A simple and sensitive electrochemiluminescence aptasensor for determination of ochratoxin A based on a nicking endonuclease-powered DNA walking machine.

Ochratoxin A (OTA) poses a serious threat to the health of human beings and animals. In this paper, a simple and sensitive electrochemiluminescence (ECL) aptasensor was constructed to detect OTA based on electrochemiluminescence resonance energy transfer (ECL-RET) and a nicking endonuclease-powered DNA walking machine. Originally, the signal of cadmium sulfide semiconductor quantum dots (CdS QDs) was quenched efficiently by Cy5. After the addition of OTA, the walker autonomously hybridized with Cy5-labeled DNA and released plenty of Cy5-DNA from the electrode surface with the help of a nicking endonuclease. As a result, the signal of CdS QDs recovered efficiently. As an artificial and popular signal amplification technique, the DNA walking machine greatly improved the sensitivity. Under optimal conditions, the aptasensor not only detected OTA in a linear range from 0.05 nM to 5 nM with a detection limit of 0.012 nM (S/N = 3), but also showed an excellent selectivity for OTA over other mycotoxins.

[1]  Chao Li,et al.  Simple electrochemical sensing of attomolar proteins using fabricated complexes with enhanced surface binding avidity† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc00891c Click here for additional data file. , 2015, Chemical science.

[2]  Ruo Yuan,et al.  Ultrasensitive Electrochemiluminescence Biosensor for MicroRNA Detection by 3D DNA Walking Machine Based Target Conversion and Distance-Controllable Signal Quenching and Enhancing. , 2017, Analytical chemistry.

[3]  Xingyi Huang,et al.  Colorimetric aptasensing of ochratoxin A using Au@Fe3O4 nanoparticles as signal indicator and magnetic separator. , 2016, Biosensors & bioelectronics.

[4]  Y. Ying,et al.  Recent Progress in Nanomaterial-Based Optical Aptamer Assay for the Detection of Food Chemical Contaminants. , 2017, ACS applied materials & interfaces.

[5]  Longhua Guo,et al.  An electrochemiluminescence biosensor for Kras mutations based on locked nucleic acid functionalized DNA walkers and hyperbranched rolling circle amplification. , 2017, Chemical communications.

[6]  J. Byun,et al.  An optical fiber-based LSPR aptasensor for simple and rapid in-situ detection of ochratoxin A. , 2018, Biosensors & bioelectronics.

[7]  Y. Chai,et al.  In Situ Electrochemical Generation of Electrochemiluminescent Silver Naonoclusters on Target-Cycling Synchronized Rolling Circle Amplification Platform for MicroRNA Detection. , 2016, Analytical chemistry.

[8]  Wei Li,et al.  High-Throughput Low-Background G-Quadruplex Aptamer Chemiluminescence Assay for Ochratoxin A Using a Single Photonic Crystal Microsphere. , 2017, Analytical chemistry.

[9]  D. Tang,et al.  Homogeneous electrochemical detection of ochratoxin A in foodstuff using aptamer-graphene oxide nanosheets and DNase I-based target recycling reaction. , 2017, Biosensors & bioelectronics.

[10]  J. Cruz-Aguado,et al.  Determination of ochratoxin a with a DNA aptamer. , 2008, Journal of agricultural and food chemistry.

[11]  Bin Zhou,et al.  Dual Electrochemiluminescence Signal System for In Situ and Simultaneous Evaluation of Multiple Cell-Surface Receptors. , 2017, ACS applied materials & interfaces.

[12]  Huangxian Ju,et al.  Binding-induced DNA walker for signal amplification in highly selective electrochemical detection of protein. , 2017, Biosensors & bioelectronics.

[13]  A novel universal colorimetric sensor for simultaneous dual target detection through DNA-directed self-assembly of graphene oxide and magnetic separation. , 2017, Chemical communications.

[14]  W. Kong,et al.  A self-assembly aptasensor based on thick-shell quantum dots for sensing of ochratoxin A. , 2016, Nanoscale.

[15]  Deming Kong,et al.  Ultrasensitive, label-free detection of T4 ligase and T4 polynucleotide kinase based on target-triggered hyper-branched rolling circle amplification , 2018 .

[16]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[17]  W. Guo,et al.  A novel sandwich electrochemiluminescence immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 based on immobilizing luminol on Ag@BSA core/shell microspheres. , 2016, Biosensors & bioelectronics.

[18]  Ning Gan,et al.  Microchip electrophoresis array-based aptasensor for multiplex antibiotic detection using functionalized magnetic beads and polymerase chain reaction amplification , 2018, Sensors and Actuators B: Chemical.

[19]  S. M. Taghdisi,et al.  Ultrasensitive detection of ochratoxin A using aptasensors. , 2017, Biosensors & bioelectronics.

[20]  Pu Zhang,et al.  Bi-directional DNA Walking Machine and Its Application in an Enzyme-Free Electrochemiluminescence Biosensor for Sensitive Detection of MicroRNAs. , 2017, Analytical chemistry.

[21]  E. González-Peñas,et al.  OTA-producing fungi in foodstuffs: A review , 2012 .

[22]  Weihong Tan,et al.  Direct Visualization of Walking Motions of Photocontrolled Nanomachine on the DNA Nanostructure. , 2015, Nano letters.

[23]  Weihong Tan,et al.  An autonomous and controllable light-driven DNA walking device. , 2012, Angewandte Chemie.

[24]  Carmen C. Mayorga-Martinez,et al.  Label-free impedimetric aptasensor for ochratoxin-A detection using iridium oxide nanoparticles. , 2015, Analytical chemistry.

[25]  Xiaoying Wang,et al.  On-site determination of bisphenol A in river water by a novel solid-state electrochemiluminescence quenching sensor , 2017 .

[26]  Shaojun Guo,et al.  Tuning the Aggregation/Disaggregation Behavior of Graphene Quantum Dots by Structure-Switching Aptamer for High-Sensitivity Fluorescent Ochratoxin A Sensor. , 2017, Analytical chemistry.

[27]  J. Marty,et al.  Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry , 2018, Sensors and Actuators B: Chemical.

[28]  Rongrong Wu,et al.  A sensitive solid-state electrochemiluminescence sensor for clenbuterol relying on a PtNPs/RuSiNPs/Nafion composite modified glassy carbon electrode , 2016 .

[29]  Wei Zhao,et al.  DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor. , 2017, Biosensors & bioelectronics.

[30]  Jing‐Juan Xu,et al.  Electrochemiluminescence ratiometry: a new approach to DNA biosensing. , 2013, Analytical chemistry.

[31]  G. Xie,et al.  Universal ratiometric electrochemical biosensing platform based on mesoporous platinum nanocomposite and nicking endonuclease assisted DNA walking strategy. , 2017, Biosensors & bioelectronics.

[32]  Fushen Lu,et al.  An electrochemiluminescence biosensor for endonuclease EcoRI detection. , 2017, Biosensors & bioelectronics.

[33]  Junbo Chen,et al.  Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing. , 2016, ACS nano.

[34]  Hongyuan Chen,et al.  Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection. , 2014, Analytical chemistry.

[35]  Zhijun Guo,et al.  Ochratoxin A detection platform based on signal amplification by Exonuclease III and fluorescence quenching by gold nanoparticles , 2018 .

[36]  H. Ju,et al.  Design and biosensing of Mg²⁺-dependent DNAzyme-triggered ratiometric electrochemiluminescence. , 2014, Analytical chemistry.

[37]  A. Atoui,et al.  Ochratoxin A: General Overview and Actual Molecular Status , 2010, Toxins.

[38]  Changlong Hao,et al.  Chiral Shell Core-Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin. , 2018, Small.