Characterization of post mortem arterial tissue using time-resolved photoacoustic spectroscopy at 436, 461 and 532 nm

Time-resolved photoacoustic spectroscopy has been used to characterize post mortem arterial tissue for the purpose of discriminating between normal and atheromatous areas of tissue. Ultrasonic thermoelastic waves were generated in post mortem human aorta by the absorption of nanosecond laser pulses at 436, 461 and 532 nm produced by a frequency doubled Q-switched Nd:YAG laser in conjunction with a gas filled Raman cell. A PVDF membrane hydrophone was used to detect the thermoelastic waves. At 436 nm, differences in the photoacoustic signatures of normal tissue and atherorma were found to be highly variable. At 461 nm, there was a clear and reproducible difference between the photacoustic response of atheroma and normal tissue as a result of increased optical attenuation in atheroma. At 532 nm, the generation of subsurface thermoelastic waves provided a means of determining the structure and thickness of the tissue sample. It is suggested that pulsed photoacoustic spectroscopy at 461 and 532 nm may find application in characterizing arterial tissue in situ by providing information about both the composition and thickness of the vessel wall.

[1]  T. Deutsch,et al.  Pulsed photothermal radiometry of human artery , 1987 .

[2]  M. Sigrist,et al.  Diffraction characteristics of laser‐induced acoustic waves in liquids , 1984 .

[3]  P C Beard,et al.  Extrinsic optical-fiber ultrasound sensor using a thin polymer film as a low-finesse Fabry-Perot interferometer. , 1996, Applied optics.

[4]  James F. Greenleaf,et al.  Ultrasonic Data Acquisition and Processing System for Atherosclerotic Tissue Characterization , 1974 .

[5]  M. V. van Gemert,et al.  Light dosimetry in optical phantoms and in tissues: I. Multiple flux and transport theory. , 1988, Physics in medicine and biology.

[6]  M. V. van Gemert,et al.  Optical properties of human blood vessel wall and plaque , 1985, Lasers in surgery and medicine.

[7]  S L Jacques,et al.  Fluorescence spectroscopy of turbid media: Autofluorescence of the human aorta. , 1989, Applied optics.

[8]  Richard J. Dewhurst,et al.  Photo-acoustic probe for intra-arterial imaging and therapy , 1993 .

[9]  C T Lancée,et al.  Arterial wall characteristics determined by intravascular ultrasound imaging: an in vitro study. , 1989, Journal of the American College of Cardiology.

[10]  S L Jacques,et al.  XeCl laser-induced fluorescence of atherosclerotic arteries. Spectral similarities between lipid-rich lesions and peroxidized lipoproteins. , 1993, Circulation research.

[11]  F. Dunn,et al.  Comprehensive compilation of empirical ultrasonic properties of mammalian tissues. , 1978, The Journal of the Acoustical Society of America.

[12]  D H Blankenhorn,et al.  Velocity and attenuation of sound in arterial tissues. , 1982, The Journal of the Acoustical Society of America.

[13]  M. Sigrist.  Laser generation of acoustic waves in liquids and gases , 1986 .

[14]  K. Svanberg,et al.  Malignant tumor and atherosclerotic plaque diagnosis using laser-induced fluorescence , 1990 .

[15]  Paul C. Beard,et al.  Evaluation of an optical fiber probe for in vivo measurement of the photoacoustic response of tissues , 1995, Photonics West.

[16]  R. Rava,et al.  In situ optical histochemistry of human artery using near infrared Fourier transform Raman spectroscopy. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[17]  W. Lees,et al.  The appearance of normal and abnormal arterial morphology on intravascular ultrasound. , 1993, Clinical radiology.

[18]  J. Isner,et al.  Laser Raman spectroscopy of atherosclerotic lesions in human coronary artery segments. , 1988, Applied optics.

[19]  Martin Frenz,et al.  Laser-induced microbubble formation at a fiber tip in absorbing media: experiments and theory , 1996, European Conference on Biomedical Optics.

[20]  L. Deckelbaum,et al.  Discrimination of normal and atherosclerotic aorta by laser‐induced fluorescence , 1987, Lasers in surgery and medicine.

[21]  J. Isner,et al.  Laser Raman spectroscopy of calcified atherosclerotic lesions in cardiovascular tissue. , 1987, Applied optics.

[22]  Noel A. Clark,et al.  GENERATION OF ACOUSTIC SIGNALS IN LIQUIDS BY RUBY LASER‐INDUCED THERMAL STRESS TRANSIENTS , 1964 .

[23]  L. Gournay.  Conversion of Electromagnetic to Acoustic Energy by Surface Heating , 1966 .

[24]  P. Dyer,et al.  Time‐resolved photoacoustic studies of vascular tissue ablation at three laser wavelengths , 1987 .

[25]  Lihong V. Wang,et al.  Monte Carlo Modeling of Light Transport in Multi-layered Tissues in Standard C , 1992 .

[26]  S. Jacques,et al.  Light distributions in artery tissue: Monte Carlo simulations for finite‐diameter laser beams , 1989, Lasers in surgery and medicine.

[27]  P. Dyer,et al.  Photoacoustic studies and selective ablation of vascular tissue using a pulsed dye laser , 1990 .

[28]  A. Oseroff,et al.  Preferential light absorption in atheromas in vitro. Implications for laser angioplasty. , 1986, The Journal of clinical investigation.

[29]  U. Thiemann,et al.  Analysis of the acoustic response of vascular tissue irradiated by an ultraviolet laser pulse , 1991 .

[30]  C. Patel,et al.  Pulsed optoacoustic spectroscopy of condensed matter , 1981 .

[31]  Beard Pc,et al.  Pulsed Laser Generation and Optical Fibre Detection of Thermoelastic Waves in Arterial Tissue , 1996 .