Synthesis , characterization and antimicrobial activity of steroidal ( 6 R )-spiro-∆ 2 ' , 1 ' , 3 ' , 4 ' -

A series of new steroidal (6R)-spiro-∆ 2 `, 1`, 3`, 4`-oxadiazolines (7-9) have been synthesized via the reaction of steroidal semicarbazones (4-6) with acetic anhydride and pyridine at 80 0 C. The structures of the newly synthesized compounds have been established on the basis of their spectral (IR, 1 H NMR, 13 C NMR, MS) and analytical studies. Antimicrobial activity assessment of the synthesized compounds has been evaluated against different bacterial as well as fungal strains. Difference in the potency of activity against the strains was evaluated on the basis of SAR, and it has been revealed that substitution at 3β-positions in steroidal oxadiazolines enhanced the antimicrobial activities.

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