Nicotine infused into the nucleus accumbens increases synaptic dopamine as measured by in vivo microdialysis

It has been postulated that addiction to nicotine is mediated by dopamine release in the mesolimbic system. It is possible that nicotine might act directly on the dopamine terminals to release dopamine. This hypothesis was tested by infusing nicotine through a microdialysis probe into the nucleus accumbens of freely moving rats. Dopamine, dihydroxyphenylacetic acid, and homovanillic acid from the extracellular space were collected by microdialysis and measured by high pressure liquid chromatography. Nicotine increased extracellular dopamine in a dose-related manner. Systemic injection of the nicotine antagonist mecamylamine blocked the dopamine increase induced by local nicotine. These results suggest that nicotine releases dopamine by a local action in the nucleus accumbens terminal area of the mesolimbic system. Presynaptic induction of dopamine release might play a role in nicotine addiction.

[1]  G. Di Chiara,et al.  Nicotine preferentially stimulates dopamine release in the limbic system of freely moving rats. , 1986, European journal of pharmacology.

[2]  B. Hoebel,et al.  Simultaneous microdialysis and amphetamine infusion in the nucleus accumbens and striatum of freely moving rats: Increase in extracellular dopamine and serotonin , 1987, Brain Research Bulletin.

[3]  B. Hoebel,et al.  Feeding and hypothalamic stimulation increase dopamine turnover in the accumbens , 1988, Physiology & Behavior.

[4]  B. Hoebel,et al.  Phencyclidine (PCP) injected in the nucleus accumbens increases extracellular dopamine and serotonin as measured by microdialysis. , 1988, Life sciences.

[5]  G. Di Chiara,et al.  Ethanol preferentially stimulates dopamine release in the nucleus accumbens of freely moving rats. , 1985, European journal of pharmacology.

[6]  B. Hoebel,et al.  Food reward and cocaine increase extracellular dopamine in the nucleus accumbens as measured by microdialysis. , 1988, Life sciences.

[7]  A. Goldstein,et al.  Stereospecific nicotine receptors on rat brain membranes. , 1980, Science.

[8]  F. S. Radhakishun,et al.  Scheduled eating increases dopamine release in the nucleus accumbens of food-deprived rats as assessed with on-line brain dialysis , 1988, Neuroscience Letters.

[9]  T. Westfall.  Effect of nicotine and other drugs on the release of 3H-norepinephrine and 3H-dopamine from rat brain slices. , 1974, Neuropharmacology.

[10]  P. W. Bowman,et al.  PHS Public Health Service , 1963 .

[11]  G. Di Chiara,et al.  Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[12]  L. Arqueros,et al.  Nicotine-induced release of catecholamines from rat hippocampus and striatum. , 1978, Biochemical pharmacology.

[13]  B. Hoebel,et al.  A small, removable microdialysis probe. , 1986, Life sciences.

[14]  H. Imura,et al.  Nicotinic cholinergic receptors in brain synaptosomes , 1979, Brain Research.