In vivo label-free microangiography by laser speckle imaging with intensity fluctuation modulation

Abstract. We present the theory of laser speckle imaging improved with intensity fluctuation modulation, where the dynamic speckle pattern can be isolated from its stationary counterpart. A series of in vivo experiments demonstrate the effectiveness of our method in achieving microangiography and monitoring vascular self-recovering process. All results show the convincing performance of our imaging method in both structural and functional imaging of blood flow, which may have potential applications in biological research and disease diagnosis.

[1]  Yaguang Zeng,et al.  Laser speckle imaging based on intensity fluctuation modulation. , 2013, Optics letters.

[2]  M. Štrucl,et al.  The effect of glibenclamide on cutaneous laser-Doppler flux. , 2008, Microvascular research.

[3]  Yuankai K. Tao,et al.  Single-pass volumetric bidirectional blood flow imaging spectral domain optical coherence tomography using a modified Hilbert transform. , 2008, Optics express.

[4]  Anthony J. Durkin,et al.  Quantitative determination of dynamical properties using coherent spatial frequency domain imaging. , 2011, Journal of the Optical Society of America. A, Optics, image science, and vision.

[5]  Li Zhang,et al.  Imaging cerebral blood flow through the intact rat skull with temporal laser speckle imaging. , 2006, Optics letters.

[6]  David L. Thomas,et al.  Measuring Cerebral Blood Flow Using Magnetic Resonance Imaging Techniques , 1999, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[7]  Ruikang K. Wang,et al.  Potential of optical microangiography to monitor cerebral blood perfusion and vascular plasticity following traumatic brain injury in mice in vivo. , 2009, Journal of biomedical optics.

[8]  J D Briers,et al.  Laser speckle contrast analysis (LASCA): a nonscanning, full-field technique for monitoring capillary blood flow. , 1996, Journal of biomedical optics.

[9]  Brett E Bouma,et al.  Characterization of Atherosclerotic Plaques by Laser Speckle Imaging , 2005, Circulation.

[10]  T. Hachiga,et al.  Blood flow velocity imaging of malignant melanoma by micro multipoint laser Doppler velocimetry , 2010 .

[11]  I. Winship,et al.  Laser Speckle Contrast Imaging of Collateral Blood Flow during Acute Ischemic Stroke , 2010, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[12]  High-resolution computed tomography of refractive index distribution by transillumination low-coherence interferometry. , 2010, Optics letters.

[13]  Qingming Luo,et al.  Lateral laser speckle contrast analysis combined with line beam scanning illumination to improve the sampling depth of blood flow imaging. , 2012, Optics letters.

[14]  Lihong V. Wang,et al.  Photoacoustic Doppler flow measurement in optically scattering media , 2007 .

[15]  M Simonutti,et al.  Holographic laser Doppler ophthalmoscopy. , 2010, Optics letters.

[16]  M. Luby,et al.  Magnetic resonance imaging and computed tomography in emergency assessment of patients with suspected acute stroke: a prospective comparison , 2007, The Lancet.