Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System

A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.

[1]  R. Weinberg,et al.  The restriction point and control of cell proliferation. , 1997, Current opinion in cell biology.

[2]  H. Coller.  What's taking so long? S-phase entry from quiescence versus proliferation , 2007, Nature Reviews Molecular Cell Biology.

[3]  H Stein,et al.  Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. , 1984, Journal of immunology.

[4]  H. Niwa,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector. , 1991, Gene.

[5]  M. Reth,et al.  Regulation of B-cell proliferation and differentiation by pre-B-cell receptor signalling , 2009, Nature Reviews Immunology.

[6]  Atsushi Miyawaki,et al.  Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression , 2008, Cell.

[7]  M. Bevan,et al.  Thymocytes expressing CD8 differentiate into CD4+ cells following intrathymic injection. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Yamamura Ken-ichi,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector , 1991 .

[9]  R. Hardy,et al.  B cell development pathways. , 2001, Annual review of immunology.

[10]  R. Hardy,et al.  Identification of the earliest B lineage stage in mouse bone marrow. , 1996, Immunity.

[11]  A. Hayday,et al.  Key factors in the organized chaos of early T cell development , 2007, Nature Immunology.

[12]  A. Zetterberg,et al.  Kinetic analysis of regulatory events in G1 leading to proliferation or quiescence of Swiss 3T3 cells. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Allan R. Jones,et al.  A robust and high-throughput Cre reporting and characterization system for the whole mouse brain , 2009, Nature Neuroscience.

[14]  Timothy K Starr,et al.  Positive and negative selection of T cells. , 2003, Annual review of immunology.