RIPENING OF CLIMACTERIC FRUITS AND THEIR CONTROL

Ripening is the final stage of development of a fruit, which involves series of physiological and biochemical events that make them both attractive and tasty to eat. Phytohormones play an important role in fruit ripening. Treatment with ethylene is required for normal fruit ripening of climacteric fruits. The fruit ripening can be controlled or delayed by physical methods such as modified atmosphere by packaging, treatment with gamma irridiation, surface coating with sucrose ester, etc. The treatments with phytohormones, reducing agents and 1-methylenecyclopropene have also been successful in delaying fruit ripening. Molecular biology tools such as transgenic plants with suppression of genes involved in ethylene synthesis or overexpression of genes for ethylene degradation, virus-induced gene silencing and manipulation of transcription factors have been successfully utilized for controlling or regulating fruits ripening. PRACTICAL APPLICATIONS Fruits play a vital role in human nutrition. One of the limited factors that influence their economic value is the relatively short ripening period and reduced postharvest life. Overripening of fruits leads to excessive softening which results in spoilage and damage during shipping and handling. Slowing the process of ripening and senescence extends the storage and shelf life of fresh fruits. Increment in shelf life of fruits not only helps the grower to save on postharvest losses but consumers are also benefited in terms of retained freshness of fruit for longer period and thus results in value addition to the fruit.

[1]  A. Carrillo‐López,et al.  RIPENING AND QUALITY CHANGES IN MANGO FRUIT AS AFFECTED BY COATING WITH AN EDIBLE FILM , 2000 .

[2]  C. Sanz Quality improvement through postharvest management , 2005 .

[3]  C. Watkins,et al.  Inhibition of pear fruit ripening by mannose. , 1987, Plant physiology.

[4]  D. K. Tandon,et al.  Changes in postharvest quality of mangoes affected by preharvest application of calcium salts , 1993 .

[5]  R. Paull,et al.  Papaya fruit softening, endoxylanase gene expression, protein and activity. , 2007, Physiologia plantarum.

[6]  Pech,et al.  Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripening-related genes isolated by differential display , 1999, The Plant journal : for cell and molecular biology.

[7]  J. Ecker,et al.  Activation of the Ethylene Gas Response Pathway in Arabidopsis by the Nuclear Protein ETHYLENE-INSENSITIVE3 and Related Proteins , 1997, Cell.

[8]  J. Pech,et al.  The use of ethylene-suppressed lines to assess differential sensitivity to ethylene of the various ripening pathways in Cantaloupe melons , 2001 .

[9]  G. Barry,et al.  Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. , 1991, The Plant cell.

[10]  F. Lajolo,et al.  Effects of gibberellic acid on sucrose accumulation and sucrose biosynthesizing enzymes activity during banana ripening , 2003, Plant Growth Regulation.

[11]  C. S. Whitehead,et al.  The effect of ethylene and short-chain saturated fatty acids on ethylene sensitivity and binding in ripening bananas , 1991 .

[12]  R. Wills,et al.  Evidence for the function of the free radical gas — nitric oxide (NO•) — as an endogenous maturation and senescence regulating factor in higher plants , 1998 .

[13]  A. Reddy,et al.  Calcium and fruit softening: physiology and biochemistry , 1988 .

[14]  R. Tharanathan,et al.  Fruit Ripening Phenomena–An Overview , 2007, Critical reviews in food science and nutrition.

[15]  Daniel J. Cantliffe,et al.  Fruit ripening characteristics in a transgenic ‘Galia’ male parental muskmelon (Cucumis melo L. var. reticulatus Ser.) line , 2007 .

[16]  Adel A. Kader,et al.  Quality changes in fresh-cut pear slices as affected by controlled atmospheres and chemical preservatives , 2002 .

[17]  J. Pech,et al.  Down-regulation of DR12, an auxin-response-factor homolog, in the tomato results in a pleiotropic phenotype including dark green and blotchy ripening fruit. , 2002, The Plant journal : for cell and molecular biology.

[18]  J. Giovannoni,et al.  MOLECULAR BIOLOGY OF FRUIT MATURATION AND RIPENING. , 2001, Annual review of plant physiology and plant molecular biology.

[19]  E. Baldwin,et al.  EFFECT OF DIFFERENT COATING TREATMENTS ON THE QUALITY OF MANGO FRUIT , 2002 .

[20]  Influence of hydroxypropyl methylcellulose edible coating on fresh-keeping and storability of tomato , 2003 .

[21]  Ilana Urbano Bron,et al.  Influence of ripening stage on physical and chemical attributes of 'Golden' papaya fruit treated with 1-Methylcyclopropene , 2006 .

[22]  J. Pech,et al.  Recent Developments on the Role of Ethylene in the Ripening ofClimacteric Fruit , 2002 .

[23]  T. Haji,et al.  The involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening. , 2006, Journal of experimental botany.

[24]  J. R. Gorny,et al.  Controlled-atmosphere Suppression of ACC Synthase and ACC Oxidase in 'Golden Delicious' Apples during Long-term Cold Storage , 1996 .

[25]  V. Valpuesta,et al.  Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene1 , 2002, Plant Physiology.

[26]  L. Tsay,et al.  STUDIES ON THE POSTHARVEST PHYSIOLOGY OF SUGAR APPLE , 1989 .

[27]  Vidhu A. Sane,et al.  Role of Ethylene in Fruit Ripening , 2006 .

[28]  B. Glick,et al.  Bacterial ACC deaminase and the alleviation of plant stress. , 2004, Advances in applied microbiology.

[29]  Hongliang Zhu,et al.  Virus-induced gene silencing in tomato fruit. , 2005, The Plant journal : for cell and molecular biology.

[30]  D. Grierson,et al.  Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants , 1990, Nature.

[31]  Pongphen Jitareerat,et al.  Effects of cinnamon extract, chitosan coating, hot water treatment and their combinations on crown rot disease and quality of banana fruit , 2007 .

[32]  A. E. Watada,et al.  Quality of fresh-cut produce , 1999 .

[33]  B. Donèche,et al.  POSSIBLE ROLES OF BOTH ABSCISIC ACID AND INDOL-ACETIC ACID IN CONTROLLING GRAPE BERRY RIPENING PROCESS , 2007 .

[34]  M. Chinnan,et al.  Edible coating effects on storage life and quality of tomatoes , 1994 .

[35]  Raúl Herrera,et al.  Treatment with 1-MCP and the role of ethylene in aroma development of mountain papaya fruit , 2007 .

[36]  N. Hoffman,et al.  Ethylene biosynthesis and its regulation in higher plants , 1984 .

[37]  M. Serek,et al.  Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations , 1996, Plant Growth Regulation.

[38]  Zhangjun Fei,et al.  Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. , 2004, The Plant journal : for cell and molecular biology.

[39]  P. Oeller,et al.  Use of a tomato mutant constructed with reverse genetics to study fruit ripening, a complex developmental process. , 1993, Developmental genetics.

[40]  Jorge Adolfo Silva,et al.  Ácido giberélico no retardamento da maturação de caquis (Diospyrus kaki, L.), cultivar Fuyu , 2004 .

[41]  A. Ohmiya Effects of auxin on growth and ripening of mesocarp discs of peach fruit. , 2000 .

[42]  D. Grierson,et al.  Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening. , 2002, Journal of experimental botany.

[43]  A. Theologis,et al.  Early Genes and Auxin Action , 1996, Plant physiology.

[44]  A. Granell,et al.  Agroinjection of Tomato Fruits. A Tool for Rapid Functional Analysis of Transgenes Directly in Fruit1 , 2005, Plant Physiology.

[45]  S. Biondi,et al.  Peach (Prunus persica) fruit ripening: aminoethoxyvinylglycine (AVG) and exogenous polyamines affect ethylene emission and flesh firmness. , 2002, Physiologia plantarum.

[46]  E. Yahia Modified and controlled atmospheres for tropical fruits , 2006 .

[47]  S. Shiomi,et al.  Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening. , 1998, Plant physiology.

[48]  G. Sozzi,et al.  Controlled‐atmosphere storage of tomato fruit: low oxygen or elevated carbon dioxide levels alter galactosidase activity and inhibit exogenous ethylene action , 1999 .

[49]  R. Amutha,et al.  Influence of Pre and Post Harvest Chemical Treatments on Physical Characteristics of Sapota (Achras sapota L.) Var. PKM 1 , 2007 .

[50]  D. Joyce,et al.  Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags , 1999 .

[51]  J. Vrebalov,et al.  A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus , 2002, Science.

[52]  J. Totty,et al.  Effect of three lipid/hydrocolloid coatings on shelf life stability of Golden Delicious apples , 2007 .

[53]  Edna Pesis,et al.  The role of the anaerobic metabolites, acetaldehyde and ethanol, in fruit ripening, enhancement of fruit quality and fruit deterioration , 2005 .

[54]  B. Glick,et al.  Transgenic plants with altered ethylene biosynthesis or perception. , 2003, Biotechnology advances.

[55]  S. Singh,et al.  Response of climacteric-type guava (Psidium guajava L.) to postharvest treatment with 1-MCP , 2008 .

[56]  F. B. Abeles CHAPTER 6 – Fruit Ripening, Abscission, and Postharvest Disorders , 1992 .

[57]  J. Pech,et al.  Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits , 1996, Nature Biotechnology.

[58]  P. White Recent advances in fruit development and ripening: an overview. , 2002, Journal of experimental botany.

[59]  M. C. Martínez-Madrid,et al.  Polyamines, abscisic acid and ethylene production in tomato fruit , 1996 .

[60]  C. K. Ong,et al.  Isolation and expression of two pectate lyase genes during fruit ripening of banana (Musa acuminata) , 2001 .

[61]  A. Bleecker,et al.  Ethylene: a gaseous signal molecule in plants. , 2000, Annual review of cell and developmental biology.

[62]  M. Islam,et al.  Effect of Postharvest Treatments with Some Coating Materials on the Shelf Life and Quality of Banana , 2001 .

[63]  E. Buse,et al.  Ethylene-Mediated Posttranscriptional Regulation in Ripening Avocado (Persea americana) Mesocarp Discs , 1993, Plant physiology.

[64]  G. G. Sanwal,et al.  Garlic extract plus sodium metabisulphite enhances shelf life of ripe banana fruit , 2007 .

[65]  D. Grierson,et al.  Molecular biology of fruit ripening and its manipulation with antisense genes , 1992, Plant Molecular Biology.

[66]  J. Alonso,et al.  Ethylene signalling and response pathway: a unique signalling cascade with a multitude of inputs and outputs , 2005 .

[67]  M. Takata Effects of Silver Ion on the Ripening of Japanese Persimmon Fruits , 1981 .

[68]  I. Blilou,et al.  The PIN auxin efflux facilitators: evolutionary and functional perspectives. , 2005, Trends in plant science.

[69]  R. A. Kluge,et al.  Delay of ripening of ‘Pedro Sato’ guava with 1-methylcyclopropene , 2005 .

[70]  R. Herrera,et al.  Ripening of mountain papaya (Vasconcellea pubescens) and ethylene dependence of some ripening events , 2004 .

[71]  C. Bonghi,et al.  Transcriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP. , 2008, Journal of experimental botany.

[72]  J. Vrebalov,et al.  The tomato high-pigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality , 1997, Theoretical and Applied Genetics.

[73]  M. Lieberman,et al.  Influence of Plant Hormones on Ethylene Production in Apple, Tomato, and Avocado Slices during Maturation and Senescence. , 1977, Plant physiology.

[74]  D. Brummell Regulation and genetic manipulation of ripening in climacteric fruit , 2005 .

[75]  J. Pech,et al.  New members of the tomato ERF family show specific expression pattern and diverse DNA‐binding capacity to the GCC box element , 2003, FEBS letters.

[76]  Daniel Valero,et al.  Tools to Maintain Postharvest Fruit and Vegetable Quality through the Inhibition of Ethylene Action: A Review , 2007, Critical reviews in food science and nutrition.

[77]  A. Noomhorm,et al.  CONTROLLED ATMOSPHERE STORAGE of MANGO FRUIT, MANGIFERA INDICA L. cv. RAD , 1995 .

[78]  Cornelius S. Barry,et al.  Signal transduction systems regulating fruit ripening. , 2004, Trends in plant science.

[79]  C. Triggs,et al.  Relationships between maturity and storage disorders in ‘Cox's Orange Pippin’ apples as influenced by preharvest calcium or ethephon sprays , 1989 .

[80]  A. Benítez-Burraco,et al.  Cloning and characterization of two ripening-related strawberry (Fragaria x ananassa cv. Chandler) pectate lyase genes. , 2003, Journal of experimental botany.

[81]  R. McQuinn,et al.  Ethylene Insensitivity Conferred by the Green-ripe and Never-ripe 2 Ripening Mutants of Tomato1 , 2005, Plant Physiology.

[82]  Hongliang Zhu,et al.  Delay of postharvest ripening and senescence of tomato fruit through virus-induced LeACS2 gene silencing , 2006 .

[83]  Beatriz Rosana Cordenunsi,et al.  Influência do ácido giberélico na degradação do amido durante o amadurecimento da banana , 2004 .

[84]  D. Tieman,et al.  Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits , 1994, Plant physiology.

[85]  M. Gidley,et al.  Effects of exogenous oxalic acid on ripening and decay incidence in mango fruit during storage at room temperature , 2007 .

[86]  Z. Singh,et al.  Controlled atmosphere storage affects fruit ripening and quality of ‘Delta R2E2’ mango , 2005 .

[87]  W. Wagoner,et al.  Reduced ethylene synthesis by transgenic tomatoes expressing S-adenosylmethionine hydrolase , 1994, Plant Molecular Biology.

[88]  N. Pathak,et al.  Modulation of mango ripening by chemicals: physiological and biochemical aspects , 2007, Plant Growth Regulation.

[89]  Y. Ueda,et al.  Effect of modified atmosphere storage on the shelf life of banana ‘Sucrier’ , 2004 .

[90]  J. Labavitch,et al.  The linkage between cell wall metabolism and fruit softening: looking to the future , 2007 .

[91]  M. Estelle,et al.  The F-box protein TIR1 is an auxin receptor , 2005, Nature.

[92]  J. Ecker,et al.  Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. , 1998, Genes & development.

[93]  D. Joyce,et al.  1-Methylcyclopropene treatment effects on intact and fresh-cut apple , 2002 .

[94]  Vidhu A. Sane,et al.  Softening in mango (Mangifera indica cv. Dashehari) is correlated with the expression of an early ethylene responsive, ripening related expansin gene, MiExpA1 , 2005 .

[95]  J. Cohen,et al.  Measurement of Indole-3-Acetic Acid in Peach Fruits (Prunus persica L. Batsch cv Redhaven) during Development. , 1987, Plant physiology.

[96]  Shigang Liu,et al.  Effects of CO2 on respiratory metabolism in ripening banana fruit , 2004 .

[97]  Yuebiao Li,et al.  Effects of chitosan coating on postharvest life and quality of longan fruit , 2001 .

[98]  P. Tonutti,et al.  The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit , 2006 .

[99]  J. Giovannoni,et al.  The use of transgenic and naturally occurring mutants to understand and manipulate tomato fruit ripening , 1994 .

[100]  R. Wills,et al.  Use of 1-MCP to extend the time to ripen of green tomatoes and postharvest life of ripe tomatoes , 2002 .

[101]  O. Feygenberg,et al.  Ripening of Ethylene-pretreated Bananas Is Retarded Using Modified Atmosphere and Vacuum Packaging , 2005 .

[102]  G. Seymour,et al.  Pectate lyase gene expression and enzyme activity in ripening banana fruit , 2003, Plant Molecular Biology.

[103]  J. Deikman Molecular mechanisms of ethylene regulation of gene transcription , 1997 .

[104]  A. Bennett,et al.  Expression of a divergent expansin gene is fruit-specific and ripening-regulated. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[105]  Adel A. Kader,et al.  Effect of atmospheric modification, 1-MCp and chemicals on quality of fresh-cut banana , 2006 .

[106]  H. Klee,et al.  Ethylene receptor degradation controls the timing of ripening in tomato fruit. , 2007, The Plant journal : for cell and molecular biology.

[107]  María Paulina Forero STORAGE LIFE ENHANCEMENT OF AVOCADO FRUITS , 2007 .

[108]  H. Fujii,et al.  Ethylene-regulation of fruit softening and softening-related genes in peach. , 2006, Journal of experimental botany.

[109]  Ana Lúcia Soares Chaves,et al.  Ethylene and fruit ripening: from illumination gas to the control of gene expression, more than a century of discoveries , 2006 .

[110]  N. Pathak,et al.  Multiple forms of polygalacturonase from banana fruits. , 1998, Phytochemistry.

[111]  W. Gruissem,et al.  Fruits: A Developmental Perspective. , 1993, The Plant cell.

[112]  R. Fischer,et al.  Interaction of a DNA binding factor with the 5′‐flanking region of an ethylene‐responsive fruit ripening gene from tomato. , 1988, The EMBO journal.

[113]  R. Tharanathan,et al.  Polysaccharide-based composite coating formulations for shelf-life extension of fresh banana and mango , 2001 .

[114]  D. Joyce,et al.  Effect of delayed film wrapping and waxing on the shelf life of avocado fruit , 1995 .

[115]  P. Beyer,et al.  Golden Rice: introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency. , 2002, The Journal of nutrition.

[116]  S. Lurie Application of 1-methylcyclopropene to prevent spoilage , 2005 .

[117]  P. J. Davies,et al.  Inhibition of the ethylene response by 1-MCP in tomato suggests that polyamines are not involved in delaying ripening, but may moderate the rate of ripening or over-ripening. , 2006, Journal of experimental botany.

[118]  S. Blankenship,et al.  1-Methylcyclopropene Inhibits Apple Ripening , 1999 .

[119]  S. Biondi,et al.  Jasmonate-induced transcriptional changes suggest a negative interference with the ripening syndrome in peach fruit. , 2008, Journal of experimental botany.

[120]  Mondher Bouzayen,et al.  Ethylene and fruit ripening , 1997 .

[121]  Bart Nicolai,et al.  Fresh-Cut Fruits and Vegetables , 2000 .

[122]  J. Dole,et al.  1-Methylcyclopropene: a review , 2003 .

[123]  J. Walder,et al.  Gamma radiation in papaya harvested at three stages of maturation , 2004 .

[124]  A. Khan,et al.  Pre‐ or post‐harvest applications of putrescine and low temperature storage affect fruit ripening and quality of ‘Angelino’ plum , 2008 .

[125]  J. Wang,et al.  Quality and Shelf Life of Mango (Mangifera Indica L. cv. `Tainong') Coated by Using Chitosan and Polyphenols , 2007 .

[126]  S. Wyllie,et al.  Application of 1-MCP and propylene to identify ethylene-dependent ripening processes in mature banana fruit , 1998 .

[127]  A. Xiong,et al.  Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato , 2005, Plant Cell Reports.

[128]  G. Raghavan,et al.  Postharvest Storage Of Giant Cavendish Bananas Using Ethylene Oxide And Sulphur Dioxide , 2003 .

[129]  Xuetong Fan,et al.  Inhibition of ethylene action by 1-methylcyclopropene prolongs storage life of apricots. , 2000 .

[130]  V. Modi,et al.  Some Structural Changes During Ripening of Mangoes (Mangifera indica var. Alphonso) by Abscisic Acid Treatment , 1990 .

[131]  I. Kang,et al.  Foliar application of aminoethoxyvinylglycine (AVG) delays fruit ripening and reduces pre-harvest fruit drop and ethylene production of bagged “Kogetsu” apples , 2006, Plant growth regulation (Print).

[132]  Qihong Chen Optimal Control of Semilinear Elliptic Variational Bilateral Problem , 2000 .

[133]  R. Alba,et al.  Fruit-localized phytochromes regulate lycopene accumulation independently of ethylene production in tomato. , 2000, Plant physiology.

[134]  Y. Kubo,et al.  Influence of Time and Concentration of 1-MCP Application on the Shelf Life of Pear 'La France' Fruit , 2003 .

[135]  A. Hamilton,et al.  Altered fruit ripening and leaf senescence in tomatoes expressing an antisense ethylene‐forming enzyme transgene , 1993 .

[136]  R. Wills,et al.  Effect of fruit maturity on efficiency of 1-methylcyclopropene to delay the ripening of bananas , 2000 .

[137]  M. Vendrell,et al.  Development of Ethylene-synthesizing Capacity in Preclimacteric Apples: Interaction between Abscisic Acid and Ethylene , 2000 .

[138]  Da-Peng Zhang,et al.  Abscisic acid-specific binding sites in the flesh of developing apple fruit. , 2001, Journal of experimental botany.

[139]  G. G. Sanwal,et al.  Effect of phytohormones on pectate lyase activity in ripening Musa acuminata. , 2004, Plant physiology and biochemistry : PPB.

[140]  Adel A. Kader,et al.  Postharvest CO2 and ethylene production and quality maintenance of fresh-cut kiwifruit slices , 1999 .

[141]  Yuanhong Xie,et al.  Tomato Fruit Development and Ripening Are Altered by the Silencing of LeEIN2 Gene , 2006 .

[142]  M. Vendrell,et al.  Effects of nitrous oxide (N2O) treatment on the postharvest ripening of banana fruit , 2005 .

[143]  Jorge Adolfo Silva,et al.  Characterization of ripening behavior in transgenic melons expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene from apple , 2004 .

[144]  N. Tzortzakis,et al.  MAINTAINING POSTHARVEST QUALITY OF THE TOMATO FRUIT BY EMPLOYING METHYL JASMONATE AND ETHANOL VAPOR TREATMENT , 2007 .

[145]  G. Paliyath,et al.  Inhibitory effect of 1-MCP on ripening and superficial scald development in ‘McIntosh’ and ‘Delicious’ apples , 2000 .

[146]  J. Abbott,et al.  Down-Regulation of Tomato β-Galactosidase 4 Results in Decreased Fruit Softening1 , 2002, Plant Physiology.

[147]  E. Baldwin,et al.  Response of Four Apple Cultivars to 1-Methylcyclopropene Treatment and Controlled Atmosphere Storage , 2005 .

[148]  J. Giovannoni Genetic Regulation of Fruit Development and Ripening , 2004, The Plant Cell Online.

[149]  W. Schuch,et al.  Reduced ethylene synthesis in EFE antisense tomatoes has differential effects on fruit ripening processes , 1993 .

[150]  S. Sargent,et al.  Influence of 1-methylcyclopropene (1-MCP) on ripening and cell-wall matrix polysaccharides of avocado (Persea americana) fruit , 2002 .

[151]  J. Ray,et al.  Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes , 1988, Nature.

[152]  B. Akbudak,et al.  SEQUENTIAL TREATMENTS OF HOT WATER AND MODIFIED ATMOSPHERE PACKAGING IN CHERRY TOMATOES , 2007 .

[153]  P. Holford,et al.  Responses of climacteric and suppressed-climacteric plums to treatment with propylene and 1-methylcyclopropene , 1998 .

[154]  D. Valero,et al.  Exogenous Polyamines and Gibberellic Acid Effects on Peach (Prunus persica L.) Storability Improvement , 2000 .

[155]  C. Watkins The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. , 2006, Biotechnology advances.

[156]  D. Joyce,et al.  Effect of Abscisic Acid on Banana Fruit Ripening in Relation to the Role of Ethylene , 2000, Journal of Plant Growth Regulation.

[157]  S. Dinesh-Kumar,et al.  Virus-induced gene silencing in tomato. , 2002, The Plant journal : for cell and molecular biology.

[158]  A. Bennett,et al.  Modification of Expansin Protein Abundance in Tomato Fruit Alters Softening and Cell Wall Polymer Metabolism during Ripening , 1999, Plant Cell.

[159]  K K Surendranathan,et al.  Post-harvest biotechnology of fruits with special reference to banana— Perspective and scope , 2005 .

[160]  P. Civello,et al.  Influence of self-produced CO2 on postharvest life of heat-treated strawberries , 2003 .

[161]  Cornelius S. Barry,et al.  Ripening in the tomato Green-ripe mutant is inhibited by ectopic expression of a protein that disrupts ethylene signaling , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[162]  G. Casadoro,et al.  The involvement of auxin in the ripening of climacteric fruits comes of age: the hormone plays a role of its own and has an intense interplay with ethylene in ripening peaches. , 2007, Journal of experimental botany.

[163]  M. Hertog,et al.  Postharvest softening of apple (Malus domestica) fruit: A review , 2002 .

[164]  S. Blankenship,et al.  Response of apples to diazocyclopentadiene inhibition of ethylene binding , 1993 .

[165]  S. Blankenship,et al.  2, 5-Norbornadiene Retards Apple Softening , 1989, HortScience.

[166]  F. Lajolo,et al.  EFFECT OF PROTEIN SYNTHESIS INHIBITORS ON THE CLIMACTERIC OF BANANA (Musa acuminata) , 1988 .

[167]  D. Huber,et al.  Delay of avocado (Persea americana) fruit ripening by 1-methylcyclopropene and wax treatments , 2003 .

[168]  J. Pech,et al.  Ripening-associated biochemical traits of Cantaloupe Charentais melons expressing an antisense ACC oxidase transgene , 1997 .

[169]  Paxton Payton,et al.  Use of genomics tools to isolate key ripening genes and analyse fruit maturation in tomato. , 2002, Journal of experimental botany.

[170]  F. Mencarelli,et al.  Inhibition of Ethylene Synthesis and Action in Ripening Tomato Fruit by Ethanol Vapors , 1988, Journal of the American Society for Horticultural Science.