Exhaled metallic elements and serum pneumoproteins in asymptomatic smokers and patients with COPD or asthma.

STUDY OBJECTIVES The aim of this study was to characterize the elemental composition of exhaled breath condensate (EBC) in order to identify new biomarkers of exposure and susceptibility in COPD patients. Serum pneumoproteins were used as lung-specific biomarkers of effect. DESIGN EBC was obtained from 50 healthy subjects, 30 healthy smokers, 30 asthmatics, and 50 patients with stable COPD, and was collected by cooling exhaled air. Trace elements and toxic metals in the samples were measured by means of inductively coupled plasma-mass spectrometry and electrothermal atomic absorption spectroscopy. The serum pneumoproteins were immunoassayed. RESULTS The EBC of COPD subjects had higher levels of such toxic elements as lead, cadmium, and aluminum, and lower levels of iron and copper, than that of the nonsmoking control subjects. There were no between-group differences in surfactant protein (SP)-A and SP-B levels. Clara-cell protein and SP-D levels were negatively and positively influenced, respectively, by tobacco smoke. CONCLUSIONS Our results show that toxic metals and transition elements are detectable in the EBC of studied subjects. We propose new biomarkers of exposure as a means of assessing the target tissue dose of carcinogenic and pneumotoxic substances from tobacco smoke or polluted workplaces, and the use of the transition elements involved in redox systems of oxidative stress as disease biomarkers associated with effect or susceptibility. Together with biomarkers of effect, such as serum pneumoproteins, the elemental composition of EBC may be clinically useful in distinguishing similar diseases.

[1]  Jennifer Su,et al.  Epithelial lining fluid solute concentrations in chronic obstructive lung disease patients and normal subjects. , 2005, Journal of applied physiology.

[2]  P. J. Barnes,et al.  Exhaled breath condensate: methodological recommendations and unresolved questions , 2005, European Respiratory Journal.

[3]  M. Corradi,et al.  Heart rate variability in non-apneic snorers and controls before and after continuous positive airway pressure , 2005, BMC Pulmonary Medicine.

[4]  P. Apostoli,et al.  Exhaled Breath Condensate as a Suitable Matrix to Assess Lung Dose and Effects in Workers Exposed to Cobalt and Tungsten , 2004, Environmental health perspectives.

[5]  I. Rahman,et al.  Non-invasive biomarkers of oxidative stress: reproducibility and methodological issues , 2004, Redox report : communications in free radical research.

[6]  U. Holmskov,et al.  Surfactant protein D in the female genital tract. , 2004, Molecular human reproduction.

[7]  F. Karadağ,et al.  Trace elements as a component of oxidative stress in COPD , 2004, Respirology.

[8]  D. Hendrick.  Smoking, cadmium, and emphysema , 2004, Thorax.

[9]  D. Mannino,et al.  Urinary cadmium levels predict lower lung function in current and former smokers: data from the Third National Health and Nutrition Examination Survey , 2004, Thorax.

[10]  M. Jadoul,et al.  Determinants of serum levels of surfactant proteins A and B and Clara cell protein CC16 , 2003, Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals.

[11]  G. Dusting,et al.  Superoxide dismutase mimetic M40403 improves endothelial function in apolipoprotein(E)‐deficient mice , 2003, British journal of pharmacology.

[12]  W. MacNee,et al.  Mechanism of lung injury caused by PM10 and ultrafine particles with special reference to COPD , 2003, European Respiratory Journal.

[13]  P. Apostoli.  Elements in environmental and occupational medicine. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[14]  M. Richard,et al.  Toxicity of Cadmium in Tobacco Smoke: Protection by Antioxidants and Chelating Resins , 2002, Free radical research.

[15]  H. Haussmann,et al.  Evaluation of the potential effects of ingredients added to cigarettes. Part 2: chemical composition of mainstream smoke. , 2002, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[16]  Z. Kováčiková,et al.  Comparative in vitro toxicity of cadmium and lead on redox cycling in type II pneumocytes , 2001, Journal of applied toxicology : JAT.

[17]  K. Garey,et al.  Collection and analysis of exhaled breath condensate in humans. , 2001, American journal of respiratory and critical care medicine.

[18]  L Battolla,et al.  Relationship between extent of pulmonary emphysema by high-resolution computed tomography and lung elastic recoil in patients with chronic obstructive pulmonary disease. , 2001, American journal of respiratory and critical care medicine.

[19]  P. Barnes,et al.  Exhaled markers of pulmonary disease. , 2001, American journal of respiratory and critical care medicine.

[20]  L. Fabbri,et al.  Cellular and structural bases of chronic obstructive pulmonary disease. , 2001, American journal of respiratory and critical care medicine.

[21]  R. Pauwels,et al.  Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary. , 2001, American journal of respiratory and critical care medicine.

[22]  U. Majewska,et al.  Trace element load in cancer and normal lung tissue , 1999 .

[23]  C Hermans,et al.  Lung epithelium-specific proteins: characteristics and potential applications as markers. , 1999, American journal of respiratory and critical care medicine.

[24]  D. Dockery,et al.  Epidemiology of Particle Effects , 1999 .

[25]  C. Hermans,et al.  Clearance of Clara cell secretory protein 16 (CC16) and surfactant proteins A and B from blood in acute respiratory failure. , 1998, American journal of respiratory and critical care medicine.

[26]  C. Hermans,et al.  Determinants of Clara cell protein (CC16) concentration in serum: a reassessment with two different immunoassays. , 1998, Clinica chimica acta; international journal of clinical chemistry.

[27]  S. Abe,et al.  Enzyme-linked immunosorbent assay using F(ab')2 fragment for the detection of human pulmonary surfactant protein D in sera. , 1997, Clinica chimica acta; international journal of clinical chemistry.

[28]  R. Pellegrino,et al.  On the causes of lung hyperinflation during bronchoconstriction. , 1997, The European respiratory journal.

[29]  S. Porru,et al.  Multiple determination of elements in human seminal plasma and spermatozoa. , 1997, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[30]  Y. Itokawa,et al.  Mechanism of oxidative stress in skeletal muscle atrophied by immobilization. , 1993, The American journal of physiology.

[31]  A. Bernard,et al.  Decrease of serum Clara cell protein in smokers , 1992, The Lancet.

[32]  M. Graziani,et al.  Tentative reference values for some elements in broncho-alveolar lavage fluid. , 1992, The Science of the total environment.

[33]  M. Chiba,et al.  Toxic and trace elements in tobacco and tobacco smoke. , 1992, Bulletin of the World Health Organization.

[34]  Philip J. Landrigan,et al.  Biological markers in environmental health research , 1987 .

[35]  R. Heimburger,et al.  Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy dispersive x-ray fluorescence. 1: Technique and determination of the normal reference interval. , 1985, Clinical chemistry.