Hemin-Catalyzed Peroxidation Test for Rapid Evaluation of Antioxidant Activity

A rapid method for evaluating antioxidant activity was developed. By this method as many as twenty samples can be evaluated simultaneously. Moreover, the procedure is facile and gives quick result. The test system consisted of linoleate emulsion dispersed in phosphate buffer (pH 7.0) containing hemin. The rapid oxygen uptake due to hemin-catalyzed oxidation of linoleic acid was manometrically measured with a Gilson differential respirometer. The rate of decrease in the oxygen uptake was used for evaluating antioxidant activity of samples. The activity of browning products formed by the reaction between amino acid and sugar was determined by this method, and the results were compared with those obtained by the active oxygen method.