Distribution of neuropeptide FF‐like immunoreactivity in the brain of the lizard Gekko gecko and its relation to catecholaminergic structures

The present study provides a detailed description of the distribution of neuropeptide FF (NPFF)‐like immunoreactivity in the brain of the lizard Gekko gecko. NPFF is found to be involved in nociception, cardiovascular regulation, and endocrine function. Because of its known relationship with catecholamines in mammals, double staining with tyrosine hydroxylase (TH) antibodies was used to corroborate this for reptiles. The present study revealed that NPFF‐like‐immunoreactive (NPFF‐ir) cells and fibers were widely distributed throughout the brain. Major NPFF‐ir cell groups were observed in the diagonal band nucleus of Broca, hypothalamus, and dorsal horn of the spinal cord. Additional cells were found in the anterior olfactory nucleus, lateral and dorsal cortices, dorsolateral septum, and diencephalic intergeniculate leaflet formation. Dense plexuses of NPFF‐ir fibers were identified in the diagonal band nucleus of Broca, septum, preoptic and hypothalamic areas, isthmic region, ventrolateral tegmentum, solitary tract nucleus, and dorsolateral funiculus of the spinal cord. Extensive fiber staining also occurred in the nucleus accumbens and the midbrain tectum. Although an intimate relationship between NPFF‐ir and TH‐ir structures was obvious at many places in the brain, colocalization of these two substances was not observed. In conclusion, the distribution of NPFF in the brain of Gekko shares more features with anamniotes in terms of number of cell groups, more elaborate networks of fibers, and lack of colocalization with catecholamines than with mammals, suggesting a decrease in the distribution of this peptide in the latter vertebrate group. J. Comp. Neurol. 498:31–45, 2006. © 2006 Wiley‐Liss, Inc.

[1]  M. Pombal,et al.  Distribution of neuropeptide FF-like immunoreactive structures in the lamprey central nervous system and its relation to catecholaminergic neuronal structures , 2006, Peptides.

[2]  C. Mollereau,et al.  Opioid-modulating peptides: mechanisms of action. , 2005, Current topics in medicinal chemistry.

[3]  R. Ward,et al.  Presumptive FMRF-amide-like immunoreactive retinopetal fibres in Crocodylus niloticus , 2004, Brain Research.

[4]  F. Vandesande,et al.  Identification of 26RFa, a hypothalamic neuropeptide of the RFamide peptide family with orexigenic activity , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[5]  S. Hinuma,et al.  A New Peptidic Ligand and Its Receptor Regulating Adrenal Function in Rats* , 2003, Journal of Biological Chemistry.

[6]  H. Minakata,et al.  Novel neuropeptides related to frog growth hormone-releasing peptide: isolation, sequence, and functional analysis. , 2003, Endocrinology.

[7]  J. Nystedt,et al.  Expression of neuropeptide FF, prolactin‐releasing peptide, and the receptor UHR1/GPR10 genes during embryogenesis in the rat , 2003, Developmental dynamics : an official publication of the American Association of Anatomists.

[8]  N. Moreno,et al.  Comparative analysis of neuropeptide FF-like immunoreactivity in the brain of anuran (Rana perezi, Xenopus laevis) and urodele (Pleurodeles waltl) amphibians , 2003, Journal of Chemical Neuroanatomy.

[9]  H. Vaudry,et al.  Isolation, characterization, and distribution of a novel neuropeptide, Rana RFamide (R‐RFa), in the brain of the European green frog Rana esculenta , 2002, The Journal of comparative neurology.

[10]  K. Ukena,et al.  A novel amphibian hypothalamic neuropeptide: isolation, localization, and biological activity. , 2002, Endocrinology.

[11]  W. Smeets,et al.  Immunohistochemical localization of DARPP‐32 in the brain of the lizard, Gekko gecko: Co‐occurrence with tyrosine hydroxylase , 2001, The Journal of comparative neurology.

[12]  J. Jhamandas,et al.  New central projections of neuropeptide FF: colateral branching pathways in the brainstem and hypothalamus in the rat , 2001, Journal of Chemical Neuroanatomy.

[13]  H. Minakata,et al.  Characterization of a cDNA encoding a novel avian hypothalamic neuropeptide exerting an inhibitory effect on gonadotropin release. , 2001, The Biochemical journal.

[14]  C. Pinelli,et al.  Localization of FMRFamide-Like Immunoreactivity in the Brain of the Viviparous Skink (Chalcides chalcides) , 2001, Brain, Behavior and Evolution.

[15]  L. Puelles,et al.  Expression of calcium–binding proteins in the diencephalon of the lizard Psammodromus algirus , 2000, The Journal of comparative neurology.

[16]  W. Smeets,et al.  Catecholamine systems in the brain of vertebrates: new perspectives through a comparative approach , 2000, Brain Research Reviews.

[17]  M. Kikuchi,et al.  A novel avian hypothalamic peptide inhibiting gonadotropin release. , 2000, Biochemical and biophysical research communications.

[18]  D. L. Meyer,et al.  Comparative analysis of FMRFamide-like immunoreactivity in caiman (Caiman crocodilus) and turtle (Trachemys scripta elegans) brains , 1999, Cell and Tissue Research.

[19]  S. Hinuma,et al.  Distribution and characterization of immunoreactive prolactin-releasing peptide (PrRP) in rat tissue and plasma. , 1999, Biochemical and biophysical research communications.

[20]  Hirokazu Matsumoto,et al.  A prolactin-releasing peptide in the brain , 1998, Nature.

[21]  W. Smeets,et al.  Distribution of NADPH‐diaphorase and nitric oxide synthase in relation to catecholaminergic neuronal structures in the brain of the lizard Gekko gecko , 1997, The Journal of comparative neurology.

[22]  P. Panula,et al.  EVIDENCE FOR PROLACTIN RELEASING ACTIVITY OF NEUROPEPTIDE FF IN RATS , 1997 .

[23]  A. Aarnisalo,et al.  Neuropeptide FF, a mammalian neuropeptide with multiple functions , 1996, Progress in Neurobiology.

[24]  M. Feuilloley,et al.  Distribution of FMRFamide-like immunoreactivity in the brain of the lizard Podarcis sicula , 1994, Peptides.

[25]  P. Panula,et al.  Distribution and Characterization of Neuropeptide FF‐like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody , 1993, The European journal of neuroscience.

[26]  M. Kavaliers,et al.  Neuropeptide FF (FLQPQRFamide) and IgG from neuropeptide FF antiserum affect spatial learning in mice , 1993, Neuroscience Letters.

[27]  K. Jhamandas,et al.  Antinociceptive effects of intrathecally administered F8Famide and FMRFamide in the rat. , 1993, European journal of pharmacology.

[28]  A. Aarnisalo,et al.  Neuropeptide FF is colocalized with catecholamine-synthesizing enzymes in neurons of the nucleus of the solitary tract , 1992, Neuroscience Letters.

[29]  Hsiu‐Ying T. Yang,et al.  Mammalian FMRF-NH2-like peptide in rat pituitary: Decrease by osmotic stimulus , 1991, Peptides.

[30]  P. Panula,et al.  Origin and distribution of neuropeptide‐FF‐like immunoreactivity in the spinal cord of rats , 1991, The Journal of comparative neurology.

[31]  P. Panula,et al.  Ontogeny of the F8Famide‐like (morphine‐modulating) peptides in the central nervous system of rats , 1991, The Journal of comparative neurology.

[32]  J. L. Sims,et al.  FMRF-NH2-like mammalian octapeptide: Possible role in opiate dependence and abstinence , 1990, Peptides.

[33]  W. Smeets,et al.  New insights into the reptilian catecholaminergic systems as revealed by antibodies against the neurotransmitters and their synthetic enzymes. , 1990, Journal of chemical neuroanatomy.

[34]  W. Smeets,et al.  Distribution of phenylethanolamine-N-methyltransferase-immunoreactive perikarya and fibers in the brain of the lizard Gekko gecko. , 1990, Brain, Behavior and Evolution.

[35]  P. Panula,et al.  Rat brain regional distribution and spinal cord neuronal pathway of FLFQPQRF-NH2, a mammalian FMRF-NH2-like peptide , 1989, Brain Research.

[36]  W. Smeets,et al.  Distribution of noradrenaline immunoreactivity in the forebrain and midbrain of the lizard Gekko gecko , 1989, The Journal of comparative neurology.

[37]  R. Raffa,et al.  A-18-Famide and F-8-Famide, endogenous mammalian equivalents of the molluscan neuropeptide FMRFamide (Phe-Met-Arg-Phe-NH2), inhibit colonic bead expulsion time in mice , 1989, Peptides.

[38]  Lingzhi Fan,et al.  The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system , 1988, Neuroscience Letters.

[39]  B. Roth,et al.  Elevation of arterial pressure in rats by two new vertebrate peptides FLF QPQRF-NH2 and AGE GLSSPFWSLAAPQRF-NH2 which are immunoreactive to FMRF-NH2 antiserum , 1987, Neuropeptides.

[40]  E. Majane,et al.  Distribution and characterization of two putative endogenous opioid antagonist peptides in bovine brain , 1987, Peptides.

[41]  D. Bückmann.  [Neuropeptides in evolution]. , 1987, Verhandlungen der Deutschen Gesellschaft fur Innere Medizin.

[42]  Dr. Hendrik Jan ten Donkelaar,et al.  The Brain Stem in a Lizard, Varanus exanthematicus , 1987, Advances in Anatomy Embryology and Cell Biology.

[43]  A. Lohman,et al.  A forebrain atlas of the lizard gekko gecko , 1986, The Journal of comparative neurology.

[44]  W. Smeets,et al.  The distribution of dopamine immunoreactivity in the forebrain and midbrain of the lizard Gekko gecko: An immunohistochemical study with antibodies against dopamine , 1986, The Journal of comparative neurology.

[45]  W. Fratta,et al.  Isolation, sequencing, synthesis, and pharmacological characterization of two brain neuropeptides that modulate the action of morphine. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[46]  J. Adams.  Heavy metal intensification of DAB-based HRP reaction product. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.