Phase-Contrast Hounsfield Units of Fixated and Non-Fixated Soft-Tissue Samples

X-ray phase-contrast imaging is a novel technology that achieves high soft-tissue contrast. Although its clinical impact is still under investigation, the technique may potentially improve clinical diagnostics. In conventional attenuation-based X-ray computed tomography, radiological diagnostics are quantified by Hounsfield units. Corresponding Hounsfield units for phase-contrast imaging have been recently introduced, enabling a setup-independent comparison and standardized interpretation of imaging results. Thus far, the experimental values of few tissue types have been reported; these values have been determined from fixated tissue samples. This study presents phase-contrast Hounsfield units for various types of non-fixated human soft tissues. A large variety of tissue specimens ranging from adipose, muscle and connective tissues to liver, kidney and pancreas tissues were imaged by a grating interferometer with a rotating-anode X-ray tube and a photon-counting detector. Furthermore, we investigated the effects of formalin fixation on the quantitative phase-contrast imaging results.

[1]  O. Bunk,et al.  Hard x-ray phase tomography with low-brilliance sources. , 2007, Physical review letters.

[2]  C. Grin,et al.  Determination of Preexcision Surgical Margins of Melanomas from Fixed‐Tissue Specimens , 1991, Plastic and reconstructive surgery.

[3]  F. J. Heslinga,et al.  THE ACTION OF FORMALDEHYDE SOLUTIONS ON HUMAN BRAIN LIPIDS , 1962 .

[4]  Guang-Hong Chen,et al.  Quantitative imaging of electron density and effective atomic number using phase contrast CT. , 2010, Physics in medicine and biology.

[5]  F. Pfeiffer,et al.  Phase-contrast CT: qualitative and quantitative evaluation of atherosclerotic carotid artery plaque. , 2014, Radiology.

[6]  S Auweter,et al.  Quantitative breast tissue characterization using grating-based x-ray phase-contrast imaging , 2014, Physics in medicine and biology.

[7]  R. Cloutier.  Tissue Substitutes in Radiation Dosimetry and Measurement. , 1989 .

[8]  D. R. White,et al.  The composition of body tissues. , 1986, The British journal of radiology.

[9]  P. Roller,et al.  Formaldehyde fixation. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  Marco Stampanoni,et al.  Human hand radiography using X-ray differential phase contrast combined with dark-field imaging , 2013, Skeletal Radiology.

[11]  Anders Brahme,et al.  Development of phase-contrast X-ray imaging techniques and potential medical applications. , 2008, Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics.

[12]  O. Bunk,et al.  Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources , 2006 .

[13]  C. Lawrence,et al.  Relation between size of skin excision, wound, and specimen. , 1995, Journal of the American Academy of Dermatology.

[14]  J. Carlson,et al.  Shrinkage of skin excision specimens and downcoding. , 2003, Archives of dermatology.

[15]  F. Pfeiffer,et al.  Imaging Liver Lesions Using Grating-Based Phase-Contrast Computed Tomography with Bi-Lateral Filter Post-Processing , 2014, PloS one.

[16]  Franz Pfeiffer,et al.  Quantitative phase-contrast tomography of a liquid phantom using a conventional x-ray tube source. , 2009, Optics express.

[17]  Atsushi Momose,et al.  Phase Tomography by X-ray Talbot Interferometry for Biological Imaging , 2006 .

[18]  Franz Pfeiffer,et al.  High-resolution tomographic imaging of a human cerebellum: comparison of absorption and grating-based phase contrast , 2010, Journal of The Royal Society Interface.

[19]  Franz Pfeiffer,et al.  Visualizing Typical Features of Breast Fibroadenomas Using Phase-Contrast CT: An Ex-Vivo Study , 2014, PloS one.

[20]  A. Momose.  Recent Advances in X-ray Phase Imaging , 2005 .

[21]  N. Brousse,et al.  Shrinkage of skin excision specimens: formalin fixation is not the culprit , 2009, The British journal of dermatology.

[22]  Franz Pfeiffer,et al.  Toward Clinical X-ray Phase-Contrast CT: Demonstration of Enhanced Soft-Tissue Contrast in Human Specimen , 2010, Investigative radiology.

[23]  Paola Coan,et al.  X-ray phase-contrast imaging: from pre-clinical applications towards clinics , 2013, Physics in medicine and biology.

[24]  Timm Weitkamp,et al.  Quantitative phase and absorption tomography with an X-ray grating interferometer and synchrotron radiation , 2011 .

[25]  Franz Pfeiffer,et al.  Beam hardening effects in grating-based x-ray phase-contrast imaging. , 2011, Medical physics.

[26]  R. Buesa.  Histology without formalin? , 2008, Annals of diagnostic pathology.

[27]  Franz Pfeiffer,et al.  X-ray phase imaging with a grating interferometer. , 2005, Optics express.

[28]  John A. Kiernan,et al.  Formaldehyde, Formalin, Paraformaldehyde And Glutaraldehyde: What They Are And What They Do , 2000, Microscopy Today.