The effect of calcium on the force‐velocity relation of briefly glycerinated frog muscle fibres

1. Twitch fibres were isolated from the semitendinosus muscles of frogs. The sarcolemma was made more permeable by a 30 min soak in a solution containing 47·3% glycerine (v/v), 2 m M‐EGTA and 10 m M phosphate buffer, pH 7. This was followed by a 30–60 min soak in a solution containing the non‐ionic detergent Lubrol‐WX. The fibres were then placed in a relaxing medium containing (in m M): KCl, 100; MgCl2, 1; ATP, 4; EGTA, 2; imidazole buffer, 10; pH 7·0.

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