Three-dimensional image formation and restoration in an optical microscope with polarization aberrations

A major limiting factor in polarization microscopy is the rotation of the plane of polarization at oblique interfaces in the optical train, which leads to severe degradation of image quality. Polarization aberration theory has provided an effective framework for studying two- dimensional image formation and optical restoration in these systems. Here we extend the polarization aberration model to three dimensions. A model system is chosen in which polarization aberrations are optically corrected and image quality restored. Closed-form expressions for the aberrated and restored 3-D space-variant point spread functions are derived, and the contributions of the various kinds of polarization aberrations are described.

[1]  Eric W. Hansen,et al.  Restoring image quality in the polarizing microscope: analysis of the Allen video-enhanced contrast method , 1988 .

[2]  R D Allen,et al.  Video-enhanced contrast polarization (AVEC-POL) microscopy: a new method applied to the detection of birefringence in the motile reticulopodial network of Allogromia laticollaris. , 1981, Cell motility.

[3]  Russell A. Chipman,et al.  Polarization Analysis Of Optical Systems , 1988, Photonics West - Lasers and Applications in Science and Engineering.

[4]  R. Chipman,et al.  Diffraction image formation in optical systems with polarization aberrations. II: Amplitude response matrices for rotationally symmetric systems , 1991 .

[5]  G. B. Mathews,et al.  A Treatise on Bessel Functions and Their Applications to Physics. by Andrew Gray and G.B. Mathews , 1934, The Mathematical Gazette.

[6]  Russell A. Chipman,et al.  Polarization Aberrations In Optical Systems , 1987, Optics & Photonics.

[7]  W. Lewis Hyde,et al.  Studies on depolarization of light at microscope lens surfaces. II. The simultaneous realization of high resolution and high sensitivity with the polarizing microscope. , 1957 .

[8]  Russell A. Chipman,et al.  Diffraction image formation in optical systems with polarization aberrations. I - Formulation and example , 1990 .

[9]  Eric W. Hansen Overcoming Polarization Aberrations In Microscopy , 1988, Photonics West - Lasers and Applications in Science and Engineering.

[10]  E. H. Linfoot Principles of Optics , 1961 .

[11]  R. Chipman Polarization analysis of optical systems , 1989 .

[12]  Russell A. Chipman,et al.  POLARIZATION ABERRATION EFFECTS PROPAGATED IN OPTICAL SYSTEMS , 1991 .

[13]  H KUBOTA,et al.  [Diffraction images in the polarizing microscope]. , 1959, Journal of the Optical Society of America.

[14]  Shirley Dex,et al.  JR 旅客販売総合システム(マルス)における運用及び管理について , 1991 .

[15]  N. Streibl Three-dimensional imaging by a microscope , 1985 .

[16]  W. Lewis Hyde,et al.  STUDIES ON DEPOLARIZATION OF LIGHT AT MICROSCOPE LENS SURFACES , 1957, The Journal of Biophysical and Biochemical Cytology.