]  U. Meyer,et al.  Parenchymal cells from adult rat liver in nonproliferating monolayer culture. I. Functional studies. , 1973, The Journal of cell biology.

[37]  P. Seglen Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion. , 1973, Experimental cell research.

[38]  U. Meyer,et al.  PARENCHYMAL CELLS FROM ADULT RAT LIVER IN NONPROLIFERATING MONOLAYER CULTURE , 1973, The Journal of cell biology.

[39]  M. Sporn,et al.  Localization of benzo(a)pyrene-3H and alterations in nuclear chromatin caused by benzo(a)pyrene-ferric oxide in the hamster respiratory epithelium. , 1973, Cancer Research.

[40]  Y. Tashiro,et al.  The WIRL-3 rat liver cell lines and their transformed derivatives. , 1973, Cancer research.

[41]  A. Schwartz The protective effect of estradiol-17 beta against polycyclic hydrocarbon cytotoxicity. , 1973, Cancer research.

[42]  L. Tomatis,et al.  Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine , 1973, British Journal of Cancer.

[43]  E. Bresnick,et al.  Induction of benzpyrene hydroxylase in fetal liver explants by flavones and phenobarbital. , 1973, Biochemical pharmacology.

[44]  G. Williams,et al.  Carcinoma after malignant conversion in vitro of epithelial-like cells from rat liver following exposure to chemical carcinogens. , 1973, Cancer research.

[45]  W. Glinsmann,et al.  Effect of insulin on glycogen synthesis in fetal rat liver in organ culture. , 1973, Endocrinology.

[46]  T. Takaoka,et al.  Carcinogenesis in tissue culture. XIV. Malignant transformation of rat liver parenchymal cells treated with 4-nitroquinoline 1-oxide in tissue culture. , 1972, Journal of the National Cancer Institute.

[47]  W. Benedict Early changes in chromosomal number and structure after treatment of fetal hamster cultures with transforming doses of polycyclic hydrocarbons. , 1972, Journal of the National Cancer Institute.

[48]  C. Monder,et al.  Influence of cortisol on glycogen synthesis and gluconeogenesis in fetal rat liver in organ culture. , 1972, The Journal of biological chemistry.

[49]  A. Zuckerman,et al.  Induction of morphological changes in human embryo liver cells by the pyrrolizidine alkaloid lasiocarpine. , 1972, British journal of experimental pathology.

[50]  J. DiPaolo,et al.  Carcinomas from rat liver cells transformed spontaneously in culture. , 1972, Cancer research.

[51]  A. Zuckerman,et al.  The effects of lasiocarpine, retrorsine and retronecine pyrrole on human embryo lung and liver cells in culture. , 1972, British journal of experimental pathology.

[52]  C. Borek Neoplastic transformation in vitro of a clone of adult liver epithelial cells into differentiated hepatoma-like cells under conditions of nutritional stress. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[53]  H. Leffert,et al.  STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS , 1972, The Journal of cell biology.

[54]  W. A. Susor,et al.  Morphological and biochemical observations on mammalian liver cells in culture. Isolation of a clonal strain from rat liver. , 1972, Biochimie.

[55]  G. Williams,et al.  Isolation and long-term cell culture of epithelial-like cells from rat liver. , 1971, Experimental cell research.

[56]  Kaighn Me,et al.  Production of Albumin and Other Serum Proteins by Clonal Cultures of Normal Human Liver , 1971 .

[57]  P. Iype Cultures from adult rat liver cells I. Establishment of monolayer cell‐cultures from normal liver , 1971, Journal of cellular physiology.

[58]  D. Nebert,et al.  Aryl hydrocarbon hydroxylase induction in mammalian liver cell culture. II. Effects of actinomycin D and cycloheximide on induction processes by phenobarbital or polycyclic hydrocarbons. , 1971, The Journal of biological chemistry.

[59]  C. Hales,et al.  Maintenance of viable cells in an organ culture of mature rat liver. , 1971, Experimental cell research.

[60]  M. Kaighn,et al.  Production of albumin and other serum proteins by clonal cultures of normal human liver. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[61]  N. Ito,et al.  In vitro malignant transformation of cells derived from rat liver by means of aflatoxin B1. , 1970, Gan.

[62]  L. Gerschenson,et al.  Tyrosine Transaminase Induction by Dexamethasone in a New Rat Liver Cell Line , 1970, Science.

[63]  Rainer Laufs,et al.  Metabolic and Virologic Studies in Primate Liver Organ Cultures 1 , 1970, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[64]  J. Grisham,et al.  Explant culture of rat liver. I. Method, morphology, and cytogenesis. , 1970, Laboratory investigation; a journal of technical methods and pathology.

[65]  A. Zuckerman,et al.  The effect of heliotrine, a pyrrolizidine alkaloid, on human liver cells in culture. , 1969, British journal of experimental pathology.

[66]  J. DiPaolo,et al.  Quantitative studies of in vitro transformation by chemical carcinogens. , 1969, Journal of the National Cancer Institute.

[67]  R. Schindler Use of cell culture in pharmacology. , 1969, Annual review of pharmacology.

[68]  W. Loewenstein,et al.  Intercellular communication and tissue growth : IV. Conductance of membrane junctions of normal and cancerous cells in culture. , 1969, The Journal of membrane biology.

[69]  J. Higginson Present trends in cancer epidemiology. , 1969, Proceedings. Canadian Cancer Conference.

[70]  L. Gerschenson,et al.  Metabolism of rat liver cells cultured in suspension: insulin and glucagon effects on glycogen level. , 1968, Biochemical and biophysical research communications.

[71]  M. Kumegawa,et al.  THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS , 1968, The Journal of cell biology.

[72]  J. Melnick,et al.  Marmoset liver organ culture infected with herpes virus. , 1968, Experimental and molecular pathology (Print).

[73]  M. Namba,et al.  Carcinogenesis in tissue culture. 8. Spontaneous malignant transformation of rat liver cells in long-term culture. , 1968, The Japanese journal of experimental medicine.

[74]  A. Zuckerman,et al.  The effects of aflatoxins on human embryo liver cells in culture. , 1968, British journal of experimental pathology.

[75]  A. Zuckerman,et al.  The effect of tetracycline on human liver cells in culture. , 1968, British journal of experimental pathology.

[76]  W. F. Dryden,et al.  Tissue culture in the study of the effects of drugs. , 1967, Journal of pharmaceutical sciences.

[77]  K. Tsiquaye,et al.  Tissue culture of human embryo liver cells and the cytotoxicity of aflatoxin B1. , 1967, British journal of experimental pathology.

[78]  B. Sandstroem A METHOD OF CULTIVATING MORPHOLOGICALLY HIGHLY DIFFERENTIATED LIVER EPITHELIUM IN VITRO. , 1964, Acta Societatis Medicorum Upsaliensis.