论文信息 - Kinetics of zinc and cadmium dissociation of metallothionein in the presence of chelating agents

Kinetics of zinc and cadmium dissociation of metallothionein in the presence of chelating agents

The dissociation kinetics of metal ions from native metallothionein (Cd5Zn2-MT) of rat liver has been studied in the presence of external ligands including amino acids, peptide and polyamino polycarboxylic acids at 25°C. The study shows that the solution pH affects the metal dissociation process. Different ligands present different multiphasic kinetics. The possible mechanism of metal dissociation from metallothionein has been proposed.

Z. Huang | Hong-Yu Hu | Wei‐Qiang Gu

[1] David H. Petering,et al. Structure—Reactivity Relationships of Metallothionein, a Unique Metal-Binding Protein , 1989 .

[2] M. Stillman,et al. Cadmium binding to metallothioneins. Domain specificity in reactions of alpha and beta fragments, apometallothionein, and zinc metallothionein with Cd2+. , 1987, The Journal of biological chemistry.

[3] B. Wang,et al. Crystal structure of Cd,Zn metallothionein. , 1985, Science.

[4] R. Cousins. Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. , 1985, Physiological reviews.

[5] H. Hill,et al. Zinc(II), cadmium(II), and mercury(II) thiolate transitions in metallothionein. , 1981, Biochemistry.

[6] D. Petering,et al. Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[7] F. O. Brady,et al. Reactivation in vitro of zinc-requiring apo-enzymes by rat liver zinc-thionein. , 1980, The Biochemical journal.

[8] D. Winge,et al. Metal-induced formation of metallothionein in rat liver. , 1975, Archives of biochemistry and biophysics.

[9] R J Williams,et al. Metalloenzymes: the entatic nature of their active sites. , 1968, Proceedings of the National Academy of Sciences of the United States of America.