Isoenzymes of Human Alkaline Phosphatase

Publisher Summary Isoenzyme refers to a biochemically distinct class of catalytically active proteins with the same specificity of bond cleavage or alteration, which can occupy several zones following electrophoresis. The chapter focuses on the mechanism of catalysis and uncompetitive inhibition and provides analyses of studies reporting distinctive immunochemical properties as well as physical properties of enzymes prepared from several organs. In the case of alkaline phosphatase, the studies rely on a combination of organ source, biochemical properties, and electrophoretic distinctions as guidelines for working in this field. Placental alkaline phosphatase is an isoenzyme of alkaline phosphatase, which can be distinguished from other alkaline phosphatases by biochemical means. The different molecular forms of this isoenzyme, which can be produced and separated by physical means, are referred as variants of placental alkaline phosphatase. Hyperphosphatasemia in diseases of liver and bone has made the serum alkaline phosphatase determination the most frequently demanded enzyme assay.

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