Molecular detection of two cassava Begomoviruses in some parts of Southern Nigeria

Cassava mosaic disease (CMD), caused by an array of is the most economically important viral disease of cassava in sub-Saharan Africa. The most frequently reported in West Africa are African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV). In this study, 42 cassava leaves and 30 symptomatic weeds belonging to the Asteraceae, Cucurbitaceae and Leguminosae families were collected from backyard gardens in Edo, Ondo, Anambra, and Delta States in 2009. Deoxyribonucleic acid (DNA) extracts from these leaves were tested for ACMV and EACMCV in a multiplex polymerase chain reaction (PCR) assay. The PCR primers used were designed to amplify the replicase regions of DNA-A components of both viruses. Most of the cassava plants within the survey area were either symptomless or showed mild symptoms. ACMV was detected in 16% of cassava leaves from Edo State but not in any of the cassava leaves from the other three states. One weed sample each from Edo State (5.56%) and Ondo State (10%) were also positive for ACMV. EACMCV was not detected in any of the samples tested. The low virus occurrence observed from PCR results and the observed low incidence of the CMD characteristic mosaic symptoms on cassava leaves in the states sampled may be attributed to the use of CMD resistant or tolerant cassava varieties, and may be a result of the massive distribution of virus resistant cassava cuttings to these States by the International Institute of Tropical Agriculture (IITA).   Key words: African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), multiplex polymerase chain reaction (PCR).

[1]  C. Bragard,et al.  Dual infection by cassava begomoviruses in two leguminous species (Fabaceae) in Yangambi, Northeastern Democratic Republic of Congo , 2010, Archives of Virology.

[2]  R. Naidu,et al.  Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava. , 2008, Journal of virological methods.

[3]  R. Naidu,et al.  Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria , 2008, Archives of Virology.

[4]  J. Brown,et al.  Geminivirus strain demarcation and nomenclature , 2008, Archives of Virology.

[5]  J. Legg African cassava mosaic disease , 2008 .

[6]  James B. Hicks,et al.  A plant DNA minipreparation: Version II , 1983, Plant Molecular Biology Reporter.

[7]  R. Ortiz,et al.  Cassava improvement: challenges and impacts , 2006, The Journal of Agricultural Science.

[8]  A. Dixon,et al.  Status of Cassava Begomoviruses and Their New Natural Hosts in Nigeria. , 2006, Plant disease.

[9]  R. Cooter,et al.  Strategies for controlling cassava mosaic virus disease in Africa , 2005 .

[10]  A. Dixon,et al.  Molecular Variability and Distribution of Cassava Mosaic Begomoviruses in Nigeria , 2005 .

[11]  C. Fauquet,et al.  Cassava mosaic geminiviruses in Africa , 2004, Plant Molecular Biology.

[12]  C. Fauquet,et al.  Geminivirus classification and nomenclature: progress and problems , 2003 .

[13]  A. Dixon,et al.  Variants of East African cassava mosaic virus and Its Distribution in Double Infections with African cassava mosaic virus in Nigeria. , 2003, Plant disease.

[14]  J. Legg,et al.  Cassava virus diseases in Africa , 2003 .

[15]  A. Dixon,et al.  Cassava mosaic disease and its causal agents: the Nigeria situation , 2003 .

[16]  C. Fauquet,et al.  Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon. , 2000, The Journal of general virology.

[17]  Yule Liu,et al.  Role of a novel type of double infection in the geminivirus‐induced epidemic of severe cassava mosaic in Uganda , 1997 .

[18]  P. Pellett Food and agricultural organization of the United Nations : Technical report , 1996 .

[19]  S. O'hair Tropical root and tuber crops. , 1990 .