In vitro propagation of Hemidesmus indicus

Micropropagation of H. indicus was achieved in Murashige and Skoog's basal medium (MS) supplemented with benzyl adenine (3.0 mg/l). Addition of low concentrations of ammonium nitrate increased internodal length and thickness of shoots. Rooting of induced shoots was achieved on MS with napthalene acetic acid (1.0 0 mg/l) and kinetin (1. 0 mg/l). A simple protocol for the rapid multiplication of this important medicinal plant is described.