Effects of Electrical Stimulation on Myofibrillar Proteins and Tenderness of Beef Muscle

The proteolysis of Eight Holstein steers, were slaughtered. ES of low voltage (40V, was done one pairs of steers for 30, 60, and 90 sec, respectively within 5 min after slaughter. At 3 hr post-mortem, samples of Biceps femoris muscles were obtained. Each muscle was packed and stored at 1±1 o C within 3 or 4 hr after slaughter. Muscles were then analyzed at 0, 3, 5, 7, 14, and 21 days. After storage. SDS-PAGE of the myofibrillar proteins was performed Warner-Bratzler shear force values were measured